共查询到19条相似文献,搜索用时 78 毫秒
1.
目的通过体外代谢方法,研究五氯苯酚(pentachlorophenol,PCP)的代谢情况。方法采用肝微粒体和肝S9成分2种体外代谢试剂模拟体内代谢模式,通过优化代谢反应条件,建立PCP、四氯苯醌(Cl4BQ)2种目标物的气相色谱-质谱联用(GC-MS)检测方法,对PCP进行体外代谢研究分析。结果体外代谢分析的最佳反应条件为代谢试剂浓度0.75 mg/m L、代谢反应时间4 h。结论在最优反应条件下分析比较2种试剂对PCP的代谢情况,验证了PCP的代谢产物为Cl4BQ,且在肝微粒体和肝S9成分中的代谢没有显著差异。 相似文献
2.
目的通过模拟体内代谢,对双酚A二缩水甘油醚(BADGE)的体外基本代谢情况进行研究。方法采用肝微粒体、肝S9 2种体外代谢试剂,通过模拟体内肝脏代谢,对BADGE的代谢行为及其代谢产物进行研究。通过代谢试剂浓度及代谢时间条件的优化,采用高效液相色谱-串联质谱(HPLC-MS/MS)作为检测手段对BADGE的体外代谢产物进行分析确证。结果体外代谢最佳孵化时间为60 min,最佳体外代谢试剂浓度为0.5mg/m L,在肝S9及肝微粒体2种体外代谢试剂的作用下,BADGE发生显著的代谢反应。结论本研究与传统的动物试验相比,节约了时间、精力,对食品包装材料的毒理学研究和安全性评价有重要的推动作用。 相似文献
3.
利用气相色谱-质谱(gas chromatography-mass spectrometry,GC-MS)分离鉴定不同种类绿豆(大明绿豆和九鲤湖绿豆)中的代谢产物。以代谢组学研究方法为基础,以绿豆为研究对象,先利用甲醇溶液提取极性代谢组分,再用N,O-双(三甲基硅烷基)三氟乙酰胺进行衍生化处理,通过GC-MS进行代谢产物的分离鉴定。结果表明:分离鉴定共得到2?个品种绿豆的总代谢物67?种,其中相同代谢产物30?种,但含量均有不同。大明绿豆相对于九鲤湖绿豆的差异代谢物有10?种,九鲤湖绿豆相较于大明绿豆的差异代谢物有20?种,并且九鲤湖绿豆的糖代谢途径、脂肪酸代谢途径、氨基酸代谢途径、三羧酸循环都比大明绿豆代谢活跃;在大明绿豆中,较为优势代谢产物(>7%)为L-正缬氨酸、肌醇2?种,在九鲤湖绿豆中,较为优势代谢产物仅为L-正缬氨酸。这种代谢产物差异性受不同品种的基因差异性、代谢途径和机制差异性等因素的综合影响。 相似文献
4.
基于气相色谱-质谱(gas chromatography-mass spectrometry,GC-MS)代谢组学方法研究同一产地不同品种小米的代谢产物。用甲醇溶液提取极性代谢组分,N,O-双(三甲基硅基)三氟乙酰胺衍生化处理,通过GC-MS分离鉴定代谢产物。结果表明,同一产地2?个不同品种小米共发现了53?种代谢产物。古龙小米有48?种代谢产物,其中优势代谢产物为亚油酸;禾绅小米代谢产物有32?种,优势代谢产物为亚油酸。对比发现,古龙小米有21?种差异代谢产物;禾绅小米有5?种差异代谢产物。对2?个品种小米差异代谢产物的代谢途径分析,发现古龙小米的葡萄糖代谢途径、脂肪酸代谢途径、氨基酸代谢途径和三羧酸循环都比禾绅小米对应的代谢途径更活跃,推测古龙小米的代谢产物多是因为其代谢速率快于禾绅小米的代谢速率。 相似文献
5.
目的研究食品包装材料中丙烯酰胺(acrylamide)的体外代谢情况,并确证其代谢产物。方法采用肝微粒体及肝S9体外温孵法,优化代谢条件,对丙烯酰胺进行了体外代谢研究,并用液相色谱-串联质谱法(liquid chromatography-tandem mass spectrometry,LC-MS/MS)检测并确证丙烯酰胺的代谢物。结果通过检测结果发现,丙烯酰胺分别在肝S9和肝微粒体作用下发生显著的代谢反应,其代谢产物之一为环氧丙酰胺。结论丙烯酰胺能通过代谢转化成毒性更强的环氧丙酰胺,因此,监控食品、饮水和周围环境中的丙烯酰胺的含量对于维护人们的健康具有重要的意义。 相似文献
6.
