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Chia‐Wen Tsai Ai‐Hsuan Lin Tsung‐Shing Wang Kai‐Li Liu Haw‐Wen Chen Chong‐Kuei Lii 《Molecular nutrition & food research》2010,54(6):841-850
Understanding the molecular events underlying gene regulation by amino acids has attracted increasing attention. Here, we explored whether the mechanism by which methionine restriction affects the expression of the π class of glutathione S‐transferase (GSTP) is related to oxidative stress initiated by glutathione (GSH) depletion. Rat primary hepatocytes were cultured in an L‐15‐based medium in the absence or presence of 200 μM L ‐buthionine sulfoximine (BSO) or in a methionine‐restricted L‐15 medium supplemented with 20 μM L ‐methionine up to 72 h. BSO and methionine restriction time‐dependently induced GSTP mRNA and protein expression in a similar pattern accompanied by a decrease in the cellular GSH level. The phosphorylation of extracellular signal‐regulated kinase (ERK), but not of c‐Jun NH2‐terminal kinase and p38, was stimulated by methionine restriction and BSO. Electromobility gel shift assay showed that the DNA‐binding activity of nuclear activator protein‐1 (AP‐1) increased in cells exposed to methionine restriction or BSO. With the ERK inhibitor FR180204, AP‐1 activation and GSTP expression were abolished. Moreover, the induction of GSTP by methionine restriction and BSO was reversed by GSH monoethyl ester and N‐acetylcysteine. Our results suggest that methionine restriction up‐regulates GSTP gene expression, which appears to be initiated by the ERK‐AP‐1 signaling pathway through GSH depletion in rat hepatocytes. 相似文献
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Haw‐Yun Shin 《International Journal of Communication Systems》2009,22(3):237-255
Because of the limited bandwidth and restricted number of channels in the wireless access environment, the broadcast paradigm has become an important technique for accessing messages. Energy saving is an important issue in the mobile computing environment. In this paper, we propose a multichannel broadcast paradigm that constructs the broadcast channels according to the access frequency of each type of message in order to save energy in mobile devices (MDs). The pinwheel scheduling algorithm (PSA) presented in this paper is used to organize all types of messages in the broadcast channels in the most symmetrical distribution in order to reduce both the tuning time and the access time. The performance of the proposed mechanism is analyzed, and the improvement over existing methods is demonstrated numerically. The results show that the proposed mechanism is capable of improving both the tuning time and the access time due to the presence of skewness in the access distribution among the disseminated messages. When the number of message types increases, both the tuning time and the access time can be further improved as long as a small number of broadcast channels are added. Copyright © 2009 John Wiley & Sons, Ltd. 相似文献
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Technology learning can make a significant difference to renewable energy as a mitigation option in South Africa's electricity sector. This article considers scenarios implemented in a Markal energy model used for mitigation analysis. It outlines the empirical evidence that unit costs of renewable energy technologies decline, considers the theoretical background and how this can be implemented in modeling. Two scenarios are modelled, assuming 27% and 50% of renewable electricity by 2050, respectively. The results show a dramatic shift in the mitigation costs. In the less ambitious scenario, instead of imposing a cost of Rand 52/t CO2-eq (at 10% discount rate), reduced costs due to technology learning turn renewables into negative cost option. Our results show that technology learning flips the costs, saving R143. At higher penetration rate, the incremental costs added beyond the base case decline from R92 per ton to R3. Including assumptions about technology learning turns renewable from a higher-cost mitigation option to one close to zero. We conclude that a future world in which global investment in renewables drives down unit costs makes it a much more cost-effective and sustainable mitigation option in South Africa. 相似文献
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A new kind of terpolymer membrane was employed to separate a permselective water–alcohol mixture. This membrane was prepared via the copolymerization of acrylonitrile, sodium salt styrene sulfonic acid (SStSA), and hydroxyethyl methacrylate in dimethylsulfoxide with azobisisobutyronitrile as an initiator. The reaction mechanism, resultant structure, and polymer composition were confirmed by IR and elemental analysis. The effects of the feed composition on the polymer composition, mechanical properties, thermal properties, and degree of swelling were investigated. It was found that water permeated through the membrane preferentially in a water/alcohol system. The flux increased with the increase of SStSA, but the separation factor decreased drastically with higher SStSA. For a 50 wt % water–ethanol mixture, a flux of 0.65 kg/m2 h and a separation factor of 212 were obtained at 30°C when the membrane containing the highest SStSA content was used. The capacities of the metal ions absorbed by the membranes were investigated in the study. © 2003 Wiley Periodicals, Inc. J Appl Polym Sci 90: 244–250, 2003 相似文献
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Methanol-to-olefin (MTO) conversion on microporous silico-aluminophosphates is particularly well-suited for the application of molecular-level concepts to the development of well-defined supported catalysts. The active site of a typical MTO catalyst is a nm-size inorganic cage with an essential organic component. Opportunities for altering the selectivity of such catalysts include tailoring the organic component and modification of the cage with additional inorganic material through ship-in-a-bottle synthesis. The latter possibility has already been realized experimentally by the development of a useful and practical phosphate-modified catalyst. 相似文献
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An ordering of magnesium is observed in the as-synthesized MAPO-36 molecular sieve. Upon calcination, part of the magnesium is removed from the sample and part occupies extraframework positions thus rendering a random distribution of magnesium in the lattice. The shoulder observed in the31P spectrum of the calcined sample is assigned to P(3Al, 1Mg) sites in conflict with the earlier assignment to P-OH groups. The bridging hydroxyl groups located at these sites are detected in the1H MAS spectrum at 3.6 ppm. Further, MAPO-36 catalyzed acetone conversion to mesityloxide that cracked at elevated temperature to yield acetic acid and aliphatics. 相似文献
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Gavrilovic P. Timofeev F.N. Haw T. Williams J.E. 《Quantum Electronics, IEEE Journal of》1991,27(7):1859-1862
Data on long-cavity 100-μm-wide broad-stripe laser diodes that lase with a barrow single-lobed far-field pattern in continuous room-temperature operation are presented. Diodes with a cavity length of 1250 μm emit a power of 200 mW per facet into a 2.5° lobe (full width at half maximum). Short-cavity devices (cavity length of 350 μm) lase with a continuously increasing number of lateral modes right from threshold, and exhibit a far-field divergence that is over three times greater than that of 1250-μm diodes. Explanations for the effect of increasing cavity length on the field patterns of these devices are proposed, based on the measured increase in injected carrier diffusion length in long-cavity diodes and the influence of thermal waveguiding and mirror losses on intermodel discrimination 相似文献