Two-stage biofilm-MBR for nitrogen removal and enhanced membrane performance

A two-stage biofilm-membrane bioreactor (MBR) was developed in this study. High total nitrogen removal (maximum: 81.4% with recycle ratio = 3.5) was observed by recycling the suspension liquid between an anaerobic FBBR and an aerobic MBBR. Very low (less than 60 mg/L) suspended solids was kept in the membrane unit, which could improve the membrane filtration performance. Membrane fouling was further reduced by increasing the recycle ratio. When influent organic load increased, the membrane fouling rate increased, coupling with higher FCOD and suspended solids (SS) values in the feed solution around the membrane.     

Nitrogen removal in an upflow sludge blanket (USB) reactor combined by aerobic biofiltration systems.

A new nitrogen removal process (up-flow sludge blanket and aerobic filter, USB-AF) was proposed and tested with real sewage. In the USB reactor, the larger part of influent organic and nitrogen matters were removed, and ammonia was effectively oxidized in the subsequent aerobic filter. The role of the aerobic filter was to convert ammonia into nitrate, an electron acceptor that could convert soluble organic matters into volatile suspended solid (VSS) in the USB. The accumulated as well as influent VSS in the USB was finally degraded to fermented products that were another good carbon source for denitrification. Total COD, settleable COD and soluble COD in the raw sewage were 325, 80 and 140 mg/l, respectively. Most unsettleable COD as well as some SCOD in the influent was successfully removed in the USB. TCOD removal in the anoxic filter was by denitrification with the recycled nitrate. Low COD input to the aerobic filter could increase nitrification efficiency, reduce the start-up period and save the aeration energy in the USB-AF system. About 95% of ammonia was nitrified in the aerobic filter with no relation to the influent ammonia concentration. Denitrification efficiency of the recycled nitrate in the anoxic filter was about 85, 83, and 72% at recycle ratios of 100, 200, and 300%, respectively. T-N removal efficiency was 70% at recycle ratio of 300%.     

Equalization characteristics of an upflow sludge blanket-aerated biofilter (USB-AF) system.

Equalization characteristics of the upflow sludge blanket-aerated bio-filter (USB-AF) were investigated with the fluctuated raw domestic sewage. Recycle of nitrified effluent from AF to USB triggered the equalization characteristics of the sludge blanket on both soluble and particulate organic matter. Increment of EPS in sludge blanket by nitrate recycle was detected and removal of turbidity and particulates increased at higher recycle ratios by bio-flocculation. Increased TCOD removal in the USB was due to both denitrification of recycled nitrate and entrapment of the particulate organic matter in sludge blanket. Capture of both soluble and particulate organic matter increased sludge blanket layer in the USB, which improved the reactor performances and reduced the organic load on the subsequent AF. Overall TCOD and SS removal efficiencies were about 98% and 96%, respectively in the USB-AF system. Turbidity in the USB effluent was about 44, 20 and 5.5 NTU, at recycle ratios of 0, 100 and 200%, respectively. Particle counts in the range 2-4 microm in the USB effluent were higher than those in influent without nitrate recycle, while particle counts in the range of 0.5-15 microm in the USB effluent decreased 70% at recycle ratio of 200%. The major constituent of EPS extracted from anaerobic sludge was protein and total EPS increased from 109.1 to 165.7 mg/g-VSS with nitrate recycle of 100%. Removal efficiency and concentration of T-N in the UBS-AF effluent was over 70% and below 16 mg/L, respectively.     

Effects of influent COD/N ratio and internal recycle ratio on nitrogen removal efficiency in the KNR process.

