利用人工介质改善太湖水源地水质的研究

采用组合介质、弹性介质、无纺布介质等3种人工介质进行了改善太湖水源地水质的中试研究.结果表明,组合介质上的生物量及生物活性分别为（26.99±9.4）μgP/g和（127.13±79.4）μgTF/（g·h）,均高于无纺布介质和弹性介质的,且其净化效果更佳,是较为适用的人工介质.组合介质通过富集微生物来净化水质,对微量有机物的去除效果明显.当HRT为7d、组合介质的填充率为26.8%时,对TOC、CODMn、Chl-a、藻毒素的平均去除率分别为42.1%、22.5%、71.9%、67.9%.     

Surface treatment of NiTi shape memory alloy by modified advanced oxidation process

A modified advanced oxidation process(AOP) utilizing a UV/electrochemically-generated peroxide system was used to fabricate titania films on chemically polished NiTi shape memory alloy(SMA). The microstructure and biomedical properties of the film were characterized by scanning electron microscopy(SEM), X-ray photoelectron spectroscopy(XPS), inductively-coupled plasma mass spectrometry(ICPMS), hemolysis analysis, and blood platelet adhesion test. It is found that the modified AOP has a high processing effectiveness and can result in the formation of a dense titania film with a Ni-free zone near its top surface. In comparison, Ni can still be detected on the outer NiTi surface by the conventional AOP using the UV/H2O2 system. The depth profiles of O, Ni, Ti show that the film possesses a smooth graded interface structure next to the NiTi substrate and this structure enhances the mechanical stability of titania film. The titania film can dramatically reduce toxic Ni ion release and also improve the hemolysis resistance and thromboresistance of biomedical NiTi SMA.     

镍钛合金光催化高级氧化表面改性与生物相容性

在UV/H2O2光催化体系中,通过高级氧化法在NiTi SMA表面生成贫Ni的Ti氧化膜.通过亲水性试验、血小板黏附试验以及MTT细胞毒性试验对改性后试样的生物相容性进行了系统的评价,研究发现改性后试样的亲水性得到明显改善.表面血小板黏附数量减少,变形和团聚现象得到抑制,形成血栓的可能性降低,血液相容性得到提高.改性后试样的细胞相对增殖率(RGR)高于化学抛光的试样,试样周围细胞分裂增生良好,与材料边缘结合自然,改性处理同样提高了试样的细胞相容性.结果表明光催化高级氧化是提高NiTi SMA生物相容性的一种有效方法.     

In vitro biocompatibility of titanium-nickel alloy with titanium oxide film by H2O2 oxidation

Titanium oxide film with a graded interface to NiTi matrix was synthesized in situ on NiTi shape memory alloy（SMA） by oxidation in H2O2 solution. In vitro studies including contact angle measurement, hemolysis, MTT cytotoxicity and cell morphology tests were employed to investigate the biocompatibility of the H2O2-oxidized NiTi SMAs with this titanium oxide film. The results reveal that wettability, blood compatibility and fibroblasts compatibility of NiTi SMA are improved by the coating of titanium oxide film through H2O2 oxidation treatment.     

骨组织工程用PLGA多孔支架的制备及细胞毒性研究

制备能在骨组织工程研究中应用,并具有良好孔隙结构的块状聚（D,L-乳酸-CO-乙醇酸）（PLGA）多孔支架,探索出以冰粒子作为致孔剂,采用粒子滤出方法结合冷冻干燥工艺制备多孔支架的方法.首先将冰颗粒加入预冻的PLGA氯仿溶液中混合均匀,然后把混合物置于液氮中深度冷冻后冷冻干燥,制得多孔支架.对支架孔隙结构分析表明,该工艺制备的多孔支架无致孔剂残留、三维结构良好、孔径与孔隙可通过改变冰粒子的粒径和质量分数来控制;细胞毒性实验表明该多孔支架毒性在0～1级,可作为骨组织工程研究用多孔支架.     

生物滤池中生物量与生物活性分析及其净水效果

通过平板计数法(HPC)、脂磷法(Lipid-P)和脱氢酶法(TTC-DHA)对4种生物滤池中生物量和生物活性的空间分布进行分析,并结合出水水质考察各生物滤池的净水效果.结果表明,4种生物滤池中的生物量(HPC、Lipid-P)呈显著的成层分布,并随滤层深度的增加而降低;在同一滤层高度,球状活性炭滤料的HPC和Lipid-P生物量均高于柱状活性炭滤料的;4种生物滤池的HPC和Lipid-P生物量均与脱氢酶生物活性有较好的相关性(R2>0.706).球状活性炭生物滤池和强化球状活性炭生物滤池中的标准化生物活性(单位生物量的生物活性)随滤层深度的增加而增加,且球状活性炭生物滤池的净水效果优于柱状活性炭生物滤池的.因此,使用活性炭作为生物滤池的滤料时应合理选择炭型.