A PIXE METHOD TO STUDY BIOLOGY AT THE DNA LEVEL

An external proton beam system with a detection sensitivity of 10 g for ruthenium was used to study DNA. A metal- organic compound tris (4,7- diphenylphenanthroline) ruthenium (Ⅱ) [Ru(DiP)32-] was chosen as a metal marker. The target DNA labeled with Ru (DiP)32- was electrophoresed in an agarose gel and then was analysed by PIXE. The DNA sample with the metal marker showed clearly Ru peaks in the PIXE spectra, while the control showed no Ru peak at all. This method can be used to study biology at the DNA level.     

Patatin启动子驱动的乙肝表面抗原基因在马铃薯中的表达

按常规分子克隆方法将马铃薯中高表达的ｐａｔａｔｉｎ启动子和乙肝表面抗原基因分别插入双元质粒ｐＢＩ１０１成为植物表达载体ｐＰＨＧ９,由前者驱动后者表达;通过农杆菌转化法将乙肝表面抗原基因导入马铃薯并获得再生植株;用ＥＬＩＳＡ方法检测经ＰＣＲ鉴定的转基因植株中乙肝表面抗原的含量,对照标准曲线得知转基因马铃薯植株中的乙肝表面朱含量高达１００￣１７４ｎｇ／ｍｇ可溶性蛋白。