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通过在培养基中添加Cr3 制备富铬酵母,测定富铬酵母生物量及铬的含量进行培养条件优化实验,结果表明,培养温度28℃,摇床转数220r/min,培养基CrCl3·6H2O添加量为20mg/L,装液量50mL/250mL三角瓶,接种量5%(v/v),pH值为6.0,培养时间72h,获得的富铬酵母生物量可达(12.11±0.14)g/L,铬含量可达(184.68±0.07)μg/g,铬总含量达到了(2236.48±26.62)g/L。其中有机铬含量为88.9%。对空白酵母和富铬酵母进行红外光谱分析,比较了酵母富集Cr3 前后吸收峰的变化,并对空白酵母和富铬酵母中的17种氨基酸的组成进行了分析。 相似文献
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啤酒酵母富铬条件的初步研究 总被引:5,自引:1,他引:5
本文研究了培养温度、摇瓶时间、接种量,培养基中添加铬盐和营养成分对啤酒酵母富铬作用的影响;比较啤酒酵母、异常汉逊氏酵母、假丝酵母、热带假丝酵母和粉状毕赤酵母的富铬能力。取得啤酒酵母富铬作用的优化条件:麦芽汁培养基中,添加2.0μg/mLCrCL_3·6H_2O、2.0μg/mL的尿素,每瓶(500 mL)接种2 ml啤酒酵母菌母液(3.2×10~6酵母细胞/mL)、30℃、140往返/min摇瓶48 h,可制得总铬含量和有机铬含量较高的铬酵母。 相似文献
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本研究明确了制备富硒、铬酵母的最佳工艺条件,即培养基中硒离子浓度为 20μg/ml、铬离子浓度为40μg/ml、培养时间为48h、接种量为15%.在最优工艺条件下所制备的富硒、铬酵母中硒、铬含量分别为 499.02、257.63μg/g,吸收率分别为55.53%和14.33%.有机硒和有机铬的转化率分别为97.72%和90.09%,均达到较高水平. 相似文献
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建立了利用啤酒废酵母制备富铬酵母的新工艺。通过正交试验分析确定了最佳工艺条件为在麦芽汁中添加啤酒废酵母,使料液比达到1:1,在麦芽汁中分3次添加CrCl3搭液,使其Cr^3+达到200μg/mL,摇瓶培养16h,制得的产品中有机铬浓度可达195.6μg/g。 相似文献
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建立了利用啤酒废酵母制备富铬酵母的新工艺。通过正交试验分析确定了最佳工艺条件为在麦芽汁中添加啤酒废酵母,使料液比达到1:1,在麦芽汁中分3次添加CrCl3搭液,使其Cr^3+达到200μg/mL,摇瓶培养16h,制得的产品中有机铬浓度可达195.6μg/g。 相似文献
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木醋杆菌发酵培养基优化及发酵方式的探讨 总被引:3,自引:0,他引:3
以木醋杆菌(Acetobacter xylinum)为出发菌株,细菌纤维素产量为指标,通过正交试验优化发酵培养基,其最优组成为葡萄糖15g/L、蛋白胨10g/L、酵母膏5g/L、玉米浆40mL/L、磷酸三钠3g/L、柠檬酸钠1g/L、硫酸镁2g/L、氯化钙0.2g/L、硫酸亚铁0.03g/L、乙醇10mL/L。适宜的发酵方式为先130r/min振荡培养10h,后静置培养10d。经验证试验,细菌纤维素产量可达3.81g/L。 相似文献
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蛋白质水解度测定方法综述 总被引:27,自引:0,他引:27
对目前国内外常用的蛋白质水解度测定方法进行了综述,其中pH—state方法是通过滴定水解过程中释放的质子测定DH;OPA、TNBS及国内常用的水合茚三酮和甲醛等测定方法是利用游离氨基的反应测定DH。 相似文献
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研究了聚多元羧酸盐的合成方法及反应机理,将其应用于洗涤剂和PVC制品中分别代替三聚磷酸钠和邻苯二甲酸二丁酯,证明有良好效果。 相似文献
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SUMMARY: A partially purified immunoglobulin G (lgG) solution prepared from the serum of species to be tested was heated to the specifications for sausages. The resulting supernatant fluid was decanted and the precipitate washed with saline and used to immunize rabbits. The supernatant fluid was used to sensitize tanned sheep red blood cells. The immune serum was rendered monospecific by absorptions with heterologous, heated lgG precipitates. A sample of monospecific immune serum was absorbed with a washed homogenate of sausage. Aliquots of the monospecific immune serum, both untreated and sausage absorbed, were tested with cells sensitized with the homologous heated lgG supematant fluid. A significant reduction of titer by sausage absorption indicated that the sausages contained the meat homologous to the immune serum. 相似文献
