CaDHN3, a Pepper (Capsicum annuum L.) Dehydrin Gene Enhances the Tolerance against Salt and Drought Stresses by Reducing ROS Accumulation

Dehydrins (DHNs) play an important role in abiotic stress tolerance in a large number of plants, but very little is known about the function of DHNs in pepper plants. Here, we isolated a Y₁SK₂-type DHN gene “CaDHN3” from pepper. To authenticate the function of CaDHN3 in salt and drought stresses, it was overexpressed in Arabidopsis and silenced in pepper through virus-induced gene silencing (VIGS). Sub-cellular localization showed that CaDHN3 was located in the nucleus and cell membrane. It was found that CaDHN3-overexpressed (OE) in Arabidopsis plants showed salt and drought tolerance phenotypic characteristics, i.e., increased the initial rooting length and germination rate, enhanced chlorophyll content, lowered the relative electrolyte leakage (REL) and malondialdehyde (MDA) content than the wild-type (WT) plants. Moreover, a substantial increase in the activities of antioxidant enzymes; including the superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), ascorbate peroxidase (APX), and lower hydrogen peroxide (H₂O₂) contents and higher O₂^•− contents in the transgenic Arabidopsis plants. Silencing of CaDHN3 in pepper decreased the salt- and drought-stress tolerance, through a higher REL and MDA content, and there was more accumulation of reactive oxygen species (ROS) in the CaDHN3-silenced pepper plants than the control plants. Based on the yeast two-hybrid (Y2H) screening and Bimolecular Fluorescence Complementation (BiFC) results, we found that CaDHN3 interacts with CaHIRD11 protein in the plasma membrane. Correspondingly, the expressions of four osmotic-related genes were significantly up-regulated in the CaDHN3-overexpressed lines. In brief, our results manifested that CaDHN3 may play an important role in regulating the relative osmotic stress responses in plants through the ROS signaling pathway. The results of this study will provide a basis for further analyses of the function of DHN genes in pepper.     

Synthesis,characterization and magnetic properties of polythiophene/SrFe12-x(ZrZn)0.5xO19 nanocomposite as a microwave absorber

Ferrites are an important group of magnetic materials which are used as absorbers. The incorporation of ferrite and conducting polymer achieves great enhancement in microwave absorption properties. The nanocomposites of hexagonal ferrites embedded by conducting polymers such as polypyrrole, polyaniline and polythiophene (PTH) have been paid much attention. In the present study, strontium hexagonal ferrite doped by Zr and Zn with the final formula of SrFe_12-x(ZrZn)_0.5xO19 considering x = 0.9 and embedded by PTH was produced to achieve a nanocomposite with the highest microwave absorbing ability. In this study, after synthesis of SrFe₁₂O₁₉(ZrZn)_0.5xO19 and PTH, the nanocomposite was prepared by in situ polymerization. Wrapping the ferrite particles and PTH chains could form nanocomposite properly, and therefore acceptable interactions were observable between SrFe_12-x(ZrZn)_0.5xO19ferrite particles and PTH polymer chains in the composites. Assessing the X-ray diffraction (XRD) patterns of SrFe_12-x(ZrZn)_0.5xO19, PTH, and PTH/SrFe_12-x(ZrZn)_0.5xO19 nanocomposite indicated that the PTH characteristic peak shifts slightly and its peak intensity reduces, which may be attribute to the coating of PTH polymer chains onto SrFe_12-x(ZrZn)_0.5xO19 particles. We revealed also lower magnetic properties in the obtained nanocomposite. The morphological assessment also suggested that PTH could effectively coat the SrFe_12-x(ZrZn)_0.5xO19 particles. The synergistic effect of SrFe_12-x(ZrZn)_0.5xO19 particle plus PTH leads to microwave absorption percentage higher than 95% by PTH/SrFe_12-x(ZrZn)_0.5xO19 nanocomposite. Overall, nanocomposite creating by coupling interaction between SrFe_12-x(ZrZn)_0.5xO19 particles (x = 0.9) and PTH can effectively lead to achieve the highest rate of absorption of electromagnetic waves.     

