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排序方式: 共有375条查询结果,搜索用时 29 毫秒
1.
Takashi Kanno Changmin Kim Daisuke Yamanaka Ken-ichi Ishibashi Hiroshi Tanaka Naohito Ohno Yoshiyuki Adachi 《International journal of molecular sciences》2021,22(4)
Because Japanese cedar pollen (JCP) contains beta-1,3-d-glucan (BG), there is concern that its lingering presence in the atmosphere, especially during its scattering period, may cause false positives in the factor-G-based Limulus amebocyte lysate (LAL) assay used to test for deep mycosis (i.e., G-test). Hence, we examined whether the LAL assay would react positively with substances contained in JCP by using the G-test to measure JCP particles and extracts. BG was purified from the JCP extract on a BG-specific affinity column, and the percentage extractability was measured using three different BG-specific quantitative methods. The G-test detected 0.4 pg BG in a single JCP particle and 10 fg from a single particle in the extract. The percentage extractability of JCP-derived BG was not significantly different among the three quantitative methods. As the JCP particles should technically have been removed during serum separation, they should be less likely to be a direct false-positive factor. However, given that the LAL-assay-positive substances in the JCP extract were not distinguishable by the three BG-specific quantitative methods, we conclude that they may cause the background to rise. Therefore, in Japan false positives arising from JCP contamination should be considered when testing patients for deep mycosis. 相似文献
2.
Hiromasa Tanno Emi Kanno Suzuna Sato Yu Asao Mizuki Shimono Shiho Kurosaka Yukari Oikawa Shinyo Ishi Miki Shoji Ko Sato Jun Kasamatsu Tomomitsu Miyasaka Hideki Yamamoto Keiko Ishii Yoshimichi Imai Masahiro Tachi Kazuyoshi Kawakami 《International journal of molecular sciences》2021,22(8)
Chronic infections are considered one of the most severe problems in skin wounds, and bacteria are present in over 90% of chronic wounds. Pseudomonas aeruginosa is frequently isolated from chronic wounds and is thought to be a cause of delayed wound healing. Invariant natural killer T (iNKT) cells, unique lymphocytes with a potent regulatory ability in various inflammatory responses, accelerate the wound healing process. In the present study, we investigated the contribution of iNKT cells in the host defense against P. aeruginosa inoculation at the wound sites. We analyzed the re-epithelialization, bacterial load, accumulation of leukocytes, and production of cytokines and antimicrobial peptides. In iNKT cell–deficient (Jα18KO) mice, re-epithelialization was significantly decreased, and the number of live colonies was significantly increased, when compared with those in wild-type (WT) mice on day 7. IL-17A, and IL-22 production was significantly lower in Jα18KO mice than in WT mice on day 5. Furthermore, the administration of α-galactosylceramide (α-GalCer), a specific activator of iNKT cells, led to enhanced host protection, as shown by reduced bacterial load, and to increased production of IL-22, IL-23, and S100A9 compared that of with WT mice. These results suggest that iNKT cells promote P. aeruginosa clearance during skin wound healing. 相似文献
3.
4.
Shahira Said Aly Tsuyoshi Imai Mohamed Salah Hassouna Diem-Mai Kim Nguyen Takaya Higuchi Ariyo Kanno Koichi Yamamoto Rinji Akada Masahiko Sekine 《International Journal of Hydrogen Energy》2018,43(10):5300-5313
The ability of Clostridium butyricum RAK25832 to use casamino acids as a nitrogen source was investigated. Strain RAK25832 showed the capacity to utilize different types of carbon sources. With glucose as a carbon source (10 g/L), the preferred final concentration of casamino acids was 26.67 g/L, with a cumulative hydrogen production, production rate, and yield of 2505 mL H2/L, 160 mL/h, and 1.81 mol H2/mol glucose, respectively. Eighteen metal elements were screened to identify the most important metals for biohydrogen production, and four elements were optimized. The optimal medium composition was MgCl2·6H2O (0.1 g/L), K2HPO4·3H2O (6.67 g/L), NaHCO3 (2.6 g/L), and FeCl2·4H2O (0.002 g/L). Vitamin supplementation of the medium showed no significant effect on hydrogen production. Under the optimized conditions, cumulative hydrogen production reached 3074 mL H2/L. This is the first study to demonstrate the use of casamino acids as a nitrogen source by C. butyricum. 相似文献
5.
6.
