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31.
32.
Shuhong Mao Boyuan Hua Na Wang Xiaojie HU Zhijiang Ge Yanqing Li Sha Liu Fuping Lu 《Journal of chemical technology and biotechnology (Oxford, Oxfordshire : 1986)》2013,88(2):287-292
BACKGROUND: The improved efficiency of steroid biotransformation using the biphasic system is generally attributed to the positive effect on the solubility of substrate in aqueous media. A promising alternative for the application of organic solvents in biphasic systems is the use of ionic liquids (ILs). This study aims to investigate the applicability of the biphasic ILs/water system for 11α hydroxylation of 16α, 17‐epoxyprogesterone (HEP) by Aspergillus ochraceus. RESULTS: Of the seven ILs tested, [C3mim][PF6] exhibited the best biocompatibility, with markedly improved biotransformation efficiency. In the [C3mim][PF6]‐based biphasic system, substrate conversion reached 90% under the condition in which buffer pH, volume ratio of buffer to ILs, cell concentration, and substrate concentration were 4.8, 10/1, 165 g L?1 and 20 g L?1, respectively. This is more efficient than that of the monophasic aqueous system. The effects of the cations and anions of these ILs on the 11α hydroxylation of 16α, 17‐epoxyprogesterone (HEP) by A. ochraceus is also discussed. CONCLUSION: The above results showed that IL/water biphasic system improved the efficiency of 11α hydroxylation of 16α, 17‐epoxyprogesterone (HEP) by A. ochraceus, thus suggesting the potential industrial application of ILs‐based biphasic systems for steroid biotransformation. © 2012 Society of Chemical Industry 相似文献
33.
紫外光对蛋白酶米曲霉T_(213)菌株经5次照射,获得米曲霉T_(213)菌株,其麸曲蛋白酶活力较出发菌株增加近1倍,达6840U/g。再用25mwHe—Ne激光对T_(213)菌株进行诱变处理4次,筛选出T_(222)变异株,其麸曲蛋白酶活力较T_(213)菌株增加31%。达8960U/g。 相似文献
34.
黄干粉是玉米淀粉厂玉米提取淀粉后的下脚料,含粗蛋白30%左右.利用黄干粉作原料,在PH6.0条件下用沪酿3.042米曲霉制曲,发酵生产酱油获得成功,对产品进行的理化指标检验结果表明,酱油达到部颁三级标准. 相似文献
35.
1 Introduction Heavy metals like copper, mercury, chromium, cadmium, lead, nickel and zinc cause serious threat to environment, animals and human for their extreme toxicity[1]. Many industries including metal plating, mining, battery, pigment, dyestuff a… 相似文献
36.
The biosolids accumulation and biodegradation of domestic wastewater treatment plant (DWTP) sludge by filamentous fungi have been investigated in a batch fermenter. The filamentous fungi Aspergillus niger and Penicillium corylophilum isolated from wastewater and DWTP sludge was used to evaluate the treatment performance. The optimized mixed inoculum (A. niger and P. corylophilum) and developed process conditions (co-substrate and its concentration, temperature, initial pH, inoculum size, and aeration and agitation rate) were incorporated to accelerate the DWTP sludge treatment process. The results showed that microbial treatment of higher strength of DWTP sludge (4% w/w of TSS) was highly influenced by the liquid state bioconversion (LSB) process. In developed bioconversion processes, 93.8 g/kg of biosolids was enriched with fungal biomass protein of 30 g/kg. Enrichment of nutrients such as nitrogen (N), phosphorous (P), potassium (K) in biosolids was recorded in 6.2% (w/w), 3.1% (w/w) and 0.15% (w/w) from its initial values of 4.8% (w/w), 2.0% (w/w) and 0.08% (w/w) respectively after 10 days of fungal treatment. The biodegradation results revealed that 98.8% of TSS, 98.2% of TDS, 97.3% of turbidity, 80.2% of soluble protein, 98.8% of reducing sugar and 92.7% of COD in treated DWTP sludge supernatant were removed after 8 days of microbial treatment. The specific resistance to filtration (SRF) in treated sludge (1.4x10(12) m/kg) was decreased tremendously by the microbial treatment of DWTP sludge after 6 days of fermentation compared to untreated sample (85x10(12) m/kg). 相似文献
37.
As a new mutagenetic method, low-energy ion implantation has been used widely in many research areas in recent years. In order to obtain some industrial strains with high xylanase yield, the wild type strain Aspergillus niger A3 was mutated by means of nitrogen ions implantation (10 keV, 2.6× 10^14 ~ 1.56 × 10^15 ions/cm^2) and a mutant N212 was isolated subsequently. However, it was found that the initial screening means of the high-yielding xylanase strains such as transparent halos was unfit for first screening. Compared with that of the wild type strain, xylanase production of the mutant N212 was increased from 320 IU/ml to 610 IU/ml, and the optimum fermentation temperature was increased from 28 ℃ to 30 ℃. 相似文献
38.
N. Vigneshwaran N.M. Ashtaputre P.V. Varadarajan R.P. Nachane K.M. Paralikar R.H. Balasubramanya 《Materials Letters》2007,61(6):1413-1418
The fungus, Aspergillus flavus when challenged with silver nitrate solution accumulated silver nanoparticles on the surface of its cell wall in 72 h. These nanoparticles dislodged by ultrasonication showed an absorption peak at 420 nm in UV-visible spectrum corresponding to the plasmon resonance of silver nanoparticles. The transmission electron micrographs of dislodged nanoparticles in aqueous solution showed the production of reasonably monodisperse silver nanoparticles (average particle size: 8.92 ± 1.61 nm) by the fungus. X-ray diffraction spectrum of the nanoparticles confirmed the formation of metallic silver. The Fourier transform infrared spectroscopy confirmed the presence of protein as the stabilizing agent surrounding the silver nanoparticles. These protein-stabilized silver nanoparticles produced a characteristic emission peak at 553 nm when excited at 420 nm in photoluminescence spectrum. The use of fungus for silver nanoparticles synthesis offers the benefits of eco-friendliness and amenability for large-scale production. 相似文献
39.
海洋来源真菌烟曲霉(Aspergillus fumigatus)次级代谢产物研究(Ⅰ):生物碱 总被引:1,自引:0,他引:1
对一株海洋来源的微生物中的次级代谢产物进行了研究,从中分离并鉴别了5个喹唑啉生物碱类化合物(1~5),分别为fumiquinazoline G(1),fumiquinazoline F(2),fumiquinazoline A(3),fumiquinazoline C(4)和fumiquinazoline D(5)。 相似文献
40.
Qing-Xian Bai Yi Huan Jian-Hong Wang Li-Jie Yang Hong-Juan Dong 《International journal of molecular sciences》2012,13(9):11063-11070
Aspergillosis remains to be a life-threatening complication in immunocompromised patients. However, Aspergillus infection can be observed in non-immunocompromised individuals in rare cases. We report a case of liver aspergilloma in a chronic aplastic anemia patient under relatively intact immune status. Therapeutic strategy for this rare condition was extensively discussed and caspofungin acetate single agent first-line therapy was applied after careful consideration. Encouraging clinical and radiologic improvements were achieved in response to the antifungal salvage. Our long-term follow-up study also revealed a favorable prognosis. Based on this experience, we suggest caspofungin acetate as first-line therapy for treatment plans of liver aspergilloma. 相似文献