目的建立气相色谱-质谱法测定食品接触材料中润滑剂(油酸酰胺、硬脂酰胺、芥酸酰胺、山嵛酰胺)在脂类食品模拟物中迁移量的方法。方法采用异辛烷、95%乙醇、改性聚苯醚3种试验介质替代脂类食品试验,用三氟乙酸酐衍生后,采用气相色谱-质谱法测定。结果油酸酰胺、硬脂酰胺在0.2~20 mg/L范围内线性良好,芥酸酰胺、山嵛酰胺在1.0~30 mg/L范围内线性良好,方法定量限为0.026~0.14 mg/L,加标回收率为76.2%~97.3%,相对标准偏差(RSD)为1.2%~6.3%。结论本法简便、快速、准确,灵敏度及精密度较高,适用于食品接触材料中润滑剂在脂类食品模拟物中迁移量的测定。 相似文献
7.
采用水蒸气蒸馏法提取红提葡萄的挥发性成分,利用GC-MS技术进行分析,分离并确定了53个组分,其中3-羟基-2-丁酮、1.羟基-2.丙酮、糠醛、2,4-二羟基.2,5.二甲基-3(2H).呋喃酮)、丁内酯、糠醇、苯乙醇、糠酸甲酯、5-羟甲基糠醛、亚油酸乙酯、肉豆蔻酸、棕榈酸、棕榈烯酸、油酸、亚油酸和亚麻酸其相对含量分别为1.36%、1.79%、18.547%、7,04%、2,22%、1.07%、11.08%、3.524%、3.06%、1.11%、1,48%、12.21%、2.55%、4.11%、9.58%和4.34%。表明红提葡萄为一种非芳香型品种。 相似文献
8.
9.
测定食品中苯并[a]芘的气相色谱-质谱法研究 总被引:1,自引:0,他引:1
研究了用气相色谱—质谱法测定食品中的苯并[a]芘的含量,该方法与荧光分光光度法比较具有可操作性强、准确度高和避免实验人员长时间受紫外线的照射等特点,可以定量测定苯并[a]芘的含量。并对特性基本相同的各种样品进行了加标回收检验,其回收率在85%以上,相关系数〉0.9980,RSD〈6.5%。 相似文献
10.
为明确鲁氏酵母菌代谢合成呋喃酮工艺条件与代谢情况,以鲁氏酵母(Zygsoaccharomyces rouxii)QOR6为出发菌株,通过单因素实验和正交试验优化QOR6代谢合成呋喃酮的发酵条件,并采用气相色谱-质谱联用技术测定其挥发性香气物质的种类和相对含量。结果表明,当QOR6发酵温度28℃、发酵初始pH4.2、培养转速为180 r/min和接种量为20×107 CFU/mL时,呋喃酮的产量为2.90 mg/L,比优化前提高了1.09倍。发酵液中挥发性成分共有41种,主要包括醇类、醛类、酯类、芳香族和烃类等。其中,醇类含量最高,占总挥发物的81.13%。其他相对含量较高的为乙酸苯乙酯(3.48%)、甲醛(0.34%)、2,6-二叔丁基苯酚(8.77%)、氯甲氧基甲烷(0.20%)和3-乙基-2,5-甲基吡嗪(0.70%)。本文旨在为鲁氏酵母菌在食品中的应用提供了技术支持。 相似文献
11.
总迁移量作为食品接触材料的关键的卫生性能指标之一,是企业质量控制和政府监督管理中的重要参数。油脂模拟物中总迁移量反映了预期接触油脂类食品接触材料的质量安全。实验室目前多采用有机溶剂替代橄榄油进行测试,但是替代试验结果往往存在偏差,难以反应正确真实的迁移情况。本文比较了国内外油脂类总迁移量的检测方法,探究了橄榄油与有机溶剂总迁移量差异,为改进我国总迁移量检测方法提供一些依据和参考。 相似文献
12.
输欧食品接触材料风险分析 总被引:7,自引:1,他引:7
通过汇总和分析2000~2010年欧盟食品与饲料快速预警系统(RASFF)对原产自中国(含中国香港)的食品接触材料的通报数据,得出尼龙和蜜胺塑料餐厨具、不锈钢餐厨具为中国输欧食品接触材料中的高风险产品,芳香胺迁移量、甲醛迁移量和铬镍重金属迁移量为高风险检测项目,意大利、德国、英国等为敏感性高风险输入国,并分析通报原因。为企业有的放矢提高产品质量、规避贸易风险提供帮助,并建议监管部门及时向出口企业宣贯、讲解欧盟法规和技术标准要求,通报欧盟RASFF通报情况、高风险产品和项目,使企业提高产品质量控制意识,积极主动学习了解国外法规和通报情况。 相似文献
13.
