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蔬菜抗氧化能力及与酚酸和总黄酮相关性研究
引用本文:周佳,阮征,江波,欧阳崇学,印遇龙.蔬菜抗氧化能力及与酚酸和总黄酮相关性研究[J].食品与机械,2012,28(3):139-143.
作者姓名:周佳  阮征  江波  欧阳崇学  印遇龙
作者单位:南昌大学食品科学与技术国家重点实验室,江西南昌 330047;南昌大学生命科学与食品工程学院,江西南昌 330031
基金项目:南昌大学“赣江学者奖励计划”项目
摘    要:分析常见蔬菜总抗氧化活性,探讨蔬菜抗氧化能力与酚酸、总黄酮的相关性。采用ABTS、DPPH与FRAP法测定10种常见蔬菜的抗氧化能力,采用Folin-Ciocalteu法测总酚酸,硝酸铝-亚硝酸钠-氢氧化钠法测总黄酮,采用matlab软件进行拟合抗氧化与酚酸、总黄酮的函数方程。结果表明:抗氧化活性与主要抗氧化物质(酚酸和黄酮)的函数关系为Z=-137.743 2-0.918 2 X2+0.211 2Y2-0.422 9 XY+52.843 5 X+2.229 9Y。当总黄酮在6~26mg/100g时,总黄酮、酚酸的量对总抗氧化活性有促进作用;而在27~54mg/100g范围内,总黄酮和酚酸的含量对总抗氧化能力具有一定的抑制作用。

关 键 词:蔬菜  抗氧化  ABTS  DPPH  FRAP  黄酮  酚酸

Analysis of antioxidant capacity of vegetables and correlation of phenolic acid and flavonoids and antioxidant capacity
ZHOU Jia , RUAN Zheng , JIANG Bo , OUYANG Chong-xue , YIN Yu-long.Analysis of antioxidant capacity of vegetables and correlation of phenolic acid and flavonoids and antioxidant capacity[J].Food and Machinery,2012,28(3):139-143.
Authors:ZHOU Jia  RUAN Zheng  JIANG Bo  OUYANG Chong-xue  YIN Yu-long
Affiliation:1,2 (1.State Key Laboratory of Food Science and Technology,Nanchang University,Nanchang,Jiangxi 330047,China;2.College of Life Science and Food Engineering,Nanchang University,Nanchang,Jiangxi 330031,China)
Abstract:The paper was to evaluate the correlation of phenolic acid,flavonoids and antioxidant capacity.ABTS,DPPH and FRAP methods were used to determinate antioxidant capacity of 10 kinds of vegetables.The correlation of phenolic acid,flavonoids and antioxidant capacity was analysed with matlab.Results: The function of antioxidant(phenolic acid and flavonoids) and antioxidant capacity was as following:Z =-137.743 2-0.918 2X2 + 0.211 2Y2-0.422 9XY + 52.843 5X + 2.229 9Y.There was a positive correlation between the content phenolic acid and total antioxidant activity.The interaction between phenolic acid and flavonoid had inhibitory effect on antioxidant capacity.
Keywords:vegetables  antioxidation  ABTS  DPPH  FRAP  phenolic acid  flavonoids
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