大豆对SMV1号株系的抗性遗传分析及抗病基因的SSR标记

利用杂交组合合丰25（S）×东农93-046（R）的F1、F2和F2∶3群体进行了抗病性鉴定,结果表明：F1在接种后表现抗病,F2群体分离比例为3（抗）∶1（感）,对F2衍生的F2∶3家系接种鉴定,纯合抗病家系、抗感分离家系和纯合感病家系的比率符合1∶2∶1,表明东农93-046对SMV1号株系的抗性由一对显性基因（RSMV1）控制。并利用东农93-046（R）×Conrad（S）的正反交组合F2进行抗性鉴定,结果正反交后代均表现为3∶1的分离比例,无细胞质效应,进一步证明了东农93-046是受一对基因控制的抗性种质。经改良的分离群体组群分析法研究发现,东农93-046抗SMV1的位点（RSMV1）位于F连锁群上,与SSR标记的连锁顺序为HSP176、Satt114、RSMV1、Satt510、Sct_033、Satt334、Satt362,标记与RSMV1之间的遗传距离分别为10.9cM、4.3cM、6.6cM、11.7cM、18.0cM和35.3cM。根据标记HSP176选择基因型纯合和杂合的抗病植株,其符合率分别达到100.0%和94.5%。

Genetic analysis of resistance and identification of SSR marker linked to the resistance gene of soybean（Glycine max L.Merr.） to SMV1

Soybean resistance to SMV1 was investigated by artificial inoculation.F1,F2 and F2∶ 3 populations from Hefeng25（S） × Dongnong93-046（R） were used as materials.Result showed that F1 had the resistance.Segregation ratio of 225 F2 populations was 3（resistant and necrosis）∶ 1（susceptible）.The ratio of F2∶ 3 families were 1（resistant）∶ 2（heterozygous）∶ 1（susceptible）.Results also showed that Dongnong93-046 had a single dominant resistant gene to SMV1.Investigation of reciprocal crosses between Dongnong93-046（R） and Conrad（S） proved that resistance to susceptibility ratio was 1∶ 1.No cytoplasmic effect was found which indicated that Dongnong93-046 was a highly resistant accession,and a single dominant gene conferred plant resistance to SMV1.Modified bulked segregation analysis（BSA） demonstrated that 6 SSR markers in F linkage group were closely linked to RSMV1 in F2 mapping population from Hefeng25 × Dongnong93-046.The orientation of RSMV1 and 6 SSR markers was determined as HSP176（10.9cM）,Satt114（4.3cM）,RSMV1（0.0cM）,Satt510（6.6cM）,Sct_033（11.7cM）,Satt334（18.0cM）,Satt362（35.3cM）.Coincidence rates of selecting homozygous and heterozygous resistant plants were 100% and 94.5% respectively using HSP176 marker.