《Contact lens & anterior eye》2021,44(5):101430
PurposeSeveral clinical studies have suggested that reusable silicone hydrogel contact lens materials exhibit a two-times increased rate of corneal infiltrative events compared to reusable hydrogels. One potential factor contributing to this complication relates to the differential uptake of tear film-based pro-inflammatory cytokines. The purpose of this study was to use an in vitro assay to investigate whether four pro-inflammatory cytokines differed in their uptake onto six contemporary contact lens materials.MethodsConventional hydrogel (etafilcon A, omafilcon A) and silicone hydrogel (balafilcon A, comfilcon A, senofilcon A, somofilcon A) contact lens materials were soaked in solutions containing pro-inflammatory cytokines IL-1β, IL-6, IL-8 and TNF-α. Samples of the soaking solutions were collected over various time points and analyzed using the Meso Scale Discovery system, which served as a measurement of cytokine uptake onto the contact lens materials.ResultsBoth conventional hydrogels (etafilcon A, omafilcon A) and two of the four silicone hydrogels tested (balafilcon A, comfilcon A), exhibited some uptake of IL-1β, IL-8 or TNF-α (p < 0.05). Senofilcon A and somofilcon A did not exhibit uptake of any of these cytokines (p > 0.05). There was no uptake of IL-6 onto any of the contact lens materials investigated (p > 0.05).ConclusionThe contact lens materials tested did not exhibit any uptake of IL-6 and furthermore, did not exhibit more than 10 ± 3 % to 25 ± 12 % uptake of IL-1β, IL-8 or TNF-α. Numerous factors could contribute to the reported increase in corneal infiltrative events with reusable silicone hydrogel materials, however, based on these results, it appears that uptake of these four cytokines are unlikely to contribute to this finding. 相似文献
14.
15.
《Contact lens & anterior eye》2022,45(4):101490
Smoking is a risk factor for the development of microbial keratitis and corneal infiltrates in contact lens (CL) wearers. It is still unknown if this risk is directly associated with the presence of nicotine in the eye and if adherence of nicotine on the CL can enhance these effects. A better understanding of the interaction between nicotine and CL materials could offer insights to explain this risk associated with smoking. The aim of this work was to compare the affinity of nicotine to different soft CL materials. CLs from FDA groups I, II, IV, and V were incubated in a 2-mM nicotine solution for 24 h and then in a 0.9% saline solution for the next 24 h. The amount of absorbed and released nicotine per CL was deduced as a function of time (t) by ultraviolet (UV) spectrophotometry and normalised to the mass of the hydrated CL. The data were described by the equation y = b –a t?1, where a and b are constants, and b represents the mass reached at the plateau after ~ 10 min of exposure. Groups IV and V displayed the highest (0.80 ± 0.09 µg) and lowest (0.27 ± 0.08 µg) nicotine absorption per mg of hydrated CL, respectively. The CL affinity for nicotine could be ascribed to the interaction between the positive charge of nicotine pyrrolidine nitrogen and the negative charges of the CLs, especially for the ionic IV group. 相似文献
16.
17.
目前食品接触材料安全性日益受到关注。食品接触材料产生的挥发性物质,常常具有异常的气味,可能通过气相传质或直接接触迁移至食品中,不但会引发食品感官特性的变化,影响消费者的使用体验,削弱消费者购买欲望,过量摄入后还会危及人体健康。因此,准确识别和检测食品接触材料产生的异味物质,对确保食品接触材料的合规以及保护消费者健康安全显得尤为重要。本文对国内外关于挥发性物质气味评估和检测技术及其在食品接触材料领域的运用进行综述。重点介绍了顶空、顶空-固相微萃取、热脱附、电子鼻等气味分析技术,及其与气相色谱、气相色谱/质谱或气相色谱-嗅闻-质谱仪等结合的联用分析技术,并梳理和展望了食品接触材料中挥发性气味物质分析技术发展前景,为有效管控食品接触材料异味问题提供技术参考。 相似文献
18.
《Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment》2013,30(7):1291-1300
Paper and board used as food contact materials (FCMs) are chemically complex matrices, partly due to the naturally occurring substances in paper and board, but also due to the chemical treatment of the paper used to make it suitable for food contact. In order to assure the safety of packaging materials, information on the exposure as well as on the toxicity of substances in the packaging must be obtained. This study describes a comprehensive method for the extraction and fractionation of substances present in paper and board FCMs for further investigation by in vitro testing and chemical analysis. The extraction efficiency and the fractionation process were validated by determining recoveries in extracts from paper and board fortified with five surrogates of known concentration. The recoveries for the five surrogates were between 20% and 104% in the raw extract and between 21% and 109% after extraction and fractionation. The fractionation both reduces the number of compounds to be identified and works as a sample clean-up by reducing matrix effects. Raw extracts and fractions from two paper and board FCMs were furthermore tested in the aryl hydrocarbon receptor (AhR) reporter gene assay. Both raw extracts and two of the fractions of the raw extracts gave a positive response in the AhR assay. The strategy of extraction followed by fractionation offers a powerful tool in order to make the workflow for screening FCMs for potentially adverse effects more efficient. 相似文献