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在单因素试验的基础上,选择超声时间、超声温度和料液比为自变量,柚核中柠檬苦素类物质的提取率为响应值,进行Box-Benhnken中心组合试验设计,运用响应面分析法优化梅州金柚核柠檬苦素类物质的提取工艺。结果表明,梅州金柚核柠檬苦素类物质超声提取最佳工艺为提取温度50℃,料液比1∶20(g/m L),超声时间20 min。此工艺下柠檬苦素类物质提取率为0.491 mg/g(RSD=1.13%,n=3),与理论值0.487 mg/g偏差很小。 相似文献
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利用高效液相色谱法对常山胡柚皮中柠檬苦素的含量进行测定,比较了有机溶剂提取、水提取、超声波辅助提取条件下,常山胡柚皮中柠檬苦素的提取得率。结果表明:用乙醇提取时,提取温度50℃,料液比1∶20(g/m L),提取时间60 min,柠檬苦素得率为2.97 mg/g;用水提取时,提取温度80℃,料液比1∶25(g/m L),提取时间90 min,柠檬苦素得率为2.42 mg/g。超声辅助提取时,以乙醇作为提取溶剂,柠檬苦素得率为3.32 mg/g;以水作为提取溶剂,柠檬苦素得率为2.87 mg/g。 相似文献
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超声波辅助提取金橘柠檬苦素工艺研究 总被引:3,自引:0,他引:3
目的:研究金橘柠檬苦素提取工艺条件.方法:以金橘为原料,采用二次回归通用旋转组合试验设计,探讨超声波辅助提取金橘柠檬苦素的最佳工艺参数.结果:确定最优超声波处理时间20min,处理功率440W,浸提温度60℃,液料比30:1,浸提60min,柠檬苦素超声波辅助萃取得率达(3.26±0.03)‰,RSD=0.86%(n=5).结论:试验中所选参试因子对超声波辅助萃取金橘柠檬苦素效率影响显著,其影响次序为超声波处理时间>超声波处理功率>漫提温度>液料比. 相似文献
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Microwave processing has been used for the extraction of antioxidants from mandarin peels using deionised water as the solvent. Optimum extraction conditions under constant heat load per unit mass were as follows: microwave power, 400 W; extraction time, 3 min; solid to solvent ratio, 1:2; and extraction temperature below 135 °C. These conditions did not change the colour of the extract significantly under neutral (pH 4.5-4.9) and acidic conditions (pH 1.5-2.5). The amounts of antioxidants, which were extracted when the heat load per unit mass was held constant, were not significantly affected by the solid to solvent ratio used, so the maximum solid to solvent ratio of 1:2 used here is recommended to minimise the amount of solvent used. The colour of the extract was more greatly affected by the extraction temperature than by the solid to solvent ratio. The total phenolic contents of mandarin peels were evaluated using the Folin-Ciocalteu assay (FCR) and the trends were compared with the results of the Oxygen Absorbance Radical Capacity (ORAC) assays. The presence of Maillard rections and Maillard by-products were also investigated at higher extraction temperatures and analysed by the High Performance Liquid Chromatography (HPLC) method. The results demonstrated that microwave extraction (MWE) is a fast and reliable method for phenolic compound extraction from citrus marc with the minimum use of solvent. 相似文献
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In this study, optimum conditions for the extraction of black carrot anthocyanins were determined by response surface methodology. Central composite design of extraction factors (pH 2.5–6.5, temperature 4–72 °C, solvent/solid ratio 5:1–25:1 v/w, ethanol/water ratio 0:100–100:0?v/v) was generated as two replicates. Total phenolic content, total monomeric anthocyanin content, polymeric color, total antioxidant activity, and anthocyanin