免疫亲和柱净化-光化学柱后衍生高效液相色谱 荧光法测定粮食中黄曲霉毒素

建立了粮食中黄曲霉毒素B1、B2、G1、G2的免疫亲和柱净化-光化学柱后衍生高效液相色谱荧光检测法。样品经甲醇-水提取,免疫亲和柱净化,高效液相色谱分离,光化学柱后衍生,荧光检测器测定。结果表明,黄曲霉毒素B1、B2、G1、G2的检出限分别为0.50、0.25、0.50、0.25μg/kg,回收率为67.2%～91.7%,RSD小于10%。该方法快速、准确、灵敏度高、重现性好,能满足我国对粮食中黄曲霉毒素限量的检测要求。

Determination of aflatoxin in cereal by immune-affinity column clean-up and HPLC-FLD with post-column photochemical derivation

A method for determination of aflatoxin B1,B2,G1 and G2 by immune-affinity column clean-up and HPLC with post column photochemical derivation was developed.Samples were extracted with methanol-water solution and cleaned up by immune-affinity columns.Separation of aflatoxins was conducted by HPLC and the determination was carried out by fluorescence detector after photochemical derivation.The results showed that the limits of detection of aflatoxin B1,B2,G1 and G2 were 0.5,0.25,0.5,0.25 μg/kg respectively,and the recovery rates were from 67.2% to 91.7%,RSD was below 10%.The method is quick,precise,high-sensitive and good-repeatable,which is suitable for the determination of aflatoxin in cereals in China.