Applications of textured surfaces on bubble trapping and degassing for microfluidic devices

This paper introduces a passive degassing mechanism using textured surfaces to trap and transport bubbles, and then using hydrophobic porous membranes to vent out bubbles in a microfluidic system. The bubble trapping ability is achieved by creating nanostructures to promote bubble nucleation and coalescence on the sidewalls of KOH-etched concave pits in a silicon substrate. The substrate, which is bonded with a porous membrane, is placed in a liquid system with chemically generated CO₂ bubbles to examine the degassing ability. The results validate that the bubbles can be easily trapped on the surfaces with nanostructures, and then vented through the porous membrane. Our proposed approach possesses the advantage of simple fabrication, great structure robustness, and effective bubble trapping and removing abilities, which show their great potential as economic, passive means of preventing the gas byproducts from blocking surfaces and improving the efficiency of microfluidic systems during operations.     

A novel three-dimensional microfluidic platform for on chip multicellular tumor spheroid formation and culture

The formation of three-dimensional (3D) multicellular cell spheroids such as microspheres and embryoid bodies has recently gained much attention as a useful cell culture technique, but few studies have investigated the suitability of glass for spheroids formation and culture. In this work, we present a novel three-dimensional microfluidic device made of poly(dimethylsiloxane) (PDMS) and glass for the easy and rapid synthesis and culture of tumor spheroid. The cell culture unit is composed of an array of microwells on the bottom of a glass plate, bigger microwells and elastomeric microchannels on the top of a PDMS plate. Cell suspension can be easily introduced into the cell culture unit and exchange with the external liquid environment by the microfluidic channels. A single tumor spheroid can be formed and cultured in each glass cell culture chamber, the surface of which was modified with poly(vinyl alcohol) to render it to be resistant to cell adhesion. As the cell culture medium could be replaced, spheroids of the human breast cancer (MCF-7) cells were cultured on the chip for 3 days, reaching the diameters of about 150 μm. Furthermore, the MCF-7 cells were successfully cultured on the chip in 2D and 3D culture modes. Results have shown that glass is well suitable for multicellular tumor spheroids culture. The established platform provides a convenient and rapid method for tumor spheroid culture, which is also adaptable for anticancer drug screening and fundamental biomedical research in cell biology.     

Research on the comprehensive traffic state evaluation model linked with drivers’ perception under the vehicle networking

Unified judgment standards and methods are often adopted to identify traffic state in certain road network based on traffic flow parameters. However, drivers often have different perceptions about the traffic state on different road sections, since their expectations on traffic state vary more or less from each other on different road sections. In particular, under the vehicle networking, out of considerations for safety and other relevant factors, requirements for the correlation and coordination of running vehicles have also raised significantly. Therefore, it is necessary to take driver’s perception about the driving conditions of certain road sections into consideration to adjust the release of traffic state. This paper has provided a comprehensive traffic state evaluation model linked with driver’s perception under the vehicle networking. The authors first establish an ANFIS model based on the T–S model and then conduct statistical analysis on drivers’ perceptions about certain traffic state. At last, the authors use the results of statistical analysis as regulatory factors to amend the parameters input through ANFIS. Through simulation, this paper has demonstrated that the model established has a high rate of convergence, a high identification precision and the generalization ability to conduct researches on the identification of traffic state.     

基于多特征融合和SVM分类器的植物病虫害检测方法

针对农业领域植物病虫害检测问题,提出一种基于高清视频图像融合特征的支持向量机(SVM)的检测方法,实现农业生产中植物病虫害的快速检测。对每幅植物叶片图像的颜色、HSV、纹理和方向梯度直方图四种特征采用基于特征包的多特征融合方法,形成特征向量,并利用SVM分类器进行训练分类。对单特征与融合特征的SVM分类器性能进行试验比较,所提出的方法具有较高的准确率。     

3,4,5-三甲基叔丁基苯的合成与分析

介绍了以连三甲苯和氯代叔丁烷为起始原料 ,在自制的非均相催化剂W 1 0 0作用下一步合成3,4 ,5 -三甲基叔丁基苯的简便方法。经探索性试验、正交试验和单因素试验确定了最佳反应条件 :连三甲苯与氯代叔丁烷物质的量比为n连三甲苯∶n氯代叔丁烷 =1∶1 ,催化剂W 1 0 0的用量占连三甲苯的质量分数为 2 % ,反应温度 5℃ ,反应时间 7～ 8h ,在此条件下 ,3,4 ,5 -三甲基叔丁基苯的收率达到 79%以上     

循环冷却水中磷酸盐及其总磷的快速测定

本文用磷钼蓝比色法测定工业循环冷却水中的磷含量，并对测定条件进行了优选。该方法操作简便，准确可靠，适用于一般的磷系水处理循环水中磷的测定。     

位置大数据隐私保护研究综述

大数据时代移动通信和传感设备等位置感知技术的发展形成了位置大数据,为人们的生活、商业运作方法以及科学研究带来了巨大收益.由于位置大数据用途多样,内容交叉冗余,经典的基于“知情与同意”以及匿名的隐私保护方法不能全面地保护用户隐私.位置大数据的隐私保护技术度量用户的位置隐私,在信息论意义上保护用户的敏感信息.介绍了位置大数据的概念以及位置大数据的隐私威胁,总结了针对位置大数据隐私的统一的基于度量的攻击模型,对目前位置大数据隐私保护领域已有的研究成果进行了归纳.根据位置隐私的保护程度,可以把现有方法总结为基于启发式隐私度量、概率推测和隐私信息检索的位置大数据隐私保护技术.对各类位置隐私保护技术的基本原理、特点进行了阐述,并重点介绍了当前该领域的前沿问题：基于隐私信息检索的位置隐私保护技术.在对已有技术深入分析对比的基础上,指出了未来在位置大数据与非位置大数据相结合、用户背景知识不确定等情况下保护用户位置隐私的发展方向.     

阳极炭块裂纹问题的探讨

分析阳极裂纹的形态外貌和引起阳极裂纹的应力原因归类，指出除固体材料应力变化、内部缺陷外，生块与焙烧裂纹多与半塑性态下物相转变引起的应力有关。研究并指出了一系列工艺因素对阳极裂纹的影响。     

上海化学工业区的建设必须依托深水海港的开发

分析上海港目前特定的环境和制约因素，指出上海化学工业区的建设必须依托深水海港的开发，提出舟山群岛中的大衢山是建设深水港岛的理想基地，并作了与上海陆地联系的设想。     

碱—碳酸盐反应和pH值

热力学计算表明,碱—碳酸盐反应的AG_(298)~0=-12.19kJ。这是去白云石化反应得以进行的化学推动力。文中列出了计算所得的白云石稳定区和不稳定区的范围。由此得出,溶液中的pH愈高,CO_3~(2-)浓度愈低,则白云石愈不稳定。这就从理论上阐明了为什么水泥中碱含量愈高,碱—碳酸盐反应愈烈,膨胀破坏作用就愈大。混合材掺量很高时,才能显著降低水泥石液相中的pH值,从而缓和碱—碳酸盐反应。这再一次证明碱—碳酸盐反应与去白云石化密切相关。实验和理论证明,碱—碳酸盐反应是由于去白云石化在原地产生,这种局部化学反应和结晶压是引起膨胀的根本原因。要抑制碱—碳酸盐反应,防止混凝土工程遭受破坏,最根本的途径是采用高混合材掺量的低碱水泥。