酸、碱溶液对脱脂蚕蛹组织结构的影响

在100℃下将脱脂蛹分别置于清水、盐酸溶液(1%wt)和氢氧化钠溶液(1%wt)中浸泡处理20min,采用电子光学显微分析技术,观察处理后脱脂蛹的解剖结构,并对浸泡液进行了定性分析。结果表明:(1)清水、盐酸溶液、氢氧化钠溶液浸泡,均引起蛋白质、甲壳素膨胀,氢氧化钠溶液对蚕蛹的蛋白层与甲壳层的层间有劈开作用;(2)盐酸溶液作用下,蛹蛋白的分解产物是氨基酸;蛹蛋白在碱溶液中溶解显著,同时,伴有部分蛋白质的分解,其分解产物是氨基酸,而氨基酸进一步分解可释放出氨。

The influence of acid and alkaline solutions on tissue of defatted silkworm chrysalis

Defatted silkworm chrysalis was soaked in water, hydrochloric acid solution(1%wt) and sodium hydroxide solution (1%wt) respectively at 100 centigrade for 20 minutes. After the treatment, the structure of defatted silkworm chrysalis was observed by electron optical microscope analysis and the soaking solution was determined qualitatively. The results showed that: (1)soaked in water, hydrochloric acid and sodium hydroxide solution, the protein and chitin expanded; sodium hydroxide solution made the interlayer of protein and chitin cleaved. (2)in hydrochloric acid solution, the decomposed production of protein was amino acid, while in sodium hydroxide solution, protein dissolved markedly and certain amount of protein was decomposed to amino acid that could be broken down into ammonia further.