排序方式： 共有3条查询结果，搜索用时 6 毫秒
1
1.
采用十六烷基三甲基溴化铵（CTAB）作为表面活性剂修饰羧基化的单壁碳纳米管（SWNT-COOH/CTAB）,并对原始单壁碳纳米管（SWNTs）与羧基化修饰的单壁碳纳米管（SWNT-COOH）进行材料学特征比较。通过细胞活力和细胞凋亡实验对SWNTs、SWNT-COOH和SWNT-COOH/CTAB的细胞毒性进行比较。结果表明,羧基化修饰的单壁碳纳米管比原始单壁碳纳米管的毒性小,单壁碳纳米管经羧基化后其毒性降低;浓度及时间曲线显示SWNT-COOH/CTAB的毒性与表面活性剂CTAB相关,CTAB和 SWNT-COOH/CTAB的细胞毒性在低浓度范围内（0.5-25μg/mL）是可接受的。十六烷基三甲基溴化铵修饰的羧基化单壁碳纳米管在低浓度范围（0.5-25μg/mL）内可以较安全地用于生物医学领域。 相似文献
2.
应用十六烷基三甲基溴化铵(CTAB)作为表面活性剂修饰单壁碳纳米管,研究CTAB修饰后的单壁碳纳米管的分散情况和表面电荷情况;观察在场发射扫描电子显微镜和透射电子显微镜下的形貌,同时还研究CTAB修饰后的单壁碳纳米管与小干扰RNA结合的最佳浓度配比,以及CTAB功能修饰后的碳纳米管对培养的人脐静脉内皮细胞的毒性。结果表明:CTAB修饰后的单壁碳纳米管分散良好,CTAB吸附到单根或成束的碳纳米管管壁上,表面带正电荷;与带负电荷的寡核苷酸分子小干扰RNA可以结合,并且CTAB-SWNT与小干扰RNA结合比例达到1:1.5到1:2之间时基本饱和;缺乏CTAB的单壁碳纳米管不能结合小干扰RNA;没有分散的单壁碳纳米管具有更大的细胞毒性,CTAB可以改善SWNTs的分散性,从而减轻单壁碳纳米管的细胞毒性。 相似文献
3.
Hydroxyapatite（HA） nanoparticles were prepared by coprecipitation-hydrothermal synthesis and their exosyndrome was estimated via transmission electron microscopy. Agarose gel electrophoresis and ultraviolet spectrophotometry were used to evaluate the ability of HA to bind NR2B-siRNA at different pH values and at different HA：NR2B-siRNA ratios. And the stability of the complex in saline was also evaluated. The effect of HA/NR2B-siRNA complex on chronic inflammatory pain was evaluated in vivo, in mice. Transmission electron microscopy（TEM） reveals that HA nanoparticles are thin strips or short rod in shape and thc one-dimensional particle size of HA nanoparticles is 40-50 nm. Under the acid or neutral condition, the Zeta potential of HA is positive; nanoparticles can completely bind NR2B-siRNA when the HA：NR2B-siRNA ratio is at or larger than 35：1; while under the alkaline condition, the affinity of HA to NR2B-siRNA is rather weak. HA/NR2B-siRNA complex is not dissociated when being resuspended in saline. The nociception of the tonic phase induced by formalin is significantly reduced in the HA：NR2B-siRNA treated mice as compared with the. controls. Therefore, HA may be a new siRNA nano-vector material. 相似文献
1