重组大肠杆菌分批-补料高密度发酵生产类人胶原蛋白的动力学

The kinetics of batch and fed-batch cultures of recombinant Escherichia coli producing human-like collagen was investigated. In the batch culture, a kinetic model of a simple growth-association system was concluded without consideration of cell endogeneous metabolism. The cell lag time, the maximum specific growth rate and growth rates were set at (0.15, 0.2, 0.25h-1) by the method of pseudo-exponential feeding, and the expressions for the specific rate of substrate consumption, the growth kinetics and the product formation kinetics of each phase spectively. The model predictions are in good agreement with the experimental data.     

基因重组大肠杆菌生产类人胶原蛋白补氮策略优化研究

优化重组大肠杆菌高密度发酵的调控工艺.利用重组大肠杆菌高密度发酵生产类人胶原蛋白,考察了连续流加和分阶段式两种不同补氮策略对细胞生长和类人胶原蛋白产量的影响.不同补氮方式显著影响类人胶原蛋白的产量.分阶段式补氮方式优于连续流加补氮方式,有利于细胞生长和类人胶原蛋白的表达,最终细胞浓度为76.01 g·L-1(DCW),类人胶原蛋白浓度为16.75 g·L-1,蛋白表达量为22.1%.     

重组大肠杆菌分批-补料高密度发酵生产类人胶原蛋白的动力学

The kinetics of batch and fed-batch cultures of recombinant Escherichia coli producing human-like collagen was investigated. In the batch culture, a kinetic model of a simple growth-association system was concluded without consideration of cell endogeneous metabolism. The cell lag time, the maximum specific growth rate and Yx/s were determined as 1.75h, 0.65h^-1 and 0.51g·g^-1, respectively. In the fed-batch culture, different specific growth rates were set at （0.15, 0.2, 0.25h^-1） by the method of pseudo-exponential feeding, and the expressions for the specific rate of substrate consumption, the growth kinetics and the product formation kinetics of each phase were obtained. The result shows that the concentrations of cell and product can reach 77.5g·L^-1 and 10.2g·L^-1 respectively. The modal predictions are in good agreement with the experimental data.     

热轧态Al-Li合金的超塑性研究

本研究运用动态再结晶诱发超塑性的原理,对未经细化晶粒预处理的常规生产热轧态2091Al-Li合金直接进行高温拉伸,试验结果表明合金在470～530℃温度范围和2×10~(-4)～1×10~(-3)应变速率范围内具有超塑性,最大断裂延伸率达405％。根据光镜和电镜组织观察和真应力—真应变曲线的单一峰值和变形激活能随应变量增大而下降等特征,讨论了动态再结晶诱发超塑性的机制。     

基于业务流程整合的企业应用集成

本文介绍了信息孤岛的形成,并提出一个解决方案——企业应用集成(EAI)。概述了EAI技术之后,重点介绍了基于业务流程整合的EAI技术。最后说明EAI技术是企业信息化的必然趋势。