特种工程胎硫化外胎质量缺陷原因分析以及解决措施

我公司使用的硫化机采用氮气定型,二次水硫化的工艺。由于特种工程胎胎体偏厚且大,成型机都比较大,生产过程中的帘布筒、钢丝、胎侧等部件都比较重且大。成型胎面使用胎面缠绕机缠绕,所以整个生产过程难度较大。特种工程胎硫化外胎的主要质量问题有:胎面皮泡、胎侧缺胶、胎侧泡、胎肩侧皮泡、子口内侧露线、子口缺胶、胎肚内缺(窝气)、胎肚露线、胎肚皮泡、胎肚串泡、子口支边、胎冠支边、子口鼓包、外胎花缺等。通过对特种工程胎硫化外胎质量缺陷原因分析,找到相应的解决措施,从而减少硫化外胎质量缺陷。     

Mechanisms of Reciprocal Regulation of Gonadotropin-Releasing Hormone (GnRH)-Producing and Immune Systems: The Role of GnRH,Cytokines and Their Receptors in Early Ontogenesis in Normal and Pathological Conditions

Different aspects of the reciprocal regulatory influence on the development of gonadotropin-releasing hormone (GnRH)-producing- and immune systems in the perinatal ontogenesis and their functioning in adults in normal and pathological conditions are discussed. The influence of GnRH on the development of the immune system, on the one hand, and the influence of proinflammatory cytokines on the development of the hypothalamic-pituitary-gonadal system, on the other hand, and their functioning in adult offspring are analyzed. We have focused on the effects of GnRH on the formation and functional activity of the thymus, as the central organ of the immune system, in the perinatal period. The main mechanisms of reciprocal regulation of these systems are discussed. The reproductive health of an individual is programmed by the establishment and development of physiological systems during critical periods. Regulatory epigenetic mechanisms of development are not strictly genetically controlled. These processes are characterized by a high sensitivity to various regulatory factors, which provides possible corrections for disorders.     

往复活塞式压缩机活塞杆温度高故障分析及改造

针对2台2D40型空气压缩机在实际试机过程中,活塞杆温度过高,导致连锁报警,压缩机不能正常工作的问题进行了详细的故障原因分析;对压缩机技术性能参数进行介绍;对压缩机的损伤情况进行说明并找到改造方案,解决机组运行中活塞杆温度过高的问题,从而提高机组的运行效率,使机组运行安全、稳定。     

一种低载噪比突发扩频信号的快速捕获方法实现

针对突发扩频信号用户资源扩大、终端功率降低、系统容量提升等需求,提出了一种低载噪比突发扩频信号的快速捕获硬件实现方法。采用分段匹配滤波器加多普勒并行相干积累的方法,基于硬件实现从算法到工程进行全流程优化设计,最终实现最优的捕获性能。应用结果表明,该硬件设计实现方案的快速捕获性能优越,设计方案正确、可行,已成功应用于工程建设中。     

A Feature Extraction Method for scRNA-seq Processing and Its Application on COVID-19 Data Analysis

Single-cell RNA-sequencing (scRNA-seq) is a rapidly increasing research area in biomedical signal processing. However, the high complexity of single-cell data makes efficient and accurate analysis difficult. To improve the performance of single-cell RNA data processing, two single-cell features calculation method and corresponding dual-input neural network structures are proposed. In this feature extraction and fusion scheme, the features at the cluster level are extracted by hierarchical clustering and differential gene analysis, and the features at the cell level are extracted by the calculation of gene frequency and cross cell frequency. Our experiments on COVID-19 data demonstrate that the combined use of these two feature achieves great results and high robustness for classification tasks.     

基于卷积神经网络的5G蜂窝网络无线定位方法

5G蜂窝网络发展迅猛，其覆盖面积将逐渐增大，因此使用5G蜂窝网络进行定位是有研究潜力的研究方向。本文提出一种新的深度学习技术来实现高效、高精度和低占用的定位，以代替传统指纹定位过程中繁重的指纹库生成以及距离计算。该方法建立了一个特殊的卷积神经网络，并根据5G天线信号的接收信号强度指示、相位和到达角等特征量，选择合适的输入数据格式构造样本组建训练集，对该卷积神经网络进行训练。训练得到的卷积神经网络可以替代指纹定位中的庞大指纹库，非常有利于直接在5G移动设备端实现定位。虽然卷积神经网络在训练过程中需要大量时间，但在训练完毕后直接进行分类定位的速度非常快，可以保障定位实现的实时性。本文所实现的卷积神经网络权重与偏置所占内存不到0.5 MB，且能够在实际应用环境中以95%的定位准确率以及0.1 m的平均定位精度实现高精度定位。     