Tien-Hsiu Tsai Takumi Hamaguchi Ikuo Kanno 《Journal of Nuclear Science and Technology》2018,55(6):663-671
The filter-type ‘transXend’ detector is an energy-resolving X-ray detector consisting of an energy-integrating flat-panel detector and multiple filters. In our previous studies, we have shown the effectiveness of the transXend detector, but the filters used were not optimized. To obtain better performance, the filters, especially their thickness and material, should be considered. In this paper, we present a method that can preliminarily estimate filter performance by comparing their noise sensitivity before carrying out numerous experiments. Two kinds of filter sets, Cu–Sn and W–Ag, with various thicknesses were evaluated. The results suggest that to image a 20-cm-thick object with 120-kV X-rays, an unfiltered channel and a channel filtered with 0.5-mm-thick Sn or 0.4-mm-thick Ag may be the best combination. The optimal filter thickness will be smaller if the object is thinner and the tube voltage is lower. For applications that require a wide dynamic range of detector response, a channel with a W filter may be better than an unfiltered channel. To verify the calculation results, computed tomography imaging experiments with a 3-cm-diameter phantom were also performed, and the experimental results showed good agreement with the calculation results. 相似文献
7.
8.
Ryo Watada Makoto Ohsaki Yoshihiro Kanno 《Structural and Multidisciplinary Optimization》2011,43(4):459-471
Uniqueness and symmetry of solution are investigated for topology optimization of a symmetric continuum structure subjected
to symmetrically distributed loads. The structure is discretized into finite elements, and the compliance is minimized under
constraint on the structural volume. The design variables are the densities of materials of elements, and intermediate densities
are penalized to prevent convergence to a gray solution. A path of solution satisfying conditions for local optimality is
traced using the continuation method with respect to the penalization parameter. It is shown that the rate form of the solution
path can be formulated from the optimality conditions, and the uniqueness and bifurcation of the path are related to eigenvalues
and eigenvectors of the Jacobian of the governing equations. This way, local uniqueness and symmetry breaking process of the
solution are rigorously investigated through the bifurcation of a solution path. 相似文献
9.
Genki Kobayashi Shin‐ichi Nishimura Min‐Sik Park Ryoji Kanno Masatomo Yashima Takashi Ida Atsuo Yamada 《Advanced functional materials》2009,19(3):395-403
State‐of‐the‐art LiFePO4 technology has now opened the door for lithium ion batteries to take their place in large‐scale applications such as plug‐in hybrid vehicles. A high level of safety, significant cost reduction, and huge power generation are on the verge of being guaranteed for the most advanced energy storage system. The room‐temperature phase diagram is essential to understand the facile electrode reaction of LixFePO4 (0 < x < 1), but it has not been fully understood. Here, intermediate solid solution phases close to x = 0 and x = 1 have been isolated at room temperature. Size‐dependent modification of the phase diagram, as well as the systematic variation of lattice parameters inside the solid‐solution compositional domain closely related to the electrochemical redox potential, are demonstrated. These experimental results reveal that the excess capacity that has been observed above and below the two‐phase equilibrium potential is largely due to the bulk solid solution, and thus support the size‐dependent miscibility gap model. 相似文献
10.
M. Kope¢ A. Yamada G. Kobayashi S. Nishimura R. Kanno A. Mauger F. Gendron C.M. Julien 《Journal of power sources》2009
A series of LiMnyFe1−yPO4 samples have been prepared in the whole range 0 ≤ y ≤ 1. Chemical delithiation could be achieved to obtain MnyFe1−yPO4 in the range 0 ≤ y ≤ 0.8, keeping the same crystal phase (olivine structure, space group Pnma). The composition y = 0.8 is the limit where the delithiated phase is still crystallized, but abruptly suffers strains at the molecular scale evidenced by both optical spectroscopy and X-ray diffraction. The analysis of the magnetic properties shows that in all the samples the concentration of impurities is negligible. The concentration of polarons, either holes associated to Li vacancies in LiMnyFe1−yPO4 or electrons associated to the existence of Li left in the matrix of MnyFe1−yPO4, is found to be small (≤1%) in all the samples. For y ≤ 0.6, all the Mn3+ ions MnyFe1−yPO4 are in the high-spin state (S = 2). At larger manganese concentration, however, the Mn3+ ions in excess of the critical concentration yc = 0.6 undergo a transition to the low-spin state (S = 1). As a consequence, and in contrast with prior works, we find that Mn0.8Fe0.2PO4 has magnetic interactions that are much smaller, and no antiferromagnetic ordering in this compound is detected, at least above 20 K. Antiferromagnetic ordering that had been reported so far for MnyFe1−yPO4 at large y-composition might come from incomplete delithiation. The spin-transition of Mn3+ in concentration (y–yc) to the low-spin state is at the origin of the strain fields at the molecular scale that increase with y for y > 0.6, and ultimately prevents the full delithiation for y > 0.8. This result sheds light on the reason for the degradation of cathode properties in Mn-rich compounds of the heterosite–purpurite series, while the electrochemical properties are good in the range y ≤ 0.6 but only at slow rates, due to the very small hopping mobility of the small polaron. 相似文献