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SUMMARY— The mechanism and generality of the known stabilization against autoxidation conferred on linoleic acid by certain basic amino acids, such as lysine and arginine, was investigated. Basic amino acids were the only class of compounds found to confer the effect. However, the smallest basic amino acid, 2,3-diaminopropionic acid, was not effective, nor was αβω-diaminc acid, 3,6-diaminohexanoic acid, although a simple isomer of lysine. The stabilization was observed only in the solid phase. Inclusion of sodium chloride in the solid matrix was deleterious to the effect. A large number of physical and chemical observations were made and correlated but it has not been possible to draw detailed conclusions about the mechanism of stabilization, nor can a detailed structure of the stabilized complex be suggested. The cause of the phenomenon appears to be closely associated with the physical arrangement of the ions in the crystal lattice. 相似文献
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《中国服装(北京)》2013,(19):130-133
一场流光溢彩、赏心悦目的展览,一段百年风尚演进的传奇旅程,一次东西方文化艺术的完美对话。2013年9月13日,“博萃臻艺一中西方珍宝艺术展”在辽宁省博物馆举行了隆重的开幕仪式,法兰西共和国驻华大使白林女士、辽宁省文物局局长丁辉先生、辽宁省博物馆馆长马宝杰先生、卡地亚全球总裁兼首席执行官邓阁仕先生、卡地亚区域行政总裁(北亚洲)陆慧全先生、卡地亚中国区首席执行官陆意斯先生、辽宁省文物店总经理张春鹰先生,以及众多文化界与文博界的贵宾齐聚一堂,共同见证了这场文化艺术盛事。 相似文献
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Hop oil emulsions prepared from different varieties of hops have been found to exhibit enhanced physical stability on the addition of blends of the emulsifiers Span 20/Tween 80 or Span 60/Tween 60. Examination of the particle size and volume distributions of an emulsion by use of a Coulter Counter was found to be an excellent method of monitoring its stability. An indication as to the relative efficiency of emulsifiers can be obtained from Coulter Counter measurements on hop oil emulsions after storage for 4 days. The use of an ultracentrifuge provies a rapid means of testing emulsion stability and hence the effectiveness of emulsion stabilizers. 相似文献
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矩阵乘积的行式,列式 总被引:1,自引:0,他引:1
张静 《郑州轻工业学院学报(自然科学版)》1997,12(3):72-74
给出了m×m矩阵与m×n矩阵的行(列)式的表达式.若A=a11a12…a1ma21a22…a2m……am1am2…ammB=b11b12…b1nb21b22…b2n……bm1bm2…bmn分别是m×m,m×n矩阵,则|A||B|=|AB|+∑i1<i2<…<itj1<j2<…<jt1≤t≤mn-t≥mNBi1i2…itj1j2…jtNAB1…m(-1)st+1jt+1…(-1)snjn其中i1,i2,…,it是1,2,…,m中t个数码;j1,j2,…,jt,jt+1,…,jn是1,2,…,n的一个排列;sr=π(j1,j2,…,jt,jr)(r=1,2,…,n)是排列j1,j2,…,jt,jr的反序数. 相似文献
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M. L. Brown 《Journal of the Institute of Brewing》1970,76(1):61-65
The lengths of chains of cells of Saccharomyces cerevisiae were studied during fermentation. Pitching yeast generally contained about half of the total number of cells as two-celled chains. The chain lengths varied during the subsequent fermentation and the variations were characteristic of the strain. Electronic counting assessments of chain length were unreliable. 相似文献
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张仲民 《郑州轻工业学院学报(自然科学版)》1993,8(3):21-24
本文基于点的复合运动理论,用球坐标法推导出点的速度及加速度的计算公式,进一步论证了理论力学中点的速度,加速度公式的正确性。 相似文献
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