杏鲍菇甲壳素对大鼠实验性脂肪肝的预防作用

为研究杏鲍菇甲壳素对脂肪肝的预防作用,采用给脂肪肝模型大鼠对其喂食不同浓度杏鲍菇甲壳素,以考来烯胺为阳性对照,饲养5周后观察其血清中丙氨酸转氨酶(alanine aminotransferase,ALT)、天冬氨酸转氨酶(aspartate aminotransferase,AST)、总胆固醇(total cholesterol,TC)、总甘油三酯(total triglycerides,TG)水平,肝脏组织中的TC、TG、丙二醛(malonaldehyde,MDA)水平、超氧化物歧化酶(superoxide dismutase,SOD)活性及肝组织病理学变化情况。结果表明:杏鲍菇甲壳素能极显著性(P0.01)地降低大鼠血清中ALT、AST、TC、TG水平,显著提高肝脏组织中SOD活性(P0.05)、降低MDA活性,减轻肝组织脂肪性样变,减少TC、TG在肝组织中积累,并呈现剂量效应,并值得注意的是,与考来烯胺组相比,杏鲍菇甲壳素高剂量组的预防效果与之相近,无差异性(P0.05)。结论:杏鲍菇甲壳素具有预防脂肪肝形成的作用。

Preventive Effect of Chitin from Pleurotus eryngii on Fatty Liver in Rats

An experimental rat model of fatty liver was constructed to explore the preventive effect of chitin from
Pleurotus eryngii on fatty liver. The rats were fed chitin at various concentration levels while cholestyramine was used
as a positive control. After 5 weeks, the levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST),
total cholesterol (TC) and total triglycerides (TG) in serum and the levels of malonaldehyde (MDA) and superoxide
dismutase (SOD) in liver were detected and pathological changes in liver were examined. Results showed the levels
of ALT, AST, TC and TG in serum and the levels of TC, TG and MDA in liver significantly decreased (P < 0.01), and
SOD activity significantly increased (P < 0.05) after administration with P. eryngii chitin for 5 weeks. Moreover, fatty
degeneration in liver tissue was alleviated, and the levels of TC and TG were reduced in liver tissue. Furthermore, the
hepatoprotective activity of P. eryngii chitin (PC) was dose dependent. Notably, the preventive effect of high-dose PC
was similar to that of cholestyramine (P < 0.05). All these results indicated that P. eryngii chitin has the potential to
prevent fatty liver disease in rats.