膜分离技术分离纯化泰乐菌素的研究

简要介绍膜分离技术,采用超滤膜、纳滤膜对泰乐菌素发酵液进行脱色除蛋白试验。通过考查该体系膜通量、发酵液浓缩情况及滤液质量等,发现采用膜系统处理泰乐菌素发酵液在工艺、技术上是可行的,比传统过滤方式具备很多优越性。     

磁场强化膜分离土霉素发酵液工艺研究

引入磁场强化膜分离技术对板框过滤后的土霉素发酵液进行提纯和浓缩,研究结果表明,经0.4T磁段磁场强化后,PES20超滤膜及NF270纳滤分离性能均表现出较佳的正效应,土霉素产品质量较无磁场作用及原工艺有所提高。     

味精闭环生产新工艺

介绍通过膜分离技术开发出来的味精闭环生产工艺路线。该工艺采用无机陶瓷超滤膜将发酵液超滤;用浓硫酸水解截留液;过滤水解液,在滤液中得水解产物;混合渗透液与水解过滤液,进行谷氨酸结晶;最后用味精结晶母液纯化谷氨酸结晶。该工艺采用蒸发与冷却耦合连续结晶工艺,结晶收率高,且节能效果显著。     

动态微孔膜过滤特性研究

以旋叶膜滤机作为过滤设备对光合细菌发酵液为过滤物料进行了动态微孔膜过滤特性的研究。将膜技术与动态过滤技术相结合是膜分离技术的一个热点,集二者各自的优点,对其进行研究和开发,既有理论意义,又有实用价值。在实验中研究了操作条件如转速、压力、浓度对微孔膜过滤速率的影响。过滤速率的衰减基本可分为2个阶段,即速率下降阶段和稳态过滤阶段。     

动态膜滤速率特性的研究

以旋叶膜滤机作为过滤设备对光合细菌发酵液为过滤物料进行了动态微孔膜膜过滤特性的研究.将膜技术与动态过滤技术相结合是膜分离技术的一个热点,集两者各自的优点,对其进行研究和开发,既有理论意义,又有其实用价值.作者在实验中研究了操作条件如转速、压力、浓度对微孔膜过滤速率的影响.过滤速率的衰减基本可分为两个阶段,即速率下降阶段和稳态过滤阶段.     

一种有效延长膜使用寿命的中水处理工艺改进方法——郭玉同．CN 101274219

本发明涉及一种新的中水回用的改进工艺，针对利用膜分离技术处理的前期预处理过程中传统工艺的不足．采用二级磁分离技术和相应有磁过滤设备代替传统的过滤工艺和设备，通过添加磁种和絮凝剂，作为膜分离技术的前期预处理技术和设备。     

西梭霉素发酵液预处理工艺特性

应用正交试验研究了絮凝剂类型、质量浓度、发酵液pH值、动态过滤等因素对西梭霉素发酵液预处理效果的影响,获得适宜的预处理工艺条件。实验结果表明阴离子型聚丙烯酰胺A8025质量浓度为0.14g/L、pH=9—10时絮凝效果较好,经絮凝处理后的发酵液死端过滤常数比未经絮凝处理的发酵液死端过滤常数提高27.8倍,在此基础上若采用动态过滤不仅能进一步提高过滤速率,而且在过滤过程中西梭霉素的损失减少了7.9%。     

光催化膜分离工艺处理混凝废水的研究

采用光催化膜分离耦合工艺处理混凝废水。首先通过相转化法自制二氧化钛(TiO_2)改性聚偏氟乙烯(PVDF)光催化超滤膜;然后通过傅里叶红外光谱(FTIR)、扫描电子显微镜(SEM)、接触角测试仪、自制光催化膜分离工艺装置对膜结构、膜表面亲疏水性以及膜光催化和过滤性能进行测试分析;并通过改变进水流速、溶液pH,分析光催化膜分离工艺处理混凝废水的特点。研究结果表明,TiO_2成功嵌入到PVDF膜中,改性后膜接触角减小,具有一定的的光催化性能和更好的过滤性能。在混凝废水的处理过程中,进水流速主要影响膜通量,pH主要影响截留率,但光催化对膜分离过程的改进效果并不受工艺参数的影响。     

