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高分辨快原子轰击质谱法及其在生物医学分析中的应用 总被引:1,自引:1,他引:0
快原子轰击质谱经过十几年的发展,已成为分析极杜强、难挥发和热不稳定化合物囊常用的质谱方法,然而用快原子轰击质话进行精确质量测定的应用和研究开展还不很广泛.本文简要介绍了高分辨快原子轰击腐语法的方法和特点,并概述了高分辨快原子轰击质谱在生物医学分析几个主要方面(生物小分子、生物大分子、药物代谢产物和临床病理研究等)中的应用. 相似文献
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生物质谱在核糖核酸领域的应用 总被引:5,自引:1,他引:5
综述了近十年来生物质谱用于分析核糖核酸的应用进展。概述了电喷雾电离质谱(ESI-MS)、基质辅助激光解析电离质谱(MALDI-MS)的原理。论述了生物质谱用于分析生物大分子具有准确、快速、灵敏度高等优点。总结了质谱用于单核苷酸多态性分型分析(SNP genotyping);对短的串联重复序列(STR)分析;基因缺陷而导致的疾病诊断;对寡核苷酸片段的序列分析等4个方面的研究成果。讨论了应用ESI-MS分析寡核苷酸形成的非共价复合物,包括研究寡核苷酸的高级结构、寡核苷酸与蛋白质间形成的复合物、寡核苷酸与其它配体小分子间相互作用。提出了存在的问题,并展望了生物质谱未来的发展方向。 相似文献
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质谱技术在中药小分子与生物大分子相互作用研究中的应用 总被引:1,自引:0,他引:1
中药发挥药理作用具有多组分,多靶点的重要特点。研究中药化学成分与生物大分子之间的相互作用不仅能够为阐明中药发挥药理作用的机理和物质基础提供科学依据,而且能够为新药设计提供理论指导。软电离质谱技术,尤其是电喷雾质谱(ESI-MS)和基质辅助激光解吸电离质谱(MALDI-MS)在一定条件下能够使药物与生物大分子形成的复合物完整地转移到气相中并被检测到,在中药小分子与生物大分子相互作用的研究中具有很大的优势。同时,色谱-质谱联用技术在中药复杂体系与生物大分子相互作用的研究中也显示出很大的应用潜力。本文介绍了质谱技术在药物与生物大分子相互作用研究中的应用原理,并总结了近年来软电离质谱技术在中药小分子与生物大分子相互作用研究中的应用。 相似文献
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综述生物质谱用于分析核糖核酸的应用进展。概述电喷雾电离质谱(ESI-MS)和基质辅助激光解吸电离质谱(MALDI-MS)的原理及它们在核酸分析中的应用。总结质谱用于单核苷酸多态性分型分析(SNPgenotyping);对短的串联重复序列(STR)的分析;对寡核苷酸片断的序列分析等三个方面的研究成果。提出质谱用于基因组学研究存在的问题,并展望生物质谱未来的发展方向。 相似文献
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蛋白质组与生物质谱技术 总被引:11,自引:2,他引:9
蛋白质组是基因组研究的继续。蛋白质组的研究是为了识别及鉴定一个基因组、一个细胞或组织所表达的全部蛋白质以及它们的表达模式。以基质辅助激光解吸附飞行时间质谱和电喷雾质谱为代表的现代生物质谱技术,为蛋白质组的研究提供了必要的技术手段,被称为蛋白质组研究的三大关键性支撑技术之一。而质谱—质谱联用、质谱与其它技术联用以及高产出筛选技术的应用和发展,将使蛋白质组的研究在高准确度、高灵敏度以及大规模化水平上的发展成为可能。 相似文献
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László Tőkés 《Mass spectrometry reviews》2017,36(4):520-542
This reminiscing review article is an account of the author's fascination and involvements with mass spectrometry from the perspective of an organic chemist with an interest in natural product chemistry. It covers a period from 1961 through the mid 1990s as mass spectrometry evolved form a novelty technique to become a most widely used analytical technique. Following a brief synopsis of my pathway to mass spectrometry, my research efforts in this field are presented with a focus mainly on evolving principles and technologies which I had personal involvements with. To provide historical perspectives, discussions of these developments are accompanied by brief outlines of the relevant state‐of‐the‐art, shedding light on the technical and conceptual challenges encountered during those early days in mass spectrometry. Examples are presented of my involvements with basic and applied research in mass spectrometry during graduate studies at Stanford University and close to three decade tenure in pharmaceutical research at Syntex Research. My basic research interests focused mainly on principles of electron ionization induced fragmentation mechanisms, with an emphasis on steroids and other