In this study, with the KNR process that has many advantages, the nitrogen removal efficiency of KNR was experimentally investigated at various COD/N ratios of influent conditions. The optimal operating condition of internal recycle ratio was evaluated. The TN removal efficiencies were 59.1, 72.5 and 75.9% at the COD/N ratios of 3, 5 and 7, respectively. These high removal efficiencies resulted from high denitrification rate in UMBR with high microorganism concentration. Furthermore, specific endogenous denitrification at MLVSS of 10.3 g/L that is similar to MLVSS in UMBR was over two times higher than that at MLVSS of 2.06 g/L. This result suggests that endogenous denitrification rate in UMBR is so high that the requirement of an external carbon source can be saved. As the internal recycle ratio increased from 100 to 400%, the TN removal efficiency also improved from 69.5 to 82.9%, and the optimal internal recycle ratio was 300%.     

Feasibility study on the removal of nitrous compounds with a hollow-fiber membrane biofilm reactor.

The objective of this study was to develop an integrated nitrogen treatment system using autotrophic organisms. A treatment system consists of an aerobic hollow-fiber membrane biofilm reactor (HfMBR) and anaerobic HfMBR. In the aerobic HfMBR, a mixture gas of air and O2 was supplied through the fibers for nitrification. Denitrification occurred in the anaerobic HfMBR using H2 as the electron donor. The treatment system was continuously operated for 190 days. NH4-N removal efficiencies ranging from 95% to 97% were achieved at NH4-N concentrations of influent ranging from 50 to 100 mg N/L. When glucose was added to the influent, the simultaneous nitrification and denitrification occurred in the aerobic HfMBR, and nitrogen removal rates were changed according to the COD/NH4-N ratio of influent. In the anaerobic HfMBR, autotrophic denitrification using H2 occurred and the removal rates achieved in this study were 23-58 mg N/m2 d. In this study, the achieved removal efficiency was lower than other study findings; however, the result suggested that this hybrid HfMBR system can be used effectively for nitrogen removal in oligotrophic water.     

倒置A2/O—MBR工艺处理低C/N生活污水脱氮性能研究

合肥塘西河再生水厂采用倒置A/O(缺氧/厌氧/好氧—预缺氧)—MBR工艺处理低C/N城市生活污水.研究了投加碳源前后各生化反应池中有机物及氮元素的变化规律,结果表明未投加外碳源时TN的去除率为52.3％,脱氮效果不理想.通过比较乙酸钠、甲醇和乙酸三种不同外加碳源的脱氮效果,可知乙酸钠反硝化速率最高,效果最好.当乙酸钠作外碳源,投加量为50 mgCOD/L时,TN的去除率明显提高,达到74.5％.此外,还分析了投加外碳源后整个工艺系统内COD、TN物质流动情况,结果表明,缺氧池和厌氧池是COD去除的主要单元体,好氧池对COD的去除贡献增强,体系中的TN去除总量在投加外碳源后有了明显的增加,较未投加外碳源时增加了26.5％.     

Anammox process for synthetic and practical wastewater treatment using a novel kind of biomass carriers

The anammox process, as an alternative to conventional nitrogen removal technologies, has abstracted much attention in recent years. In this study, one column-type reactor using a novel support material--net type acrylic fiber (Biofix)--was used for anammox treatment. The Biofix reactor was operated at 25 degrees C (peak summer temperature, 31.5 degrees C). Over 330 days of operation for synthetic wastewater treatment, the nitrogen loading rates of the reactor were increased to 3.6 kg-N/m3/d and T-N removal efficiencies reached to 81.3%. For the practical anaerobic sludge digester liquor treatment, the average TN removal efficiency of 72% was obtained. A protein substance was shown to be the most abundant extracellular polymeric substances (EPS) in the granular sludge with almost two times more in the attached sludge of the Biofix reactor. Considering the EPS levels and observation by scanning electron microscopy, the anammox granules in the Biofix reactor were showing dense state. Results of DNA analyses indicated that the KSU-1 strain might prefer relatively low nutrient levels, while the KU2 strain might be better suited for the high media concentration. Other kinds of bacteria were also identified with the potentials for consuming the dissolved oxygen in the influent and facilitating anammox bacteria surviving under aerobic conditions.     

