酚酸对水产品腐败希瓦氏菌的抑菌作用

比较了没食子酸、原儿茶酸和绿原酸3种酚酸对腐败希瓦氏菌的抑制活性,并通过扫描电镜、流式细胞仪、细胞膜完整性和通透性测定等探讨了没食子酸对腐败希瓦氏菌的抑菌作用。结果表明:3种酚酸中没食子酸对腐败希瓦氏菌的抑制活性最强,其最小抑菌浓度和最小杀菌浓度分别为1.25,2.50mg/mL。经最小抑菌浓度没食子酸处理后,腐败希瓦氏菌细胞形态改变,表面有明显凹陷;细胞凋亡率明显增高;核酸和蛋白泄漏增加,细胞膜完整性遭到破坏;细胞膜通透性增加,胞内电解质大量泄漏至培养液中。

Antibacterial effect of phenolic acid on Shewanella putrefaciens from aquatic product

Compared the antibacterial activities of three phenolic acids (gallic acid, protocatechuic acid, and chlorogenic acid) against S. putrefaciens, and investigated the antibacterial effects of gallic acid on S. putrefaciens by means of scanning electron microscopy, flow cytometry, cell membrane integrity and permeability measurement. The results showed that gallic acid had the strongest antibacterial activity against S. putrefaciens among the three phenolic acids, and its minimal inhibitory concentration and minimal bactericidal concentration were 1.25 mg/mL and 2.5 mg/mL, respectively. After treatment with gallic acid at the minimum inhibitory concentration, the cell morphology of S. putrefaciens changed with obvious depression on the surface. The apoptotic rate increased significantly. The leakage of nucleic acid and protein increased, and the integrity of cell membrane was destroyed. The permeability of cell membrane increased, and a large amount of intracellular electrolytes leaked into the culture medium.