| 龟苓膏中绿原酸含量测定及衰减跟踪监测 |
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| 引用本文： | 李亚,梁剑锋.龟苓膏中绿原酸含量测定及衰减跟踪监测[J].现代食品科技,2011(9):1163-1165. |
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| 作者姓名： | 李亚 梁剑锋 |
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| 作者单位： | 梧州市产品质量监督检验所; |
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| 摘 要： | 提出了高效液相色谱法测定龟苓膏中绿原酸含量的方法.样品经50％甲醇超声提取,以Gemini-C18色谱柱(250 mmnx4.60mm,5μm)为分离柱,以甲醇-0.4％瞵酸溶液(22:78)为流动相,用紫外检测器于327nm处检测,外标法定量.方法检出限为0.05μg/mL,加标回收率为97％,相对标准偏差(n=6)...
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| 关 键 词： | 高效液相色谱 绿原酸 衰减跟踪监测 |
Determination of Chlorogenic Acid in Guiling Jelly and Content Attenuation Tracking and Monitoring |
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| LI Ya,LIANG Jian-feng.Determination of Chlorogenic Acid in Guiling Jelly and Content Attenuation Tracking and Monitoring[J].Modern Food Science & Technology,2011(9):1163-1165. |
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| Authors: | LI Ya LIANG Jian-feng |
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| Affiliation: | LI Ya,LIANG Jian-feng(Wuzhou Institute of Product Quality Supervision and Inspection,Wuzhou 543002,China) |
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| Abstract: | A method for the determination of chlorogenic acid in Guiling Jelly was proposed.The sample was extracted by 50% methanol with ultrasonic wave.The extract was separated on a Gemini C18 column(250 mm×4.6 mm,5 μm) with methanol-0.4% phosphoric acid(22:78) as the mobile phase,and determined by UV detection at 327 nm.An external standard method was adopted.The determination limit of the method was 0.05 μg/mL,the recovery adding standard was 97%,and the relative standard deviation(n=6) was 2.9%.The content attenuation tracking and monitoring showed that the attenuation law of chlorogenic acid in Guiling jelly was influeced by several factors such as the storage time,ambient temperature and packaging. |
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| Keywords: | HPLC chlorogenic acid attenuation tracking and monitoring |
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