林可霉素高浓度有机废水处理技术

采用厌氧颗粒和好氧活性污泥分别对内循环厌氧反应器(IC)和间歇式活性污泥法(SBR)进行污泥接种培养,研究水解酸化-IC-SBR工艺在林可霉素生产废水处理方面的运行效果。结果表明:在进水COD的质量浓度为6 000~9 000 mg/L,IC和SBR反应器中有机负荷分别为0.82 kg/(kg.d)和0.26 kg/(kg.d)左右的情况下,IC和SBR反应器分别运行60 d和7 d,COD平均去除率分别达到91%和61%,出水COD的质量浓度在300 mg/L以下,达到GB 8978—1996《污水综合排放标准》二级标准。     

Wastewater treatment with anaerobic membrane bioreactor and reverse osmosis.

Domestic wastewater from a new city district in Stockholm has been treated by an anaerobic membrane bioreactor (AMBR) followed by reverse osmosis (RO). The main objectives were to study the gas production, the reduction of organic matter and nutrient recovery. The AMBR was operated at 22 degrees C (equal to the average temperature in the influent) and a hydraulic retention time of 0.6 d. The results show that the reduction of organic matter, nitrogen and phosphorus over the AMBR was approximately 92, 9 and 9%, respectively. A stable gas production was registered throughout the evaluation period. The overall removal efficiency, i.e. including the RO, was >99% for TOC, >91% for Kj-N and about 99% for P. Adding a reverse osmosis (RO) unit to the AMBR makes it possible to produce a concentrated, nutrient rich product well suited for agricultural use. The quality of the concentrate is, in terms of nutrient concentration and heavy metal content, similar to source separated human urine, i.e. nitrogen content about 3 g N/L and <2 mg Cd/kg P. However, addition of acid is required to prevent precipitation/fouling of the RO. The total electricity use for operation for the system, including the RO-unit, is estimated to be 3-6 kWh/m3.     

Combined partial nitritation/Anammox system for treatment of digester supernatant.

One-year (2004) comprehensive investigations in a semi-industrial pilot plant (5 m3) were carried out with the aim of assessing the influence of operational parameters on the partial nitritation/Anammox system performance. In the system designed as a moving-bed biofilm reactor, the influent nitrogen load to the Anammox reactor was progressively increased and a stable Anammox bacterial culture was obtained. Interaction between subsequent aerobic and anaerobic conditions in the partial nitritation and Anammox reactors, respectively, granted conditions to remove nitrogen through the nitrite route. It implies that the oxygen supply can be limited to a high extent. A control strategy for the partial nitritation step relied on concomitant adjustment of the air supply with a variable influent nitrogen load, which can be monitored by both pH and conductivity measurements. In the Anammox reactor, an influent nitrite-to-ammonium ratio plays a vital role in obtaining efficient nitrogen removal. During the 1-year experimental period, the Anammox reactor was operated steadily and average nitrogen removal efficiency was 84% with 97% as the maximum value.     

低浓度基质下厌氧氨氧化反应器的启动及其运行性能

在低浓度基质条件下进行厌氧氨氧化反应器(ANAMMOX)的启动试验,先培养好氧硝化生物膜,然后转向厌氧环境培养厌氧氨氧化菌,经过294天成功启动反应器.在ANAMMOX稳定运行期,NH3-N的去除量:NO-2-N的去除量:NO-3-N的生成量为1:1.38:0.4.反应器的脱氮性能随着进水TN负荷的增加而提高,氮素去除负荷最高达到0.431 kg/(m3·d),并与进水TN负荷呈现良好的相关性.在上向流反应器中,NH3-N、NO-2-N浓度随水流沿滤柱高度不断降低,同时生成NO-3-N.沿水流方向至滤柱高120 cm处NH3-N和NO-2-N的去除率分别达到86.7%和近99%,TN的去除率达到85.4%.CODCr也随水流不断下降,pH则沿程上升.     

