甘氨酸螯合铁纳米脂质体对缺铁性贫血大鼠的补铁效果

研究了甘氨酸螯合铁纳米脂质体作为补铁剂对缺铁性贫血大鼠的补铁效果。通过对SD品系初断乳大鼠喂养缺铁基础饲料,建立缺铁性贫血大鼠模型,再用甘氨酸螯合铁纳米脂质体对缺铁性贫血大鼠补铁,并与甘氨酸螯合铁和硫酸亚铁进行比较。结果表明,甘氨酸螯合铁纳米脂质体能够显著增加大鼠血液中血红蛋白含量,提高血清铁含量,降低血清总铁结合力(P<0.05);并且它能够有效地促进大鼠肝脾组织中铁含量的增加。因而,甘氨酸螯合铁纳米脂质体可显著改善大鼠的缺铁性贫血状况,且比甘氨酸螯合铁和硫酸亚铁有更好的补铁效果。     

甘氨酸螯合铁对大鼠补铁效果的评价

分别比较了甘氨酸螯合铁和硫酸亚铁对健康大鼠和缺铁贫血大鼠的补铁效果.对于健康大鼠,甘氨酸螯合铁较硫酸亚铁有更好的吸收利用效果.双倍剂量的甘氨酸螯合铁组血清总铁结合力(TIBC)显著低于双倍剂量硫酸亚铁组(P＜0.01),甘氨酸螯合铁对肝脏指数和肝脏铁质量分数的提高都有显著的促进作用.对于缺铁贫血大鼠,甘氨酸螯合铁补铁比硫酸亚铁见效快.补铁4 d,甘氨酸螯合铁组的血清铁质量分数、肝脏指数和肝脏铁平均质量分数均比硫酸亚铁组显著提高(P＜0.05);补铁7 d,两种铁源血清铁水平没有显著差异,但甘氨酸螯合铁组血清总铁结合力、肝脏指数和肝脏铁质量分数显著高于硫酸亚铁组(P＜0.05).     

氧化还原状态对肉仔鸡铁代谢利用的影响

对肉仔鸡给予含硫酸亚铁的补铁日粮,并在补铁日粮中添加不同水平的半胱胺。研究氧化还原状态对肉仔鸡体内铁代谢利用的影响。结果表明:试验组血红蛋白、血浆铁、肝脏铁含量升高,血浆总铁结合力降低。试验组的血浆丙二醛含量较对照组降低,血浆谷胱甘肽过氧化物酶活力、谷胱甘肽含量和总抗氧化能力显著升高(P<0.05)。这说明,半胱胺能够提高肉仔鸡机体的抗氧化性能,改变机体的氧化还原状态,促进肉仔鸡体内铁的代谢和利用,以80 mg/kg的添加水平最为显著。     

谷氨酸钠对缺铁大鼠铁代谢的影响

试验选用大鼠作为模型,研究谷氨酸钠对大鼠的铁代谢指标的影响。试验大鼠30只分为3个试验组:对照组、FeSO_4组和FeSO_4-谷氨酸钠试验组(日粮中添加1%味精)。试验结果显示:1)日粮中添加味精的缺铁大鼠补铁后,大鼠体重有所增加(P0.05)。2)日粮中添加味精的缺铁大鼠灌胃补铁后,贫血大鼠血清铁和铁蛋白均恢复到正常水平,和对照组相比显著增高(P0.05);和FeSO_4相比,FeSO_4-味精组血红蛋白含量增加了3.97%(P0.05)。结果显示味精不但不影响动物对铁的吸收,还有一定的促进铁吸收作用。     

