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酪蛋白酶解物中抗菌肽的抑菌性及稳定性研究 总被引:2,自引:0,他引:2
对酪蛋白胰蛋白酶酶解物中抗菌肽的抗菌性及稳定性进行研究。实验通过以大肠杆菌、金黄色葡萄球菌、枯草芽孢杆菌和蜡样芽胞杆菌为指示菌,验证了抗菌肽的抑菌性,并以大肠杆菌为指示菌,研究了酶解物中抗菌肽在不同温度、不同pH值、不同储藏时间条件下的抑菌稳定性。结果表明:此抗菌肽能有效抑制大肠杆菌、金黄色葡萄球菌、枯草芽孢杆菌和蜡样芽胞杆菌的生长,具有广谱抗菌活性;碱性及中性条件下,此抗菌肽对大肠杆菌抑菌活性稳定,酸性条件下,抑菌活性随pH值下降而增强;此抗菌肽冻干样品在室温下可长期储藏。 相似文献
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天然食品防腐剂--抗菌肽 总被引:9,自引:0,他引:9
TheNaturalFoodPreservartive-AntimicrobialPeptideLuiYiNingZhengxiang具有抗菌作用的多肽简称抗菌肽,它们在自然界中广泛存在。已确定一些抗菌肪的基因编码,可以利用基因工程使生产简单化,加宽抗菌谱,提高产量[1,2,3]。本文介绍了来自植物、昆虫、微生物和脊椎动物的抗菌肽,比较了它们的分子结构和作用方式,并讨论了应用前景。1微生物中的抗菌肽1.l细菌素和细菌素类似物工.1.五小菌素小菌素主要由肠杆菌科细菌产生,分子量通常低于SKDa。它们结构紧密、热稳定性好,对链霉蛋白酶或枯草溶菌素有抗性。所有的小菌素都… 相似文献
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乳酪蛋白源抗菌肽——结构、功能及应用 总被引:2,自引:0,他引:2
基因编码的抗菌肽广泛分布于昆虫、植物、动物及人体内,是天然免疫防御系统的重要组成部分.然而,抗菌肽亦能通过食物蛋白水解产生,这些食物蛋白源抗菌肽可视为"营养免疫"的重要组成部分.酪蛋白是乳中最主要的蛋白质,除了提供氨基酸和能量的营养功能外,酪蛋白氨基酸序列中还存在大量的抗菌肽片段,当用适当的蛋白酶进行体外水解或在胃肠道消化过程中以及食品加工过程中,它们的活性就被释放出来.体外实验表明,这些抗菌肽片段具有广谱抗菌活性.然而,用动物模型或人类临床试验研究这些抗菌肽体内活性的报道却很少,且这些抗菌肽的抗菌机制目前还不清楚,乳酪蛋白源抗菌肽的体内生物学效应还需更多的试验加以评价和证实.本文对乳酪蛋白来源的抗菌肽的序列结构、获取方法、抗菌作用及其应用前景做一阐述. 相似文献
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壳聚糖抗菌抗氧化活性及其在食品保鲜中应用 总被引:1,自引:0,他引:1
壳聚糖因其可生物可降解、生物相容好、无毒和具有天然抗菌抗氧化活性,使其成为食品保鲜与防腐研究的热点。本文在壳聚糖结构与抗菌抗氧化活性的基础上,重点概述了近年来壳聚糖在食品保鲜中的应用。 相似文献
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Thibaut Thery Kieran M. Lynch Elke K. Arendt 《Comprehensive Reviews in Food Science and Food Safety》2019,18(5):1327-1360
A large range of ingredients for food and food products are subject to fungal contamination, which is a major cause of destruction of crops, exposure of animals and humans to invasive mycotoxins, and food spoilage. The resistance of fungal species to common preservation methods highlights the necessity of new ways to increase the shelf life of raw material for food and food products. Antimicrobial peptides and proteins (AMPs) are essential members of the immune system of most living organisms. Due to their broad range of activity and their stability to commonly used food processes, they represent promising alternatives to traditional preservatives. However, despite the growing number of reports of potential food applications of these AMPs, the number of approved peptides is low. Poor solubility, toxicity, and a time‐consuming extraction are hurdles that limit their application in food products. Thanks to a deep understanding of the key determinants of their activity, the development of optimized synthetic peptides has reduced these drawbacks. This review presents natural and synthetic antifungal peptides/proteins (AFPs), effective against food‐related fungi, with particular emphasis on AFPs from plant sources. The design of novel antifungal peptides via key elements of antifungal activity is also reviewed. The potential applications of natural and synthetic AFPs as novel antifungal food preservatives are finally discussed. 相似文献
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食品安全问题一直为人们所关注,其中微生物污染是导致食品腐败变质的主要原因。抗菌肽来源广泛,抑菌谱广,杀菌速度快,可以较好地控制食品中腐败微生物的滋生;对pH值、蛋白酶和热处理有较好的稳定性,在成分复杂的食品体系中有一定的适用性;由于其抑菌机理特殊,不易产生耐药性;部分具有抗氧化和提高免疫力等多种功能,对人体具有一定的保健作用。抗菌肽的以上特点满足了人们对新型防腐保鲜剂的多种需求,有望在食品工业上发挥重大作用。因此,在抗菌肽的生物学功能以及抗菌肽保鲜机理简单介绍的基础上,对抗菌肽应用于不同食品中的研究现况进行详细综述,最后对抗菌肽在食品中应用目前存在的问题和发展方向提供了思考。 相似文献
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Antimicrobial peptides (AMPs) are a group of molecules that has gained interest in research due to their important role in the inhibition of bacterial agents. Their structure shows heterogeneity among the different types of AMPs. However, most of them stand out for their cationic nature and amphipathic character that allows them to interact with membrane components of bacterial cells. Some features such as high selectivity and thermostability have attracted the interest of the food industry toward the application of AMPs in food preservation due to their ability to inhibit foodborne pathogens and spoilage microorganisms. Despite this, there is a limited number of AMPs used as food preservatives nowadays. Therefore, this review aims to offer an overview of AMPs, challenges, and potential applications in the food industry. 相似文献
