高效液相色谱法同时测定小麦粉及其制品中过氧化苯甲酰和苯甲酸

目的建立同时测定小麦粉及其制品中过氧化苯甲酰和苯甲酸的高效液相色谱检测方法。方法样品中过氧化苯甲酰和苯甲酸用无水乙醇进行提取,采用色谱柱为Symmetry C18(150 mm×4.6 mm,5μm),以甲醇-0.1%磷酸水溶液梯度洗脱,检测波长235 nm。结果过氧化苯甲酰、苯甲酸与杂质基线分离,过氧化苯甲酰和苯甲酸在0.10~20 mg/L浓度范围内线性良好,相关系数r均在0.999 9以上。过氧化苯甲酰和苯甲酸回收率分别为95.2%~99.5%和95.3%~105%,方法检出限分别为0.10和0.08 mg/kg,定量限分别为0.30和0.24 mg/kg。结论本方法操作简便、灵敏度高、结果准确,适用于小麦粉及其制品中过氧化苯甲酰和苯甲酸同时分析。

Simultaneous determination of benzoyl peroxide and benzoic acid in wheat flour and its products by high performance liquid chromatography

To develop an analytical method for the simultaneous separation and determination of residual benzoyl peroxide (BPO) and benzoic acid (BA) in flour and wheat products by HPLC.Methods BPO and BA were extracted by ethanol, and separation and determination were carried out on Symmetry C₁₈ column using a gradient mobile phase by HPLC at 235 nm. Results BPO and BA showed good linearity (r>0.999 9) in the range of 0.10-20 mg/L. For BPO and BA, the recoveries were 95.2%-99.5% and 95.3%-106%, respectively; the limits of detection (LOD) were 0.10 and 0.08 mg/kg; the limits of quantification (LOQ) were 0.30 and 0.24 mg/kg.Conclusion The method is simple and reliable for the determination of BPO and BA in wheat flour and its products.