氮酮与薄荷醇联用对5-氟尿嘧啶的促透作用

目的：以5-氟尿嘧啶（5-FU）为模型药物，对氮酮和薄荷醇的促透特性进行比较，并探讨两种促透剂合用时的促透效果。方法：在离体透皮实验装置上进行透皮试验，由浓度计算累积透过量，运用倍量法对氮酮与薄荷醇联用的效果进行评价。结果： 浓度为0.25%、0.50%、1.00%、2.00%的氮酮对5-FU均有促透作用，与空白对照组相比，差异具有统计学意义（P<0.01）；浓度为0.25%、0.50%、1.00%、2.00%的薄荷醇对5-FU也有明显的促透作用，与空白对照相比，差异具有统计学意义（P<0.01）；用倍量法对两种药物联用的作用进行评价分析时，二者联用未显示有协同作用。结论：氮酮和薄荷醇对（5-FU）均有促透作用，但二者以相同浓度合用时，未有明显的协同作用。     

气相色谱法测定薄荷醇微胶囊的包裹率

用超临界流体微粒化技术制备薄荷醇的微胶囊,气相色谱法分别测定微胶囊表面薄荷醇和总薄荷醇的含量,并以此计算包裹率.结果表明,用乙醇和乙醚为溶剂,薄荷醇在0.025～0.800 mg/mL浓度范围内与色谱积分面积有很好的线性关系,相关系数均接近1;所用壁材三棕榈酸甘油酯对结果没有影响.用乙醇为溶剂,薄荷醇平均回收率为100...     

薄荷烷基氯制备研究

由于国内未见左旋薄荷烷基氯成品的研究报道,为此本文采用lucas试剂法,以左旋薄荷醇为原料对左旋薄荷烷基氯进行了合成研究。研究结果显示,采用该方法制备的左旋薄荷烷基氯产品为无色无味的透明液体,沸点,收率84.82%,比旋光度[α]D20=-44.49°。把该方法制得的薄荷烷基氯,用于新型生理清凉剂N-乙基-对-薄荷烷-3-羧酰胺的合成实验,取得了良好效果。     

气相色谱法测定牙膏中的薄荷醇量

采用毛细管气相色谱法测定牙膏中薄荷醇的量。该方法的线性相关系数为：1．000；平均精密度为：1％；平均回收率为：100．7％。实验结果显示：该测定方法准确、稳定，能准确定量不同类型牙膏中的薄荷醇量，同时该测量定方法简便，可操作性强，因此可以作为牙膏产品质量监控的方法之一，也可为牙膏新产品研发提供有效的参考依据。同时，实验结果还表明，不同类型及不同品牌牙膏中的薄荷醇量有着显的差别。     

薄荷醇促透皮吸收作用的研究进展

对近年关于薄荷醇促透皮吸收方面的研究进展作一综述。     

凝聚法制备薄荷脑微胶囊技术研究

研究了凝聚法制备薄荷脑微胶囊的工艺过程和方法。采用海藻酸钠和明胶为壁材，通过正交实验，确定了微胶囊化的较佳工艺条件。即海藻酸钠浓度溶液1％，明胶溶液浓度6％，pH值为4．1，温度40℃。薄荷脑用量为1．0g左右。此条件下制备的微胶囊效果最佳。     

不同金属离子改性的硅酸铝对薄荷醇吸附/脱附性能研究

本文应用浸渍法制备三种改性的硅酸铝(AS)材料Fe3+/AS、Cu2+/AS、Ag+/AS,采用静态吸附法测定了改性硅酸铝对薄荷醇的吸附量;应用程序升温脱附实验测定了薄荷醇在改性硅酸铝上的脱附活化能,并应用HSAB软硬酸碱理论分析和讨论了薄荷醇在不同改性硅酸铝上脱附活化能的差异。结果表明:与原始硅酸铝相比,金属离子改性后硅酸铝比表面积显著增加,增幅依次为105.83%、44.41%、32.69%。改性材料对薄荷醇吸附量增加,吸附量顺序为Fe3+/ASCu2+/ASAg+/ASAS。此外,该改性改变了薄荷醇与硅酸铝表面的结合力,薄荷醇在改性硅酸铝上的脱附活化能顺序如下:Ag+/ASCu2+/ASFe3+/AS。与原始硅酸铝相比,Ag+和Cu2+增强了薄荷醇与硅酸铝之间的结合力,Fe3+减弱了薄荷醇与硅酸铝之间的结合力,与HSAB软硬酸碱理论预测的结果一致。     

Biotransformation of l‐menthol by twelve isolates of soil‐borne plant pathogenic fungi (Rhizoctonia solani) and classification of fungi

The microbial transformation of l‐menthol ( 1 ) was investigated by using 12 isolates of soil‐borne plant pathogenic fungi, Rhizoctonia solani (AG‐1‐IA Rs24, Joichi‐2, RRG97‐1; AG‐1‐IB TR22, R147, 110.4; AG‐1‐IC F‐1, F‐4, P‐1; AG‐1‐ID RCP‐1, RCP‐3, and RCP‐7) as a biocatalyst. Rhizoctonia solani F‐1, F‐4 and P‐1 showed 89.7–99.9% yields of converted product from 1 , RCP‐1, RCP‐3, and RCP‐7 26.0–26.9% and the other isolates 0.1–12.0%. In the cases of F‐1, F‐4 and P‐1, substrate 1 was converted to (?)‐(1S,3R,4S,6S)‐6‐hydroxymenthol ( 2 ), (?)‐(1S,3R,4S)‐1‐hydroxymenthol ( 3 ) and (+)‐(1S,3R,4R,6S)‐6,8‐dihydroxymenthol ( 4 ), which was a new compound. Substrate 1 was converted to 2 and/or 3 by RRG97‐1, 110.4, RCP‐1, RCP‐3 and RCP‐7. The structures of the metabolic products were elucidated on the basis of their spectral data. In addition, metabolic pathways of the biotransformation of 1 by Rhizoctonia solani are discussed. Finally, from the main component analysis and the differences in the yields of converted product from 1 , the 12 isolates of Rhizoctonia solani were divided into three groups based on an analysis of the metabolites. Copyright © 2003 Society of Chemical Industry     

Exploring the anticancer properties of essential oils from family Lamiaceae

Lamiaceae is among the largest families of flowering plants with about 250 genera and over 7,000 species distributed around the world. It is considered as the important source of essential oils, for example, menthol, geraniol, eucalyptol, camphor and thymol. Therefore, it is imperative to study these economically important compounds under in vitro conditions for their sustainable and enhanced production. In addition to proven biological activities, essential oils from this family have recently been evaluated for anticancer activities and considered as a source of anticancer drugs. Mechanisms involved in the essential oils-mediated antiproliferative activity include cell cycle arrest, apoptosis and DNA repair mechanisms. Essential oils also act in the reduction of tumors, inhibiting metastasis and as anti-multidrug resistance molecules. The aim of this review is to assess the anticancer properties of essential oils obtained from different members of family Lamiaceae. The available reports on active components of essential oils and their effect on cancer type and cell line have been discussed. Biotechnological studies to improve the production of essential oils have also been highlighted. Various methods have been adopted to obtain essential oils under in vitro conditions from different plant species of family Lamiaceae, and their production is affected by culture conditions, cultivation mode, utilization of nutrient media and plant growth regulators. The literature survey suggests that essential oils obtained from family Lamiaceae have perspective for the development of new alternatives for disease treatment and prevention.