Influence of extracellular polymeric substances on nitrogen removal in an intermittently-aerated membrane bioreactor.

Influence of EPS on fouling of intermittent aeration MBR reactor (denitrification MBR) was investigated changing intermittent aeration cycle (10 minute-cycle and 120 minute-cycle) in laboratory-scale reactors using synthetic wastewater. EPS were extracted from bacterial cells using cation resin method and molecular weight fractioning of EPS was conducted using gel chromatography. In both of the reactors, nitrogen removal rate was almost 100% after 50th day although DO concentration was not very high during the aerated phase because of accumulation of nitrifying bacteria in the reactors. In the 120 minutes-cycle reactor, trans-membrane pressure increased more rapidly than in the 10 minutes-cycle reactor. The reason might be that EPS of more than 1000 kDa, which are the main fouling substances, are produced more rapidly in the 120 minute-cycle condition. It was also found that three peaks at around 100 kDa, 500 kDa and 2000 kDa are prominent in EPS in intermittent-aeration MBR irrespective of cycle and higher molecular weight EPS are decomposed to smaller molecular weight EPS on membrane surface.     

数学模型在污水处理厂优化控制过程中的应用

通过小试结合模拟计算,分析了某污水处理厂现有运行存在的不足.试验表明,内回流比对提高脱氮率的作用是有限制条件的;对于溶解氧的控制,要根据系统硝化反应的需要来确定;缺氧区/总体积的比例是影响脱氮效果的一个关键因素,在保证硝化不受影响的前提下,增大缺氧区使反硝化进行得更充分,有助于提高系统的脱氮能力.通过模拟计算,对其工艺参数进行了优化组合,在不改变池容、不增加其他处理设施的情况下提出了优化方案,确定其最佳工艺控制参数为:缺氧区/总体积的比例为0.45,混合液内回流比为200%,好氧区溶解氧2 mg/L.调整后的出水总氮为21.77 mg/L,去除率较改造前提高了4%,出水氨氮浓度为1.16 mg/L,去除率提高了7.2 %.     

Performance of ultrafiltration membrane process combined with coagulation/sedimentation.

Effects of coagulation/sedimentation as a pre-treatment on the dead-end ultrafiltration (UF) membrane process were studied in terms of membrane fouling and removal efficiency of natural dissolved organic matter, using Chitose River water. Two types of experiment were carried out. One was a bench scale membrane filtration with jar-test and the other was membrane filtration pilot plant combined with the Jet Mixed Separator (JMS) as a pre-coagulation/sedimentation unit. In the bench scale experiment, the effects of coagulant dosage, pH and membrane operating pressure on the membrane fouling and removal efficiency of natural dissolved organic matter were investigated. In the pilot plant experiment, we also investigated the effect of pre-coagulation/sedimentation on the membrane fouling and the removal efficiency of natural dissolved organic matter. Coagulation/sedimentation prior to membrane filtration process controlled the membrane fouling and increased the removal efficiency of natural dissolved organic matter.     

Effect of the pH influent conditions in fixed-film reactors for anaerobic thermophilic treatment of wine-distillery wastewater.

In anaerobic treatments, the pH conditions affect the efficacy and operation of the process. The main purpose of this research is to compare the effect of the pH influent on the performance of a high rate technology at laboratory scale, upflow anaerobic fixed-film reactor, treating distillery wastewater (wine vinasses) in thermophilic conditions. The results obtained shown that the pH influent influences the performance of the biodegradation process: the depurative efficiency is higher for the operation with alkaline influent. The operation with acid influent allows us to operate at organic loading rates (OLR) around 5.6 kgCOD/m3/d (hydraulic retention time: 1.5 days), maintaining total Chemical Oxygen Demand removals (CODr) of 77.2%; the operation with alkaline influent allows total CODr of 76.8% working at OLR around 10.5 kgCOD/m3/d. The greatest efficiency of substrate removal was 87.5% for OLR 3.2 kgCOD/m3/d and hydraulic retention time of 4.0 days operating with alkaline influent. Therefore, the operation with alkaline influent implicates senior levels of purifying efficiency for similar organic load rate.     