对1,3-丙二醇发酵液用金属纳滤膜过滤的研究

考察了l,3-丙二醇发酵液金属膜过滤在不同条件下的通量、收率及蛋白含量的变化。在此基础上,初步探讨了膜的过滤机理,确定了金属膜过滤的工艺参数。结果表明,100 nm膜适用于过滤1,3-丙二醇发酵液,按料液体积20%从开始过滤2h后就持续加入去离子水,在4.2/3.6 MPa压力下,60℃,pH值为7的条件下,5h内完成过滤,并且收率达到87%以上,运行时间短而且对后续工艺压力最小。     

膜分离技术在发酵液提取浓缩中的应用

发酵液组分复杂,分离技术繁多,同其它方法相比膜分离技术以其独特的优势在该领域中获得推广。本文简单概述了膜分离技术在多糖、抗生素、饮料、酶制剂及乙醇发酵液提取浓缩中的应用以及国内外在这一领域的进展。对膜分离过程应用于该领域的前景进行了分析。     

头孢菌素C的分离研究进展

通过分析头孢菌素C发酵液的特点,论述传统分离过程,同时介绍了新技术膜过滤的应用情况并与传统的工艺进行比较,指出未来膜技术在分离头孢菌素C中有广阔的应用前景.     

膜分离技术应用于草浆造纸清洁生产

在错流流速5 m•s^-1、压力0.3 MPa 、温度20℃条件下,用0.2、0.5、0.8 μm无机陶瓷膜对草浆造纸黑液进行了膜滤研究.考察了无机陶瓷膜对污染物的截留效果,探索膜的清洗与膜分离技术用于草浆造纸废液循环回用的途径.     

生物乙醇原位分离技术的研究进展

生物乙醇是一种重要的可再生生物燃料,使用生物乙醇可大幅减少温室气体排放。为了建立更高效低能耗的生物乙醇回收工艺,原位分离（ISPR）技术应运而生。本文综述了近年来乙醇原位分离的研究进展,从原理及应用等进行多方面详细地介绍,包括气提、真空发酵、吸附、液-液萃取、渗透汽化、膜蒸馏等分离技术。针对分离性能、能耗成本等问题分析了不同分离技术耦合发酵过程的优势及不足,重点回顾了以渗透汽化为代表的膜分离技术,总结了渗透汽化膜材料的选择以及膜的制备方法,旨在提升乙醇分离膜性能优化乙醇分离工艺。为整合不同分离技术的特点及优势,聚焦多级耦合分离系统的开发对各级分离技术联用的性能及潜力进行剖析与评价,并在此基础上研判其发展前景。     

Processing of ethanol fermentation broths by Candida krusei to separate bioethanol by pervaporation using silicone rubber‐coated silicalite membranes

BACKGROUND: Pervaporation employing ethanol‐permselective silicalite membranes as an alternative to distillation is a promising approach for refining low‐concentration bioethanol solutions. However, to make the separation process practicable, it is extremely important to avoid the problems caused by the adsorption of succinate on the membrane during the separation process. In this work, the pervaporation of an ethanol fermentation broth without succinate was investigated, as well as the influence of several fermentation broth nutrient components. RESULTS: Candida krusei IA‐1 produces an extremely low level of succinate. The decrease in permeate ethanol concentration through a silicone rubber‐coated silicalite membrane during the separation of low‐succinate C. krusei IA‐1 fermentation broth was significantly improved when compared with that obtained using Saccharomyces cerevisiae broth. By treating the fermentation broth with activated carbon, bioethanol was concentrated as efficiently as with binary mixtures of ethanol/water. The total flux was improved upto 56% of that obtained from the separation of binary mixtures, compared with 43% before the addition of activated carbon. Nutrients such as peptone, yeast extract and corn steep liquor had a negative effect on pervaporation, but this response was distinct from that caused by succinate. CONCLUSION: For consistent separation of bioethanol from C. krusei IA‐1 fermentation broth by pervaporation, it is useful to treat the low nutrient broth with activated carbon. To further improve pervaporation performance, it will be necessary to suppress the accumulation of glycerol. Copyright © 2009 Society of Chemical Industry     

