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液相色谱-串联质谱法检测动物组织中阿布拉霉素
引用本文:赵凤娟,岳振峰,肖陈贵,叶刚,吴卫东,胡晓苑.液相色谱-串联质谱法检测动物组织中阿布拉霉素[J].食品安全质量检测技术,2013,4(1):159-164.
作者姓名:赵凤娟  岳振峰  肖陈贵  叶刚  吴卫东  胡晓苑
作者单位:深圳出入境检验检疫局食品检验检疫技术中心, 深圳市食品安全检测技术研发重点实验室深圳 518045,深圳出入境检验检疫局食品检验检疫技术中心, 深圳市食品安全检测技术研发重点实验室深圳 518045,深圳出入境检验检疫局食品检验检疫技术中心, 深圳市食品安全检测技术研发重点实验室深圳 518045,深圳出入境检验检疫局食品检验检疫技术中心, 深圳市食品安全检测技术研发重点实验室深圳 518045,深圳出入境检验检疫局食品检验检疫技术中心, 深圳市食品安全检测技术研发重点实验室深圳 518045,深圳出入境检验检疫局食品检验检疫技术中心, 深圳市食品安全检测技术研发重点实验室深圳 518045
摘    要:目的 建立动物源食品中阿布拉霉素的LC-MS/MS检测方法。方法 待测样品中的阿布拉霉素用5%的三氯乙酸溶液提取, 经MCX固相萃取柱净化、浓缩、定容后采用液相色谱-串联质谱法在正离子模式下检测。结果 该方法在0~5 mg/L范围内有良好的线性关系, 相关系数r>0.995, 在不同基质中, 不同的添加水平下该方法的平均回收率范围在80.9%~101%, 相对标准偏差为1.17%~12.60%; 定量下限为0.030 mg/kg。结论 此方法灵敏度高、准确、重现性好, 适用于动物组织中阿布拉霉素残留量的检测。

关 键 词:阿布拉霉素    高压液相色谱-串联质谱    固相萃取    动物组织
收稿时间:2012/11/30 0:00:00
修稿时间:2013/1/17 0:00:00

Determination of apramycin residues in animal tissues by high pressure liquid chromatography-tandem mass spectrometry
ZHAO Feng-Juan,YUE Zhen-Feng,XIAO Chen-Gui,YE Gang,WU Wei-Dong and HU Xiao-Yuan.Determination of apramycin residues in animal tissues by high pressure liquid chromatography-tandem mass spectrometry[J].Food Safety and Quality Detection Technology,2013,4(1):159-164.
Authors:ZHAO Feng-Juan  YUE Zhen-Feng  XIAO Chen-Gui  YE Gang  WU Wei-Dong and HU Xiao-Yuan
Institution:Shenzhen Key Laboratory of Detection Technology RD on Food Safety, Food Inspection and Quarantine Center, Shenzhen Entry-Exit Inspection and Quarantine Bureau,Shenzhen Key Laboratory of Detection Technology RD on Food Safety, Food Inspection and Quarantine Center, Shenzhen Entry-Exit Inspection and Quarantine Bureau,Shenzhen Key Laboratory of Detection Technology RD on Food Safety, Food Inspection and Quarantine Center, Shenzhen Entry-Exit Inspection and Quarantine Bureau,Shenzhen Key Laboratory of Detection Technology RD on Food Safety, Food Inspection and Quarantine Center, Shenzhen Entry-Exit Inspection and Quarantine Bureau,Shenzhen Key Laboratory of Detection Technology RD on Food Safety, Food Inspection and Quarantine Center, Shenzhen Entry-Exit Inspection and Quarantine Bureau,Shenzhen Key Laboratory of Detection Technology RD on Food Safety, Food Inspection and Quarantine Center, Shenzhen Entry-Exit Inspection and Quarantine Bureau
Abstract:Objective To establish a method for the determination of apramycin in animal tissues by LC-MS/MS. Methods The residues of apramycin in the test samples were extracted with 5% trichloroacetic acid solution. After being cleaned up with SPE column, concentrated and reconstituted, the residues were detected by liquid chromatography-mass spectrometry under multiple reaction monitoring (MRM) mode via positive-ion mode. Results The method showed a good linearity over the range of 0~5 mg/L for apramycin with r>0.995. The average recoveries were 80.9%~101% at three or four spiked levels in animal tissues, and the relative standard deviations were 1.17%~12.6%. The limit of quantitation was 0.030 mg/kg. Conclusion This method is highly sensitive, accurate and reproducible, and it is suitable for the detection of apramycin in animal tissues.
Keywords:apramycin  high pressure liquid chromatography-tandem mass spectrometry  solid phase extraction  animal tissues
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