小微数据包高效无线传输技术的发展和趋势

5G-NR和B5G系统都需能高效地支持小微数据包(块)的传输,且在不同的无线传输技术(如宽带或超低时延)之间快速灵活地切换。系统梳理和比较和小微数据包(块)相关的多种无线传输技术,如两步RACH、预配置授权和公共资源竞争等,分析它们对3GPP各个系统和各个RAN协议的影响和复杂度优劣等,并进一步展望其未来发展和应用趋势。     

Update on the Roles of Rice MAPK Cascades

The mitogen-activated protein kinase (MAPK) cascades have been validated playing critical roles in diverse aspects of plant biology, from growth and developmental regulation, biotic and abiotic stress responses, to phytohormone signal transduction or responses. A classical MAPK cascade consists of a MAPK kinase kinase (MAPKKK), a MAPK kinase (MAPKK), and a MAPK. From the 75 MAPKKKs, eight MAPKKs, and 15 MAPKs of rice, a number of them have been functionally deciphered. Here, we update recent advances in knowledge of the roles of rice MAPK cascades, including their components and complicated action modes, their diversified functions controlling rice growth and developmental responses, coordinating resistance to biotic and abiotic stress, and conducting phytohormone signal transduction. Moreover, we summarize several complete MAPK cascades that harbor OsMAPKKK-OsMAPKK-OsMAPK, their interaction with different upstream components and their phosphorylation of diverse downstream substrates to fulfill their multiple roles. Furthermore, we state a comparison of networks of rice MAPK cascades from signal transduction crosstalk to the precise selection of downstream substrates. Additionally, we discuss putative concerns for elucidating the underlying molecular mechanisms and molecular functions of rice MAPK cascades in the future.     

Human DDX17 Unwinds Rift Valley Fever Virus Non-Coding RNAs

Rift Valley fever virus (RVFV) is a mosquito-transmitted virus from the Bunyaviridae family that causes high rates of mortality and morbidity in humans and ruminant animals. Previous studies indicated that DEAD-box helicase 17 (DDX17) restricts RVFV replication by recognizing two primary non-coding RNAs in the S-segment of the genome: the intergenic region (IGR) and 5′ non-coding region (NCR). However, we lack molecular insights into the direct binding of DDX17 with RVFV non-coding RNAs and information on the unwinding of both non-coding RNAs by DDX17. Therefore, we performed an extensive biophysical analysis of the DDX17 helicase domain (DDX17_135–555) and RVFV non-coding RNAs, IGR and 5’ NCR. The homogeneity studies using analytical ultracentrifugation indicated that DDX17_135–555, IGR, and 5’ NCR are pure. Next, we performed small-angle X-ray scattering (SAXS) experiments, which suggested that DDX17 and both RNAs are homogenous as well. SAXS analysis also demonstrated that DDX17 is globular to an extent, whereas the RNAs adopt an extended conformation in solution. Subsequently, microscale thermophoresis (MST) experiments were performed to investigate the direct binding of DDX17 to the non-coding RNAs. The MST experiments demonstrated that DDX17 binds with the IGR and 5’ NCR with a dissociation constant of 5.77 ± 0.15 µM and 9.85 ± 0.11 µM, respectively. As DDX17_135–555 is an RNA helicase, we next determined if it could unwind IGR and NCR. We developed a helicase assay using MST and fluorescently-labeled oligos, which suggested DDX17_135–555 can unwind both RNAs. Overall, our study provides direct evidence of DDX17_135–555 interacting with and unwinding RVFV non-coding regions.     

Recent Advances in Preclinical Research Using PAMAM Dendrimers for Cancer Gene Therapy

Carriers of genetic material are divided into vectors of viral and non-viral origin. Viral carriers are already successfully used in experimental gene therapies, but despite advantages such as their high transfection efficiency and the wide knowledge of their practical potential, the remaining disadvantages, namely, their low capacity and complex manufacturing process, based on biological systems, are major limitations prior to their broad implementation in the clinical setting. The application of non-viral carriers in gene therapy is one of the available approaches. Poly(amidoamine) (PAMAM) dendrimers are repetitively branched, three-dimensional molecules, made of amide and amine subunits, possessing unique physiochemical properties. Surface and internal modifications improve their physicochemical properties, enabling the increase in cellular specificity and transfection efficiency and a reduction in cytotoxicity toward healthy cells. During the last 10 years of research on PAMAM dendrimers, three modification strategies have commonly been used: (1) surface modification with functional groups; (2) hybrid vector formation; (3) creation of supramolecular self-assemblies. This review describes and summarizes recent studies exploring the development of PAMAM dendrimers in anticancer gene therapies, evaluating the advantages and disadvantages of the modification approaches and the nanomedicine regulatory issues preventing their translation into the clinical setting, and highlighting important areas for further development and possible steps that seem promising in terms of development of PAMAM as a carrier of genetic material.