Performance evaluation of the BioCAST technology: a new multi-zone wastewater treatment system

A new wastewater treatment technology, called BioCAST, has been designed and developed for high rate and simultaneous removal of organic carbonaceous compounds as well as nitrogen and phosphorus, along with reduced sludge generation. The treatment system has two interlinked reactors containing four independent zones with different environmental conditions of aerobic, microaerophilic, anoxic and anaerobic for the biological treatment of wastewater, as well as two clarification zones and a filtration unit for solid-liquid separation. The treatment system contains suspended as well as fixed-film microorganisms. The performance evaluation of the BioCAST system was carried out at organic loading rates of 0.95 to 1.86 kg/m(3) d, and nitrogen and phosphorus loading rates of 0.02 to 0.08 kg/m(3) d and 0.014 to 0.02 kg/m(3) d, respectively. The results demonstrated high removal efficiencies of carbon and nitrogen throughout the operation period, reaching 98.9 and 98.3%, respectively. Phosphorus removal efficiency was lower than 50% during the first 160 days of operation but it increased with the increase of nitrogen loading rate above 0.05 kg/m(3) day and concomitant reduction of C/N ratio below 15. Phosphorus removal efficiency reached 94.1%, producing an effluent concentration of 1.4 mg/L after 225 days of operation. The overall biomass yield based on the consumed COD was 3.7%.     

水处理工艺过程中有机物分子量分布规律

针对太湖流域某湖泊水源有机物分子量分布的特性,考察饮用水处理各工艺单元对有机物的去除效应。结果表明:原水以小分子量有机物为主,小于1k Dalton的有机物占36.1%;常规工艺对大分子量有机物去除率高,尤其对分子量在10k Dalton以上的有机物去除率达32.7%以上,但对小分子量有机物去除率低,其中分子量小于1k Dalton的有机物反而增加了12.6%左右。超滤深度处理工艺中PES膜工艺对小分子量有机物,特别是分子量小于10kDalton的有机物去除率达76.4%,出水UV254值及SUVA值比砂滤出水分别下降了22.0%和11.2%;PVDF膜工艺对各分子量有机物均有较好的去除,但对大分子有机物去除效果优于小分子有机物,其出水UV254值及SUVA值比砂滤出水分别下降了28.8%和6.3%。     

Development of sequencing batch reactor with step feed and recycle.

SBR process shows high nitrogen and phosphorus removal in countries where separated sewers are used. On the other hand, removal efficiency is low in countries where combined sewers are used though the same SBR is applied. This is because the organic concentration (as BOD), which is used as carbon source for denitrification, of combined sewers is much lower than that of separated sewers. Almost all sewers in Korea are combined, and their BOD is low by about 1/2 over the level needed for denitrification. In this study, a SBR process that can optimise organic usage by step feed and recycle is thus developed to increase the removal efficiency of nitrogen and phosphorus, and the results show that the removal rates of BOD, T-N and T-P are 95.4, 81.4 and 86.1%, respectively, though influent BOD is low.     

塔式生物滤池处理城市污水的研究

尽管目前塔式生物滤池在城市污水处理中运用较少 ,但在有可资利用的地形时它可达到少动力或无动力运行 ,有较大的优势。研究了塔式生物滤池的工作参数及其处理特点。     

同步硝化反硝化脱氮研究

在控制SBR反应器保持良好的好氧状态条件下 ,考察进水COD/NH3比值对同步硝化反硝化脱氮效率的影响。同时也对同步硝化反硝化机理进行了初步的探讨。研究表明 ,进水COD/NH3比值越高 ,总氮去除率越高 ,同步硝化反硝化现象越明显。由该试验可以推断活性污泥菌胶团中异养硝化菌和好氧反硝化菌的存在。