Split Enzyme-Based Biosensors for Structural Characterization of Soluble and Insoluble β-Glucans

β-Glucan is widely distributed in various plants and microorganisms and is composed of β-1,3-linked d-glucose units. It may have a branched short or long side chain of glucose units with β-1,6- or β-1,4-linkage. Numerous studies have investigated different β-glucans and revealed their bioactivities. To understand the structure-function relationship of β-glucan, we constructed a split-luciferase complementation assay for the structural analysis of long-chain β-1,6-branched β-1,3-glucan. The N- and C-terminal fragments of luciferase from deep-sea shrimp were fused to insect-derived β-1,3-glucan recognition protein and fungal endo-β-1,6-glucanase (Neg1)-derived β-1,6-glucan recognition protein, respectively. In this approach, two β-glucan recognition proteins bound to β-glucan molecules come into close proximity, resulting in the assembly of the full-length reporter enzyme and induction of transient luciferase activity, indicative of the structure of β-glucan. To test the applicability of this assay, β-glucan and two β-glucan recognition proteins were mixed, resulting in an increase in the luminescence intensity in a β-1,3-glucan with a long polymer of β-1,6-glucan in a dose-dependent manner. This simple test also allows the monitoring of real-time changes in the side chain structure and serves as a convenient method to distinguish between β-1,3-glucan and long-chain β-1,6-branched β-1,3-glucan in various soluble and insoluble β-glucans.     

我国水-能源-粮食耦合关系及影响因素

为缓解我国水、能源和粮食资源紧张问题，促进资源可持续利用，构建水-能源-粮食系统，利用耦合协调度模型对我国的30个省（自治区、直辖市）进行测算,并利用空间杜宾模型分析主要影响因素。结果表明：2003—2017年，我国能源、粮食评价[JP]指数高于水资源评价指数，系统综合评价指数逐年递增；大部分省份耦合协调度处于初级协调水平且呈现逐年上升的态势，个别省份耦合协调度濒临失调；耦合协调度空间自相关性较强，虽有明显波动，但是呈现逐年加强的态势；影响耦合协调度的主要因素有从业人口数、固定资产投资额、人均生产总值、人口总数、[JP]文盲人口占比、工业污染排放、城镇化。     

基于音频特征的工程车辆工况识别研究

高效率地使用工程车辆是工程项目管理中节约成本的有效方法，无人监管环境下工程车辆的工况识别，是实现工程车辆高效率使用的有效手段。目前以GPS等技术为核心的车辆智能管理系统未对工程车辆进行工况识别，提出一种基于GRU循环神经网络的工程车辆工况识别方法，通过对工程车辆在不同工况下产生的音频信号进行分析，从中提取Mel倒谱系数作为主要特征，构建GRU循环神经网络模型进行训练和识别。实验结果表明，该方法可以实现对工程车辆工况的有效识别。     

空间曲线的特征识别与高质量非均匀采样

为避免传统均匀采样方法因忽视曲线重要特征而生成不理想的采样结果,获得给定数量且由特征点和辅助点组成的采样点序列,提出基于特征识别的高质量空间曲线非均匀采样方法.首先使用抛物线插值法得到曲线上所有曲率极大值点和挠率极大值点的近似位置,经筛选后产生特征点,以更好地抓住空间曲线的轮廓特征.然后定义基于弧长、曲率和挠率加权组合的特征函数,并以此自适应地选取曲线上的辅助点.与3种主流采样方法比较的实验结果表明,该方法能够获得更高质量的采样结果且具有更好的实用性,从而进一步改善空间曲线的B样条拟合效果.     

国际橄榄理事会的定位和使命及相关标准（网络首发、推荐阅读）

The International Olive Council(IOC) is an international intergovernmental organization dedicated to olive oil and table olives, aiming at modernizing olive production, coordinating olive policies, improving the regulation of international trade, defending the quality of the olive sector and promoting olive oil and table olives to increase their consumption. The IOC grants recognition of laboratories and tasting panels in annual trials when they meet the conditions given in the decisions adopting the IOC certificate for laboratories for the physico–chemical testing of olive oil and laboratories for the sensory analysis of virgin olive oils. The IOC establishes analysis methods applying to olive oils and olive pomace oils for purity, quality and organoleptic assessment. The IOC elaborates guides of storage conditions for olive oils and olive pomace oils, of managing virgin olive oil tasting panels and of sensory testing laboratories. In future works, the IOC includes activities to identify analytical criteria for detecting fraud and guaranteeing the quality of olive oils and olive pomace oils.     

Optimization of hydrogen enrichment via palladium membrane in vacuum environments using Taguchi method and normalized regression analysis

In this study, the separation of hydrogen from gas mixtures using a palladium membrane coupled with a vacuum environment on the permeate side was studied experimentally. The gas mixtures composed of H₂, N₂, and CO₂ were used as the feed. Hydrogen permeation fluxes were measured with membrane operating temperature in the range of 320–380 °C, pressures on the retentate side in the range of 2–5 atm, and vacuum pressures on the permeate side in the range of 15–51 kPa. The Taguchi method was used to design the operating conditions for the experiments based on an orthogonal array. Using the measured H₂ permeation fluxes from the Taguchi approach, the stepwise regression analysis was also employed for establishing the prediction models of H₂ permeation flux, followed by the analysis of variance (ANOVA) to identify the significance and suitability of operating conditions. Based on both the Taguchi approach and ANOVA, the H₂ permeation flux was mostly affected by the gas mixture composition, followed by the retentate side pressure, the vacuum degree, and the membrane temperature. The predicted optimal operating conditions were the gas mixture with 75% H₂ and 25% N₂, the membrane temperature of 320 °C, the retentate side pressure of 5 atm, and the vacuum degree of 51 kPa. Under these conditions, the H₂ permeation flux was 0.185 mol s^?1 m^?2. A second-order normalized regression model with a relative error of less than 7% was obtained based on the measured H₂ permeation flux.     

针对细微特征进行K-means聚类的电台分选识别技术

现代战场中的无线通信设备日益增多，精准获取个体信息已成为研究热点，但也是难点。针对通信电台，提出了一种分选识别技术。该技术从电台物理层特性出发，对其辐射信号的细微特征进行K-means聚类以实现分选，分选的同时提取各个个体的特征属性值，未知信号通过与特征属性值相关运算实现个体识别。该技术无需先验知识，无需训练运算，通过实验验证，其可行、高效，易于工程实现。     

智能假肢膝关节的研发要点及其研究进展综述

目的 介绍国内外智能假肢膝关节研究的主要进展,为相关领域的研发提供可借鉴的思路.方法 基于机械结构、调控方式、驱动方式,对典型假肢膝关节进行了分类比较,并从假肢穿戴者的运动意图识别、驱动控制、人机协调控制等方面做了分析.此外,对假肢膝关节在智能化研究与安全性、个性化与通用性、人机共融技术、科学伦理、关键技术等方面需要注意的问题,提出了研发要点和技术思路.结论 智能假肢膝关节应注重安全性与稳定性,规避用户在使用过程中的潜在危险因素;实现假肢控制参数的自整定和灵活适配,在个性化与通用性上达到平衡;综合考虑人—机—环境因素,实现协调控制;坚持"以人为本",在技术方法和科学伦理两个方面开展医工结合的研究;突破关键技术限制,建立完善的社会医疗康复保障服务体系.