Role of the HSP70 Co-Chaperone SIL1 in Health and Disease

Cell surface and secreted proteins provide essential functions for multicellular life. They enter the endoplasmic reticulum (ER) lumen co-translationally, where they mature and fold into their complex three-dimensional structures. The ER is populated with a host of molecular chaperones, associated co-factors, and enzymes that assist and stabilize folded states. Together, they ensure that nascent proteins mature properly or, if this process fails, target them for degradation. BiP, the ER HSP70 chaperone, interacts with unfolded client proteins in a nucleotide-dependent manner, which is tightly regulated by eight DnaJ-type proteins and two nucleotide exchange factors (NEFs), SIL1 and GRP170. Loss of SIL1′s function is the leading cause of Marinesco-Sjögren syndrome (MSS), an autosomal recessive, multisystem disorder. The development of animal models has provided insights into SIL1′s functions and MSS-associated pathologies. This review provides an in-depth update on the current understanding of the molecular mechanisms underlying SIL1′s NEF activity and its role in maintaining ER homeostasis and normal physiology. A precise understanding of the underlying molecular mechanisms associated with the loss of SIL1 may allow for the development of new pharmacological approaches to treat MSS.     

Detection of 5-HMF in apple juice with artificial sensing systems

5-HMF (5-hydroxymethyl-furfural) is a product of thermal treatment and is increasingly considered a food contaminant. Here, different concentrations of 5-HMF were measured in apple juice to evaluate the performance of the electronic nose (EN) and electronic tongue (ET) as rapid detection techniques for 5-HMF when coupled with chemometric analysis. Principal component analysis (PCA) and linear discriminant analysis (LDA) evaluated the discrimination capacity of EN and ET for 5-HMF. Loading analysis examined the discrimination contribution of the EN sensors. Partial least square (PLS) regression analysis established a quantitative prediction model for different concentrations of 5-HMF based on EN and ET data set. The optimal models had a coefficient of determination (R²) of 0.926 and a root-mean-square error of prediction (RMSEP) of 0.4168 in EN; there were R² of 0.914 and RMSEP of 0.5836 in ET. These results demonstrate that EN and ET coupled with chemometric analysis are two promising approaches for the rapid and online detection of 5-HMF in apple juice.     

Red-shift and improved afterglow of Sr3Al2-xBxO5Cl2:Eu2+, Dy3+ phosphors via a cation substitution strategy

Sr₃Al_2-xB_xO₅Cl₂:Eu²⁺, Dy³⁺ (x = 0, 0.2, 0.4) long persistent phosphors were prepared via solid-state process. The pristine Sr₃Al₂O₅Cl₂:Eu²⁺, Dy³⁺ phosphor exhibits orange/red broad band emission around 609 nm, which can be attributed to the electric radiation transitions 4f⁶5 d¹→4f⁷ of Eu²⁺. Upon the same excitation, the B³⁺-doped Sr₃Al_2-xB_xO₅Cl₂:Eu²⁺, Dy³⁺ phosphors display red-shift from 609 nm to 625 nm with increasing B³⁺ concentrations. The XRD patterns show that Al³⁺ can be replaced by B³⁺ in the host lattice at the tetrahedral site, which causes lattice contraction and crystal field enhancement, and thereafter achieves the red-shift on the emission spectrum. The XPS investigation provides direct evidence of the dominant 2-valent europium in the phosphor, which can be ascribed for the broad band emission of the prepared phosphors. The afterglow of all phosphors show standard double exponential decay behavior, and the afterglow of Sr₃Al₂O₅Cl₂:Eu²⁺, Dy³⁺is rather weak, while the sample co-doped with B³⁺shows longer and stronger afterglow, as confirmed after the curve simulation. The analysis of thermally stimulated luminescence showed that, when B³⁺ is introduced, a much deeper trap is created, and the density of the electron trap is also significantly increased. As a result, B³⁺ ions caused redshift and enhanced afterglow for the Sr₃Al_2-xB_xO₅Cl₂:Eu²⁺, Dy³⁺ phosphor.     