Incorporating microgrids coupling with utilization of flexible switching to enhance self-healing ability of electric distribution systems

Electric distribution networks have to deal with issues caused by natural disasters. These problems possess unique characteristics, and their severity can make load restoration methods impotent. One solution that can help in alleviating the aftermath is the use of microgrids (MGs). Employing the cumulative capacity of the generation resources through MG coupling facilitates the self-healing capability and leads to better-coordinated energy management during the restoration period, while the switching capability of the system should also be considered. In this paper, to form and schedule dynamic MGs in distribution systems, a novel model based on mixed-integer linear programming (MILP) is proposed. This approach employs graph-related theories to formulate the optimal formation of the networked MGs and management of their proper participation in the load recovery process. In addition, the Benders decomposition technique is applied to alleviate computability issues of the optimization problem. The validity and applicability of the proposed model are evaluated by several simulation studies.     

Hydrogen production employing Cu(BDC) metal–organic framework support in methanol steam reforming process within monolithic micro-reactors

Cu(BDC) metal–organic framework (MOF) was used as a support for the copper (Cu) catalyst applied in the methanol steam reforming (MSR) process at low temperatures (130–250 °C) with a feed WHSV = 9.2 h^?1 within the monolithic reactor. Also, the effects of diverse promoters were examined on the catalytic activities of the Cu/X–Cu(BDC) (X = Ce, Zn, Gd, Sm, La, Y, Pr) catalysts. Results showed that the Ce/Sm–Cu(BDC) supports exhibited highest activities, lowest reduction temperatures and largest specific surface areas, which caused highest distributions of the active copper metal nanoparticles on the supports. The reactor tests displayed that the activities of Cu/X–Cu(BDC) (X = Ce, Zn, Gd, Sm, La, Y, Pr) catalysts followed the order X = Ce > Sm > Y > La > Pr > Cu(BDC) > Zn > Gd. The highest activities of Ce and Sm containing catalysts were attributed to the presence of CeO₂ and Sm₂O₃ caused the oxygen vacancies on the catalyst surface which had positive effects on the methanol reforming process. The time-on-stream stability tests showed the highest resistance of the Cu/Ce–Cu(BDC) catalyst to the carbon formation during 32 h. Consequently, the Cu/Ce–Cu(BDC) with the highest stability, methanol conversion and carbon monoxide selectivity could be used in practical industrial applications.     

Effect of hexagonal structure nanoparticles on the morphological performance of the ceramic scaffold using analytical oscillation response

Porous bony scaffolds are utilized to manage the growth and migration of cells from adjacent tissues to a defective position. In the current investigation, the effect of titanium oxide (TiO₂) nanoparticles on mechanical and physical properties of porous bony implants made of polymeric polycaprolactone (PCL) is studied. The bio-nanocomposite scaffolds are prepared with composition of nanocrystalline hydroxyapatite (HA) and TiO₂ powder using the freeze-drying technique for different weight fractions of TiO₂ (0 wt%, 5 wt%, 10 wt%, and 15 wt%). In order to identify the microstructure and morphology of the fabricated porous bio-nanocomposites, the X-ray diffraction (XRD), atomic force microscope (AFM) and scanning electron microscopy (SEM) are employed. Also, the biocompatibility and biodegradability of the manufactured scaffolds are examined by placing them in a simulated body fluid (SBF) for 21 days, their weight and pH changes are measured. The rate of degradation of the PCL-HA scaffold can be controlled by varying the percentage of its constituent components. Due to an increasing growth and activity of bone cells and the apatite formation on the free surface of the fabricated bio-nanocomposite implants as well as their reasonable mechanical properties, they have the potential to be used as a bone substitute. Additionally, with the aid of the experimentally extracted mechanical properties of the scaffolds, the vibrational characteristics of a beam-type implant made of the proposed porous bio-nanocomposites are explored. The results obtained from SEM image indicate that the scaffolds produced by the employed method have high total porosity (70%–85%) and effective porosity. The pore size is obtained between 60 and 200 μm, which is desirable for the growth and propagation of bone cells. Also, it is revealed that the addition of TiO₂ nanoparticles leads to reduce the rate of dissolution of the fabricated bio-nanocomposite scaffolds.     

