Novel fluorescence sensor based on covalent immobilization of 3-amino-9-ethylcarbazole via electrostatically assembled gold nanoparticle layer

A novel technique of immobilizing indicator dyes by electrostatic adsorption and covalent bonding to fabricate optical sensors was developed. 3-Amino-9-ethylcarbazole (AEC) was attached to the outmost surface of quartz glass slide via aminosilanizing the slide, crosslinking chitosan, adsorbing Au nanoparticle, self-assembling HS(CH₂)₁₁OH, and coupling AEC. Thus, an AEC-immobilized optical sensor was obtained. The sensor exhibits a wide linear response range from 7.0×10⁻⁷ to 1.0×10⁻⁴ mol/L and a correlation coefficient of 0.995 9 for the detection of 2-nitrophenol. The detection limit and response time of the sensor are 1.0×10⁻⁷ mol/L and less than 10 s, respectively. The fluorescence intensity of the used sensor can be restored to the blank value by simply rinsing with blank buffer. A very effective matrix for immobilizing indicator dye is provided by the proposed technique, which is adaptable to other indicator dyes with amino groups besides AEC. Foundation item: Project(20775010) supported by the National Natural Science Foundation of China; Project(208095) supported by the Key Project of Ministry of Education, China; Project(07A006) supported by the Scientific Research Fund of Hunan Provincial Education Department, China; Project(07JJ3020) supported by Hunan Provincial Natural Science Foundation of China     

掺杂1-萘硼酸的荧光纳米颗粒的制备及其在奥硝唑测定中的应用

采用水包油微乳液聚合法制备了一种掺杂1-萘硼酸的荧光纳米颗粒,并用于奥硝唑的测定。当荧光纳米颗粒的浓度固定为0.2 mg/mL时,对奥硝唑的线性响应范围为8.0×10-7~8.0×10-5mol/L,检测下限为5.0×10-7mol/L。掺杂1-萘硼酸的荧光纳米颗粒的耐光漂白性与游离萘硼酸的耐光漂白性相比有一定程度的提高。     

EM>N-/EM>琥珀酰亚胺-3-碘［S

研究能有效降低抗体的体内脱碘的标记方法。碘标记N-琥珀酰亚胺-3-(三正丁基锡)苯甲酸酯(ATE)前体,得到N-琥珀酰亚胺-3-碘[125I]苯甲酸酯(S125IB),分别与人IgG和抗人肝癌单抗(Hepama-1)进行偶联,探索最佳标记条件,并测定标记物的稳定性和生物活性,研究直接标记和间接标记的Hepama-1在正常小鼠体内的生物学分布。结果表明,用N-氯代琥珀酰亚胺(NCS)法125I标记ATE前体,在ATE用量为25～100μg、NCS用量为10～20μg、磷酸盐缓冲溶液(PBS)用量为10～20μL、反应时间为5 min时,标记率大于95%;S125IB和人IgG的偶联率最高可达75%,偶联产物稳定性、生物活性良好;与Hepama-1偶联率可达75%以上。生物分布的对比实验证明,1,3,4,6-四氯-3α,6α-二苯甘脲(Iodogen)直接标记的Hepama-1在甲状腺的放射性摄取率(脱碘显示)最高是S125IB间接标记的Hepama-1的87.9倍。这说明以ATE为前体的放射性碘间接标记蛋白质方法与传统的碘直接标记方法相比较,在解决体内严重脱碘问题上具有明显的优越性。     

fac-[Μ(CO)_3]~+(Μ=~(188/186)Re,~(99)Tc~m)化合物的生物标记及应用

fac-[Μ(CO)_3]~+(Μ=~(188/186)Re,~(99)Tc~m)具有良好的放射生物学特性,是一种很有发展前途的标99记前体。本文综述了fac-[Μ(CO)3]+(Μ=188/186Re,Tcm)核标记生物小分子(生物素、雌激素……)、大分子蛋99白及多肽的研究进展,并探讨了其今后发展的方向。     

非化学化工专业卓越班《大学化学》教学改革与实践

《大学化学》课程是理工科院校非化学化工专业的一门基础课。为了提高卓越班学生的学习积极性和主观能动性,激发学生强烈的求知欲和极大的探索热情,明确学习化学知识的重要作用及意义。根据《大学化学》这门课的内容,对《大学化学》课程的教学模式提出一些改革措施,例如案例分析、探究讨论、合作学习,加强与学生的互动交流等。以期提高学生素质,使学生更符合社会发展的要求。     

PTFE微孔膜透湿性能测试研究

应用透湿杯法测量PTFE微孔膜的透湿率,研究了温度、湿度以及干燥剂量等因素对测试结果的影响。结果表明,在湿度一定的情况下,膜的透湿率随温度升高而提高;在恒定温度下,膜的透湿率随湿度增加而提高;随干燥剂用量增大,膜的透湿率下降。     

fac-[M(CO)3]+(M=188/186Re,99Tcm)化合物的生物标记及应用

fac-[M(CO)3L3]^ (M=^188/186Re,^99Tc^m,L=H2O)具有良好的放射生物学特性,是一种很有发展前途的标记前体。本文综述了fac-[M(CO)3]^ (M=^188/186Re,^99Tc^m)核标记生物小分子(生物素、雌激素……)、大分子蛋白及多肽的研究进展,并探讨了其今后发展的方向。     

Electrochemical synthesis, characterization and thermal properties of niobium ethoxide

Niobium(V) ethoxide (Nb(OEt)₅) was synthesized by electrochemical reaction of ethanol with niobium plate as the sacrificial anode, stainless steel as the cathode and tetraethylammonium chloride (TEAC) as the conductive additive. The condensates were isolated by vacuum distillation under 5 kPa. The product was characterized by Fourier transform infrared (FT-IR) spectra, Raman spectra and nuclear magnetic resonance (NMR) spectra. The results indicate that the product is niobium ethoxide. Thermal properties of niobium ethoxide were analysed by TG/DTG. Vapour pressure was calculated from the Langmuir equation and the enthalpy of vaporization was calculated from the vapour pressure-temperature data using the Clausius-Clapeyron equation. The concentrations of impurity metallic elements in the sample were detected by ICP-MS. It is shown that the purity can reach 99.997%. The volatility and purity of the niobium ethoxide ensure that it could be a good precursor for chemical vapor deposition and atomic layer deposition of niobium oxide layers.     

ICP—MS同时测定水处理剂中的微量元素

为了建立简便、灵敏、准确的测定水处理剂中微量元素的方法，采用电感耦合等离子体质谱法（ICP-MS）对水处理剂聚合氯化铝中的As、Pb和Cd 3种有毒元素进行了分析测定。实验结果表明，测试样品固体聚合氯化铝（稀释100倍）中As、Pb和Cd的测定结果为：     

叶酸在放射性核素标记研究中的应用

叶酸在人体内有重要的生理功能,它能特异性结合过度表达于许多肿瘤（卵巢癌、乳腺癌、直肠癌等）细胞膜表面的叶酸受体.在肿瘤靶向药物输运体系中,包括输运放射性核素标记的叶酸螯合物显像剂,叶酸受体是一种潜在的分子靶.近年来,有关放射性核素标记的叶酸螯合物显像剂的研究越来越多.本文着重介绍了放射性核素^111In、^67Ga和99Tc^m（Re）标记叶酸的螯合物显像剂以及它们在肿瘤诊断方面的临床应用.这些资料表明,99Tc^m标记叶酸的螯合物是一种比较有发展前途的显像剂.