High ultraviolet transparent conducting electrodes formed using tantalum oxide/Ag multilayer

We investigated the optical and electrical properties of Ta₂O₅/Ag/Ta₂O₅ films as functions of the thicknesses of the Ta₂O₅ and Ag layers. It was found that with an increase in the thicknesses of the Ta₂O₅ and Ag layers from 10 to 40 nm and from 12 to 24 nm, respectively, the sheet resistance, carrier concentration, electron mobility, and resistivity of the Ta₂O₅/Ag/Ta₂O₅ film varied from 2.02 to 8.95 Ω/sq, 5.74 × 10²¹ to 2.92 × 10²² cm^–3, from 13.21 to 24.07 cm²/V·s, and from 8.89 × 10^-6 to 8.24 × 10^-5 Ω cm, respectively. The average transmittance (T_av) of the multilayer samples ranged from 57.18% to 93.99%, and it depended on the Ta₂O₅ and Ag layer thicknesses. The highest T_av of 93.99% was observed for the film with 35 nm thick Ta₂O₅ and 18 nm thick Ag layers, and the peak Haacke's figure of merit (157.04 × 10^–3 Ω^–1) was obtained for 20 nm thick Ta₂O₅ and 21 nm thick Ag layers. Ta₂O₅ (100 nm) and Ta₂O₅/Ag/Ta₂O₅ (20 nm/21 nm/20 nm) samples had optical bandgaps of 4.70 and 4.45 eV, respectively. Film Wizard simulations were conducted to understand the dependence of the transmittance of the multilayer on the thicknesses of the Ta₂O₅ and Ag layers, and phasor analyses were performed to determine how the transmittance of the Ta₂O₅/Ag/Ta₂O₅ (20 nm/21 nm/20 nm) film depended on the Ta₂O₅ layer's thickness.     

Effects of Electromagnetic Waves with LTE and 5G Bandwidth on the Skin Pigmentation In Vitro

With the rapid growth of wireless communication devices, the influences of electromagnetic fields (EMF) on human health are gathering increasing attention. Since the skin is the largest organ of the body and is located at the outermost layer, it is considered a major target for the health effects of EMF. Skin pigmentation represents one of the most frequent symptoms caused by various non-ionizing radiations, including ultraviolet radiation, blue light, infrared, and extremely low frequency (ELF). Here, we investigated the effects of EMFs with long-term evolution (LTE, 1.762 GHz) and 5G (28 GHz) bandwidth on skin pigmentation in vitro. Murine and Human melanoma cells (B16F10 and MNT-1) were exposed to either LTE or 5G for 4 h per day, which is considered the upper bound of average smartphone use time. It was shown that neither LTE nor 5G exposure induced significant effects on cell viability or pigmentation. The dendrites of MNT-1 were neither lengthened nor regressed after EMF exposure. Skin pigmentation effects of EMFs were further examined in the human keratinocyte cell line (MNT-1-HaCaT) co-culture system, which confirmed the absence of significant hyper-pigmentation effects of LTE and 5G EMFs. Lastly, MelanoDerm™, a 3D pigmented human epidermis model, was irradiated with LTE (1.762 GHz) or 5G (28 GHz), and image analysis and special staining were performed. No changes in the brightness of MelanoDerm™ tissues were observed in LTE- or 5G-exposed tissues, except for only minimal changes in the size of melanocytes. Collectively, these results imply that exposure to LTE and 5G EMFs may not affect melanin synthesis or skin pigmentation under normal smartphone use condition.     

5G多频协同技术研究

与4G时代网络能力主要以下行为主不同,5G时代的业务特性正在发生变化。随着直播业务的高清化,无线视频监控类应用等一系列5G基础业务的兴起,对上行能力提出了更高的要求。本文结合中国移动5G频率资源现状,简述基于5G R16版本的几种多频协同技术,深入分析多频协同技术的网络性能及组网中面临的问题,并提出在实际组网中多频协同技术的选择策略建议,充分发挥各频段的优势,取长补短增强上行能力,最大化资源效率,打造差异化的竞争优势。     

On the application of remote sensing towards the estimation of cultivated land lost to urbanization

In this research work, a 40-km² SPOT-5 High-Resolution Imagery (HRI) of the Warsak locality in district Peshawar, Pakistan, was utilized to approximate the quantity of cultivated land lost to urbanization, due to the construction of new homes and buildings. The imagery from a period of 2005 to 2015 for wheat crop was taken, specifically during the months of March and June when the crop is rich green and golden ripe respectively. eCognition ® program’s Object-Oriented Classification Method (OOCM) was employed for recognition of land versus buildings. Nearest Neighbour (NN), Support Vector Machine (SVM), Decision Trees (DT) and Random Forests (RF) were utilized for the classification process. The results demonstrated that the urbanized area had increased by approximately 28 per cent in the area considered. Moreover, the efficacy of the proposed method is depicted by an accuracy of 97.9 per cent and a Kappa Statistics of 0.975 for the SVM classifier.     