发酵液中乳酸的分离提取研究进展

乳酸是合成聚乳酸的原料,生物法制备乳酸是目前工业上生产乳酸的主要方法。但乳酸发酵液成分复杂,后续的分离提纯过程成了制约乳酸生产的技术瓶颈和难点,也决定着乳酸的品质与收率。本文对乳酸发酵液的主要的分离提取工艺进行了介绍,包括结晶分离技术、酯化水解法、萃取法、分子蒸馏法、膜分离法、吸附法及与发酵耦合的原位分离技术。并提出单一的分离技术很难有效提取乳酸,需将多种技术集成、改良提纯工艺路线。其中,将各种新型高效的集成技术与发酵过程的有机结合,实现连续或半连续的发酵过程,可提高乳酸产率和产品质量,有望形成高效率、高品质、低污染、低能耗、可工业化的乳酸提纯工艺路线。     

Pervaporative separation of bioethanol produced from the fermentation of waste newspaper

In this study, a hydrophobic polymeric polydimethylsiloxane (PDMS) membrane was used for the pervaporative separation of bioethanol produced from fermentation of lignocellulosic biomass (waste newspaper) and glucose. As a preliminary study, the pervaporation permeation performance showed strong dependence on feed concentration and temperature. The pervaporation of bioethanol produced by the fermentation of waste newspaper by Saccharomyces cerevisiae decreased process performance. However, the process performance was restored reversibly by water cleaning. The pervaporative separation of bioethanol from the fermentation of waste newspaper was carried out without any significant decreasing process performance in the study.     

乙醇发酵原位分离产物耦合方法研究进展

乙醇发酵过程存在明显的产物抑制现象,严重制约了乙醇产率的提高。乙醇发酵与产物分离的耦合可有效解决这一难题,目前乙醇发酵耦合产物分离的方法主要有乙醇气提发酵、乙醇真空发酵、乙醇吸附发酵、乙醇萃取发酵、乙醇膜分离发酵等。作者主要概述了各种乙醇发酵耦合产物分离方法的研究进展,并对今后乙醇发酵耦合产物分离研究开发提出了展望。     

Stabilized production of highly concentrated bioethanol from fermentation broths by Zymomonas mobilis by pervaporation using silicone rubber‐coated silicalite membranes

Since pervaporation performance of ethanol‐permselective silicalite membrane, which is an aluminum‐free hydrophobic zeolite, in the separation of fermentation broths by yeast are negatively affected by succinic acid, the potential of pervaporation using silicone rubber‐coated silicalite membranes of ethanol fermentation broths, not containing succinic acid, by Zymomonas mobilis was investigated for the reliable production of concentrated bioethanol. In the separation of fermentation broths, the pervaporation performance was influenced by nutrients used for the preparation of fermentation broths. In the separation of a broth prepared with yeast extract, pervaporation performance was greatly compromised by accumulation of a substance(s) having an ultraviolet absorption maximum at approximately 260 nm not only in total flux, but also in permeate ethanol concentration compared to the separation of binary ethanol/water mixtures. When supplying a prepared broth with corn steep liquor without the accumulation of a substance(s) having an ultraviolet absorption maximum at approximately 260 nm, the permeate ethanol concentration did not decrease. Treating the prepared broth with activated carbon was effective in restraining the decrease in total flux. Pervaporation performance is also deteriorated by the adsorption of lactic acid contained in corn steep liquor onto the silicalite crystals. In the separation of ternary mixtures of ethanol/water/lactic acid, accomplished by adjusting the ternary mixtures to pH > 5, more than 90% of the permeation flux in the separation of binary ethanol/water mixtures was obtained, and the permeate ethanol concentration was comparable to that obtained in the separation of binary mixtures. For stably performing pervaporation, it is important to prepare ethanol fermentation broths by Zymomonas mobilis in which lactic acid concentration is as low as possible. Copyright © 2007 Society of Chemical Industry     

头孢菌素C发酵液膜过滤的评价指标及其控制

发酵液的过滤澄清是头孢菌素C（CPC）分离与提纯的首要环节,对CPC产品的质量、成本和生产进度等有着很大的影响。本文针对CPC发酵液的膜过滤过程,分别给出了过滤过程评价指标和滤液质量评价指标。通过对部分评价指标的分析,讨论了影响各指标水平的具体因素,并建立起了有关参量间的量化模型,从而为滤液质量的控制与生产过程的优化提供了途径和方法。