Quantitative biology of single neurons

The building blocks of complex biological systems are single cells. Fundamental insights gained from single-cell analysis promise to provide the framework for understanding normal biological systems development as well as the limits on systems/cellular ability to respond to disease. The interplay of cells to create functional systems is not well understood. Until recently, the study of single cells has concentrated primarily on morphological and physiological characterization. With the application of new highly sensitive molecular and genomic technologies, the quantitative biochemistry of single cells is now accessible.     

A CHIM-based interactive Tchebycheff procedure for multiple objective decision making

This study presents a new solution procedure for multiple objective programming (MOP). It applies the concept of the normal boundary intersection (NBI) within the framework of the interactive weighted Tchebycheff procedure (IWTP). The proposed procedure is a collaborative approach to overcome the weak points inherent in both the NBI method and IWTP. In order to control the solution procedure of pinpointing a final solution properly, we parameterized the Pareto-frontier via a set of reference point vectors based on the convex hull of individual maxima (CHIM) instead of using the varying weights of each objective. Using well-distributed reference point vectors, we could identify well-distributed Pareto-optimal solutions, thereby eliminating the IWTP filtering stages and reducing the chance of missing the best compromise solution from the decision maker (DM)'s utility point of view. Moreover, by working with a sequence of progressively smaller subsets of reference point vectors, the DM can identify a final solution at earlier stages than with the IWTP.     

Temperature Dependence of Efficiency in GaInN/GaN Light‐Emitting Diodes with a GaInN Underlayer

The temperature dependence of the efficiency (i.e., temperature‐mediated efficiency droop) in blue light‐emitting diodes (LEDs) is investigated. A GaInN/GaN LED with a GaInN underlayer having an indium mole fraction of 8% shows less temperature dependence of efficiency, compared to the LED without an underlayer. Better carrier confinement in the active region of the LED with a GaInN underlayer is proposed to reduce carrier leakage from the active region at high temperature. The results indicate that the insertion of an underlayer leads to an improvement of the LED's radiative efficiency and its high‐temperature‐tolerant performance.     

Expression and localization of two-pore domain K(+) channels in bovine germ cells

Two-pore domain K(+) (K(2P)) channels that help set the resting membrane potential of excitable and nonexcitable cells are expressed in many kinds of cells and tissues. However, the expression of K(2P) channels has not yet been reported in bovine germ cells. In this study, we demonstrate for the first time that K(2P) channels are expressed in the reproductive organs and germ cells of Korean cattle. RT-PCR data showed that members of the K(2P) channel family, specifically KCNK3, KCNK9, KCNK2, KCNK10, and KCNK4, were expressed in the ovary, testis, oocytes, embryo, and sperm. Out of these channels, KCNK2 and KCNK4 mRNAs were abundantly expressed in the mature oocytes, eight-cell stage embryos, and blastocysts compared with immature oocytes. KCNK4 and KCNK3 were significantly increased in eight-cell stage embryos. Immunocytochemical data showed that KCNK2, KCNK10, KCNK4, KCNK3, and KCNK9 channel proteins were expressed at the membrane of oocytes and blastocysts. KCNK10 and KCNK4 were strongly expressed and distributed in oocyte membranes. These channel proteins were also localized to the acrosome sperm cap. In particular, KCNK3 and KCNK4 were strongly localized to the post-acrosomal region of the sperm head and the equatorial band within the sperm head respectively. These results suggest that K(2P) channels might contribute to the background K(+) conductance of germ cells and regulate various physiological processes, such as maturation, fertilization, and development.     

Consideration of the Actual Current-Spreading Length of GaN-Based Light-Emitting Diodes for High-Efficiency Design

Based on the proposed experimental method, the current spreading length of GaN-based light-emitting diodes (LEDs) was measured and analyzed for practical device design. In this study, Thompson's and Guo's models, which are categorized according to vertical series resistance (in particular, p-type contact resistance), were used to extract device parameters. It was shown that the measured current spreading length strongly depends on the injected current density. For LEDs fabricated with low-resistance p-type contacts, this behavior could be explained in terms of the accelerated current crowding with higher current densities occurring as a result of the reduced voltage drop across the junction, which is in good agreement with Thompson's relation. However, for LEDs fabricated with high-resistance p-contacts, unlike Guo's prediction, the measured current spreading length also showed a strong dependence on the injected current density. This was attributed to thermal heating at the p-contact, resulting in the reduction of the voltage drop across the p-contact and so junction voltage, which is also in agreement with Thompson's model. Based on the measured parameters and the design rule, efficient p-type reflectors, namely, hybrid reflectors were designed. Compared with conventional ones, LEDs fabricated with the hybrid reflectors exhibited better output power at a reasonable forward voltage, indicating that the proposed method is effective in understanding the actual current spreading and hence the practical design of high-efficiency LEDs.     

Multiple change-point detection of multivariate mean vectors with the Bayesian approach

Bayesian multiple change-point models are proposed for multivariate means. The models require that the data be from a multivariate normal distribution with a truncated Poisson prior for the number of change-points and conjugate priors for the distributional parameters. We apply the stochastic approximation Monte Carlo (SAMC) algorithm to the multiple change-point detection problems. Numerical results show that SAMC makes a significant improvement over RJMCMC for complex Bayesian model selection problems in change-point estimation.     

Muscimol Directly Activates the TREK-2 Channel Expressed in GABAergic Neurons through Its N-Terminus

The two-pore domain K⁺ (K_2P) channel, which is involved in setting the resting membrane potential in neurons, is an essential target for receptor agonists. Activation of the γ-aminobutyric acid (GABA) receptors (GABA_AR and GABA_BR) reduces cellular excitability through Cl^- influx and K⁺ efflux in neurons. Relatively little is known about the link between GABA_AR and the K⁺ channel. The present study was performed to identify the effect of GABAR agonists on K_2P channel expression and activity in the neuroblastic B35 cells that maintain glutamic acid decarboxylase (GAD) activity and express GABA. TASK and TREK/TRAAK mRNA were expressed in B35 cells with a high level of TREK-2 and TRAAK. In addition, TREK/TRAAK proteins were detected in the GABAergic neurons obtained from GABA transgenic mice. Furthermore, TREK-2 mRNA and protein expression levels were markedly upregulated in B35 cells by GABA_AR and GABA_BR agonists. In particular, muscimol, a GABA_AR agonist, significantly increased TREK-2 expression and activity, but the effect was reduced in the presence of the GABA_AR antagonist bicuculine or TREK-2 inhibitor norfluoxetine. In the whole-cell and single-channel patch configurations, muscimol increased TREK-2 activity, but the muscimol effect disappeared in the N-terminal deletion mutant. These results indicate that muscimol directly induces TREK-2 activation through the N-terminus and suggest that muscimol can reduce cellular excitability by activating the TREK-2 channel and by inducing Cl^- influx in GABAergic neurons.     

Microbial assessment of medicinal herbs (Cnidii Rhizoma and Alismatis Rhizoma), effects of electron beam irradiation and detection characteristics

Food Science and Biotechnology - Medicinal herbs comprise of heavy microbial contaminations. This study aimed to assess microbial hazards including foodborne pathogens in 20 commercial medicinal...