Improved Corrosion Resistance of Selective Laser Melted Ti–5Cu Alloy Using Atomized Ti–5Cu Powder

The different types of metal powder used for selective laser melting(SLM) process would cause distinct corrosion behavior due to the uniformity of the obtained microstructure.The SLM-produced Ti–5Cu alloy using atomized Ti–5Cu metal powder(SLMed Ti–5Cu) in this work reveals a relatively uniform microstructure with overwhelming acicular α/α′ phase and shows great advantages on corrosion resistance compared with the SLM-produced Ti–5Cu alloy using the mixture powder(SLMedM Ti–5Cu).The effect of the micro-galvanic cells decreases due to the undetectable Ti_2Cu phase in the microstructure of the SLMed Ti–5Cu.An apparent passivation behavior was observed for SLMed Ti–5Cu instead of severe pitting phenomenon for the SLMed-M Ti–5Cu.The charge transfer resistance of SLMed Ti–5Cu in this work is 10.09 ± 2.63 MΩ cm~2, which is significantly higher than that of SLMed-M Ti–5Cu(4.76 MΩ cm~2).The above result indicates the atomized Ti–5Cu powder plays an important role in the formation of the uniform microstructure of SLMed product, thereby enhancing its corrosion resistance in Hank's solution at 37 ℃.     

多运营商高铁隧道移动网络覆盖方案

高铁移动网络覆盖是国内三大通信运营商的一个重点,而高铁隧道内移动网络覆盖更是运营商的一大难点痛点。文章根据我国中部省份某高铁线路覆盖规划实例,采用“设备+POI+泄漏电缆”模式,即三家运营商信号源设备通过同一POI(point of interface,多系统接入平台)接入,信号输出到泄漏电缆进行隧道覆盖,隧道口场坪站安装宽频切换天线对隧道外进行延伸覆盖,通过链路预算合理布置各运营商主设备信号源,从而实现隧道到室外的无缝覆盖。最后,根据已有成熟网络覆盖解决方案,对未来5G高铁隧道移动网络覆盖方案进行了探讨。     

阿立哌唑联合草酸艾司西酞普兰对重度抑郁患者多巴胺、5-羟色胺和皮质醇的影响研究

目的:探讨阿立哌唑联合草酸艾司西酞普兰对重度抑郁患者多巴胺（DA）、5-羟色胺（5-HT）和皮质醇的影响。方法:将94例重度抑郁症患者分为观察组和对照组。对照组采用草酸艾司西酞普兰治疗。观察组采用阿立哌唑联合草酸艾司西酞普兰治疗。采用汉密尔顿焦虑量表（HAMD-17）评估患者的抑郁状态。检测两组患者治疗前后DA、5-HT、皮质醇的水平。采用健康状况问卷（SF-36）评估患者的生存质量，包括生理功能、躯体疼痛、生理职能、精神健康、情感职能、生命活力、社会功能、总体健康。观察两组患者治疗过程中不良反应的发生情况。结果:观察组的有效率比对照组高，差异有统计学意义（P<0.05）；两组治疗后的皮质醇、HAMD-17评分显著降低，DA、5-HT显著升高，差异有统计学意义（P<0.05）；观察组治疗后的皮质醇、HAMD-17评分低于对照组，DA、5-HT高于对照组，差异有统计学意义（P<0.05）；两组治疗后的生理功能、躯体疼痛、生理职能、精神健康、情感职能、生命活力、社会功能、总体健康显著升高，差异有统计学意义（P<0.05）；观察组治疗后的生理功能、躯体疼痛、生理职能、精神健康、情感职能、生命活力、社会功能、总体健康高于对照组，差异有统计学意义（P<0.05）。两组的不良反应发生率差异无统计学意义（P>0.05）。结论:阿立哌唑可进一步调节重度抑郁患者DA、5-HT和皮质醇的水平，提高临床疗效，改善生存质量。