Synthesis,characterization and adsorption studies of h-BN crystal for efficient removal of Cd2+ from aqueous solution

In the present study, hexagonal boron nitride (h-BN) was synthesized from boric acid and melamine by thermal annealing method in a nitrogen atmosphere. The pure h-BN was used as an efficient sorbent for the uptake of Cd²⁺ ions from the solution phase. The kinetics and sorption studies of metal ions onto the h-BN were carried out in batch adsorption experiments at different temperature, time, pH, sorbent dosage, and concentration of metal ions. The optimum pH for the removal of the Cd²⁺ ions was found to be pH 7. The effect of temperature showed that the process of Cd²⁺ sorption remained endothermic in the range of 298 K–328 K. The Lagergren's first and Ho's second kinetic models were tested to interpret the adsorption kinetic data, however the present data was explained well by Ho's model for kinetics. The thermodynamic perameters ΔG, ΔS and ΔH were determined using the available adsorption data at different temperatures. The physicochemical properties of the synthesized product were also characterized before and after adsorption by different analytical techniques like FT-IR, TGA, XRD and Point of Zero Charge (PZC). The morphology of the surface was analyzed with the help of Scanning Electron Microscopy. The h-BN proved to be an efficient adsorbent for the uptake of the Cd²⁺ ions from aqueous media.     

Bioavailability,rheology, and sensory evaluation of mayonnaise fortified with vitamin D encapsulated in protein-based carriers

Vitamin D lost its functionality during processing and storage, thus, encapsulation with proteins is desirable to preserve bioactivity. The aim of the current study was to develop encapsulated vitamin D fortified mayonnaise (VDFM) using whey protein isolates (WPI) and soy protein isolates (SPI) as encapsulating materials in three different formulations, that is, 10% WPI, 10% SPI, and 5/5% WPI/SPI. Increased shear stress decreased the apparent viscosity along with significant effects on the loss modulus of VDFM. WPI encapsulates showed better results as compared to SPI. WPI based VDFM (M₁) depicted the best results in terms of size and dispersion uniformity of oil droplets. Hue angle and total change differed significantly among treatments. The highest value for overall acceptability was acquired by M₃ (5:5%WPI:SPI-encapsulates) thus proceed for in vivo trials. Serum vitamin D level was significantly higher in the encapsulated VDFM rat group (58.14 ± 6.29 nmol/L) than the control (37.80 ± 4.98 nmol/L). Conclusively, WPI and SPI encapsulates have the potential to improve the stability and bioavailability of vitamin D.     

Benefitting from High-κ Spacer Engineering in Balistic Triple-Gate Junctionless FinFET- a Full Quantum Study

In this paper, a numerically comprehensive investigation have been performed in order to propose a high-κ spacer triple-gate junctionless FinFET (HKS     

Dynamically vulcanized thermoplastic elastomer nanocomposites based on linear low-density polyethylene/styrene-butadiene rubber/nanoclay/bitumen: morphology and rheological behavior

Dynamically crosslinked thermoplastic elastomer nanocomposites were synthesized as modifier for the bitumen binder-based asphalts. Linear low-density polyethylene (LLDPE) and styrene-butadiene rubber (SBR), with the ratio of 80/20, bitumen, and organically modified clay (OC) were all melt mixed in the presence of the sulfur curing system. The proposed mixing was carried out in an internal mixer at 160 °C with a rotor speed of 120 rpm. To enhance the molecular interactions between the polymer phases and the clay silicate layers, maleic anhydride-grafted LLDPE (PE-g-MA) with the maleiation degree of 50% was also incorporated into the mixture. Observation of the composite samples, using the scanning electron microscopy (SEM), revealed the matrix dispersed type of morphology for all dynamically vulcanized samples. X-ray diffraction (XRD) and transmission electron microscopy (TEM) examinations evidenced the exfoliation of the clay silicate layers with good dispersion. Rheomechanical spectrometry (RMS) was performed on the prepared nanocomposites. All dynamically vulcanized nanocomposites comprising 2.5% of OC exhibited shear-thinning behavior and non-terminal characteristics with a low frequency range. These indicate the formation of three-dimensional physical networks by the clay nanolayers throughout the LLDPE matrix. The presence of the bitumen in the composition of the prepared nanocomposites improved the flowability of the samples. This is a promising feature of the prepared nanocomposites to be used as an elastic and resistant modifier in the composition of the bitumen-based asphalts.

     

Application of artificial neural network for prediction of particle size in pharmaceutical cocrystallization using mechanochemical synthesis

Neural Computing and Applications - Milling by mechanical means is vital unit operation in pharmaceutical processing which can be used for controlling particle size reduction. This approach can be...