model compounds. Extensive deuterium labeling evidence was used to determine the fragmentation mechanisms of the diagnostically significant ions in the spectra of numerous model compounds, uncovering examples of wide‐ranging hydrogen transfers, skeletal rearrangements, methyl and phenyl migrations, stereoselective fragmentations and low and high energy fragmentation processes. Depiction of the industrial research phase of my career includes comments on the pivotal role mass spectrometry played on advancing modern pharmaceutical research. Examples are presented of involvements with instrumental developments and a few select cases of applied research, including studies of bile mechanisms in vertebrates, identification of bisphenol‐A leaching from sterilized polycarbonate containers, high sensitivity TCDD analyses and other projects. Reflecting on my services for the mass spectrometry society, involvements with the co‐founding and 12 year chairing of the Asilomar Conference on Mass Spectrometry and founding of the Bay Area Mass Spectrometry regional MS discussion group, as part of my services for the mass spectrometry community, are presented in some detail. © 2016 Wiley Periodicals, Inc. Mass Spec Rev 36:520–542, 2017 相似文献
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岛津公司有机质谱新技术及应用进展 总被引:1,自引:0,他引:1
本文概述了近年岛津公司在质谱技术上取得的新进展和相关应用,包括:GCMS的新技术特点,GCMS衍生仪器技术如GPC-GCMS、MDGC/GCMS和全二维气相色谱质谱联用技术及其应用;全新LCMS-8030三重四极杆质谱仪的技术特点和在应对超快速分析中的特长;LCMS-IT-TOF在复杂基质中多种化合物的高质量精度和多级质谱分析技术上独特的优势;Axima-QITTM技术特点及在蛋白质组学分析中的技术优势;解吸电晕束直接电离源DCBI技术和相关应用等。 相似文献
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Characterization and differentiation of isomers in biological macromolecules using mass spectrometry is one of the most significant challenges facing scientists in the field. The capability of high‐resolution MS instruments along with the development of new fragmentation methods now provides the ability to indirectly differentiate between some isomers. This ability has enabled mass spectrometry to evolve into a multidisciplinary technique incorporating areas such as pharmaceutical research, proteomics, polymer science, medicine, environmental chemistry, and recently archeology. This article aims to review recent developments in mass spectrometry methodologies in the identification of structural and spatial isomers in biological macromolecules, such as aspartic acid and isoaspartic acid (Asp/IsoAsp), leucine and isoleucine (Leu/Ile), glutamic acid and γ‐glutamic acid, and D/L enantiomers. © 2012 Wiley Periodicals, Inc. Mass Spec Rev 31:609–625, 2012 相似文献
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2—(对二茂铁基苯基)—1,3—二噻烷衍生物的质谱研究 总被引:2,自引:1,他引:2
本文研究了2-(对二茂铁基苯基)-1,3-二噻烷衍生物的EI谱特征。并采用B/E联动扫描研究了这类化合物质谱裂解的规律。 相似文献
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应用基质辅助激光解吸电离飞行时间质谱法(MALD I-TOF-MS)成功地对核糖核酸酶相对分子量进行测定。探讨三种不同基质对其分析结果的影响,实验中发现α-氰基-4-羟基肉桂酸是适宜的基质,该方法是传统生物方法无法比拟的。 相似文献