composition determined by high-performance liquid chromatography were used as responses. Except for color analysis, higher temperature, solid/solvent ratio, and ethanol concentration were observed to increase the extraction yield. However, polymeric color results were found to have minimum values at lower pH and solid/solvent ratio, lower or moderate temperature, and higher ethanol concentration. Optimum extraction conditions were found as follows: 50 °C, pH 3.5, solvent solid ratio 10:1 (v/w), and ethanol/water ratio 75:25 (v/v) when all responses were considered. The validation of the optimum conditions for black carrot extraction was performed at specified values. 相似文献
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桔梗中桔梗总皂苷和桔梗皂苷D的超声波提取工艺 总被引:1,自引:0,他引:1
分别以水和甲醇为提取溶剂,研究超声波法提取桔梗中桔梗总皂苷和桔梗皂苷D的工艺条件。采用正交试验法,考察提取次数(A)、料液比(B)、提取时间(C)、超声功率(D)对桔梗总皂苷和桔梗皂苷D提取率的影响。以水为提取溶剂时,超声提取桔梗总皂苷的影响顺序:提取次数>超声功率>料液比>提取时间,最优组合A3B3C3D2;提取桔梗皂苷D的影响顺序:超声功率>料液比>提取次数>提取时间,最优组合A2B3C1D1。以甲醇为提取溶剂时,超声提取桔梗总皂苷的影响顺序:提取次数>超声功率>提取时间>料液比,最优组合A3B2C1D3;提取桔梗皂苷D的影响顺序:提取时间>提取次数>料液比>超声功率,最优组合A2B1C2D3。 相似文献
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对高温高压条件下以水作为提取剂提取桂皮胶的工艺进行研究。在粉碎粒度、pH、料液比、提取温度、提取时间5个因素的单因素实验基础上,采用响应面分析法建立多元统计回归模型,考察桂皮胶提取过程中的四个主要影响因子即pH、料液比、提取温度、提取时间及其交互作用对提取量的影响。结果表明高压浸提法提取桂皮胶最佳工艺条件为pH5.0,料液比1:44(g/mL),提取温度125℃,提取时间31 min,在此最佳工艺条件下进行验证得到桂皮胶提取量为186.5 mg/g,与预测值187.1219 mg/g接近。桂皮胶提取物中总糖含量达到96.06%,采用此模型对桂皮胶提取量进行优化具有可行性。 相似文献
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旨在建立用反相高效液相色谱(RP-HPLC)法同时检测小麦胚中谷胱甘肽(glutathione)还原型(G-SH)和氧化型(G-S-S-G)含量的方法.为了更高效地提取谷胱甘肽,在水作溶剂条件下,考察了不同浸泡时间、超声时间、料液比、超声温度、溶剂pH和沉淀剂比例对谷胱甘肽提取效率的影响.并在2种色谱柱上,考察了5种流动相下谷胱甘肽含量测定结果.最终确定最适提取条件为:提取溶剂pH4.0,料液比1∶30,浸泡1h后在0℃下超声10min,沉淀剂比例为1∶2.0;色谱条件为:Fusion-RP柱(150×4.6mm,4μm,8nm);流动相:水(0.08%辛烷磺酸钠+0.24%磷酸二氢钠,以磷酸调pH2.5)-乙腈(体积比为82∶8);检测波长为210nm;流速为1.0mL·min-1;柱温为25℃.测定了19份不同来源麦胚中谷胱甘肽的含量,发现谷胱甘肽含量差异显著,最高达180.71mg·(100g)-1. 相似文献
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为了开发利用浙江省临安市太阳镇实验基地的紫心甘薯资源,对紫心甘薯中多糖的提取工艺进行了研究。对影响紫心甘薯多糖提取率的参数(料液比、提取温度、提取时间、提取次数、pH值、醇沉浓度)分别进行了单因素试验,在单因素试验的基础上,对其中3个主要影响因子(料液比、温度、时间) 进行正交试验,进一步比较了几种粗多糖脱蛋白和脱色的方法。最终确定了紫心甘薯多糖最佳提取条件为:料液比1:15、温度70℃、浸提2h、提取2次、pH中性、85%乙醇醇沉、3%三氯乙酸法脱蛋白、硅藻土脱色。 相似文献
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运用响应面分析法优化提取野西瓜种子蛋白质的最佳工艺条件,以蛋白质提取率作为响应值,对温度、pH值、提取时间和液料比进行中心组合设计。试验得出的二次模型的相关系数R2 = 96.7%。蛋白质的提取率主要受溶液的pH和液料比的影响。经过响应面分析得出野西瓜种子中蛋白质提取最佳条件为液料比23∶1(mL:g),提取时间50 min,pH值8.40和温度45 ℃,该条件下经模型预测得到的蛋白质提取率为17.63%,实际实验值为(17.19±0.09)%。 相似文献
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为研究酶法-超声波辅助提取红油椿老叶总黄酮工艺参数,以总黄酮得率为指标,经单因素试验筛选出影响总黄酮得率的4个因素:酶添加量、p H值、超声温度、料液比,并采用响应面试验优化提取工艺条件,并建立红油椿老叶中黄酮的高效液相色谱测定方法。结果表明,红油椿老叶总黄酮提取最佳工艺为纤维素酶添加量0.6%、p H 4.6、45℃酶解120 min,再经60%乙醇溶液、料液比1∶25(g/mL),超声功率140 W、超声温度62℃条件下提取40 min,连续提取2次,红油椿老叶总黄酮得率为14.98%。与酶法提取相比,酶法联合超声波法提取明显提高了总黄酮得率。高效液相色谱法测定结果表明,红油椿老叶总黄酮粗提物中含有芦丁、槲皮素和山柰酚,质量分数分别为0.16%、0.06%、0.03%。 相似文献