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水分活度(Aw)与食品防腐 总被引:14,自引:0,他引:14
水分活度是食品中水的蒸气压与同温下纯水的蒸气压之比,用符号Aw表示。近来的研究证明,它与食品的保鲜质量关系密切。食品中的水活性可以影响食品中微生物的繁殖,代谢,抗性及生存等。控制食品水活度,再辅以其它保鲜措施,可以有效提高食品的货架期。 相似文献
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植物多酚的抑菌活性及其在食品保鲜中的应用 总被引:1,自引:0,他引:1
植物多酚是一种植物次生代谢物, 其结构多样, 具有多种生物活性。植物多酚的抑菌活性是其重要活性之一, 并在食品中得到了广泛的应用。本文主要综述了植物多酚的组成、性质, 抑菌机制以及在食品保鲜中的应用。 相似文献
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Ortega-Rivas E 《Critical reviews in food science and nutrition》2007,47(2):161-173
Food functionality can be related to nutrient and non-nutrient compounds. Some foods are fortified with various nutrients whereas others are manipulated using their physicochemical properties in order to make them "functional." Texture, color, taste, odor, and other physicochemical properties may affect the overall quality and, hence, acceptance of food products. Since food safety is also an important factor to be considered in food processing, preservation methods are selected taking into account the best compromise between microbial inactivation and preservation of quality attributes. Instrumental and sensory methods, as well as a match between them, are studied and perfected in order to verify how well a specific type of food has been processed to make it "functional" in sensory terms. 相似文献
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在过去的几十年中,由于人们对抗生素药物的过量使用,导致抗微生物耐药性(Antimicrobial Resistance, AMR)的产生,这促使人们开始寻找能够替代传统抗生素的潜在替代品,从而降低AMR的上升。目前,研究人员在对抗微生物的生物化学分子中开始持续关注具有广谱抗菌活性的抗菌肽(Antimicrobial peptide , AMPs)。AMPs是一种能够抑制细菌、真菌、病毒、寄生虫、肿瘤细胞等的生物分子。AMPs代表宿主抵御病原体的第一道防线,是不同生物体先天免疫系统的重要组成部分。并且具有快速杀伤活性、低毒性和细胞选择性等优点,使其在医药、食品、畜牧业、农业等领域均具有良好的应用前景。在这篇综述中,我们概述了AMR 的发展、AMPs的来源、结构、生物活性和作用机制,并讨论了AMPs转化为临床应用所面临的挑战。我们旨在总结AMPs结构和作用机制的最新证据,并提供其微生物活性的详细证据。为人们进一步深入研究AMPs的抗微生物作用机制提供借鉴与参考。 相似文献
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Nonthermal Food Processing Alternatives and Their Effects on Taste and Flavor Compounds of Beverages
Enrique Ortega-Rivas Iván Salmerón-Ochoa 《Critical reviews in food science and nutrition》2014,54(2):190-207
Food drinks are normally processed to increase their shelf-life and facilitate distribution before consumption. Thermal pasteurization is quite efficient in preventing microbial spoilage of many types of beverages, but the applied heat may also cause undesirable biochemical and nutritious changes that may affect sensory attributes of the final product. Alternative methods of pasteurization that do not include direct heat have been investigated in order to obtain products safe for consumption, but with sensory attributes maintained as unchanged as possible. Food scientists interested in nonthermal food preservation technologies have claimed that such methods of preserving foods are equally efficient in microbial inactivation as compared with conventional thermal means of food processing. Researchers in the nonthermal food preservation area also affirm that alternative preservation technologies will not affect, as much as thermal processes, nutritional and sensory attributes of processed foods. This article reviews research in nonthermal food preservation, focusing on effects of processing of food drinks such as fruit juices and dairy products. Analytical techniques used to identify volatile flavor-aroma compounds will be reviewed and comparative effects for both thermal and nonthermal preservation technologies will be discussed. 相似文献