鸡蛋卵转铁蛋白螯合铁及其对大鼠贫血模型的影响

将从鸡蛋中分离纯化的卵转铁蛋白与FeCl3结合,研究其在不同铁离子浓度、pH值、NaHCO3和温度条件下与铁离子的结合能力。通过色度仪、红外光谱仪、粒径分析仪、扫描电镜等仪器研究冷冻和喷雾干燥对螯合铁卵转铁蛋白理化性质的影响。在0.4 mmol/L FeCl3,120 mmol/L NaHCO3,pH 8.0,温度低于60℃时,卵转铁蛋白铁结合能力为91.5%,结合的铁含量为1.28μg/g。冷冻和喷雾干燥对螯合铁卵转铁蛋白的色度、粒径分布、完全溶解时间存在极显著的差异,而在水分活度和化学键结构方面无明显差异。建立缺铁大鼠模型,考察30 d内卵转铁蛋白对大鼠贫血的改善作用,结果表明:冷冻和喷雾干燥处理的螯合铁卵转铁蛋白对贫血大鼠体重和脏器指数升高有显著作用;对血常规中的红细胞计数、血红蛋白浓度、血细胞压积、红细胞平均体积均有正向促进作用(P<0.01),能够显著增加大鼠血液中血清铁含量,提高血清铁蛋白含量,降低血清总铁结合力(P<0.05),部分指标优于对照组硫酸亚铁。卵转铁蛋白可作为一种生物补铁剂显著改善大鼠的缺铁性贫血状况。     

缺铁性贫血大鼠模型建立和补充乳酸亚铁改善血红蛋白水平研究

为探讨缺铁性贫血大鼠模型建立及补充乳酸亚铁后大鼠生长发育和血红蛋白水平的变化，为相关研究提供参考数据，采用二级初断乳Wistar大鼠25只，体重50—60g，在二级动物房单笼饲低铁饲料4周，测定尾血血红蛋白（Hb），将Hb低于100g／L的大鼠依血红蛋白水平随机分成缺铁对照组和补充铁组，每组10只。实验期为4周。缺铁大鼠和乳酸亚铁补充组大鼠的及其差值均有差异有非常显著性（P〈0．0001）；缺铁性贫血大鼠肝脏重量和心脏重量低于补充乳酸亚铁组大鼠（P〈0，0002），而脾脏和肾脏重量高于补充乳酸亚铁组大鼠，两组脾脏重量差异有显著性（P〈0．05）；缺铁性贫血大鼠的脾脏，体重与心脏，体重显著大于补充乳酸亚铁组大鼠（P〈0．05），补充乳酸亚铁组大鼠的肾脏，体重则大于缺铁性贫血大鼠（P=0．001）；缺铁对照组大鼠的血红蛋白含量非常显著低于补充乳酸亚铁组大鼠（P〈0，0001），而缺铁对照组大鼠的红细胞游离原卟啉含量非常显著高于补充乳酸亚铁组大鼠（P〈0．0001），缺铁组大鼠的白细胞数量是补充组的3．4倍，红细胞数量却仅为补充组的33．9％。缺铁大鼠模型建立需要严格控制饲养条件，缺铁大鼠通过一定的代偿性增生改善机体适应缺铁和缺氧状况的能力有限，补充乳酸亚铁后可以有效地改善缺铁状况。     

鱿鱼墨黑色素铁对缺铁性贫血大鼠的影响

为了研究鱿鱼墨黑色素铁对缺铁性贫血大鼠的影响。采用56只断乳SD大鼠,随即选取8只饲喂正常饲料,另外48只饲喂低铁饲料28 d,造成缺铁性贫血模型后随即分为6组。其中3组分别灌胃不同剂量的鱿鱼墨黑色素铁(分别为6,12,18 mg/kg)21 d,阳性组分别灌胃硫酸亚铁和氯化铁(6 mg/kg),缺铁模型组和对照组分别每天灌胃等体积的去离子水共21 d。测定各组大鼠血液指标、脏器指数、血清铁、肝脏和脾脏的铁含量、抗氧化性能指标、血红细胞生成素、转铁蛋白受体和铁蛋白含量。结果表明,鱿鱼墨黑色素铁各剂量组和阳性组均可提高大鼠血红蛋白含量,提高血清、肝脏和脾脏铁含量、提高血清铁蛋白和促红细胞生成素含量,提高血清和肝脏抗氧化能力,降低血清总铁结合力和转铁蛋白受体含量,改善缺铁性贫血,比FeSO4和FeCl3的效果更佳。因此,鱿鱼墨黑色素铁可作为潜在的有效的补铁剂。     

鱿鱼墨黑色素铁对缺铁性贫血大鼠的影响

为了研究鱿鱼墨黑色素铁对缺铁性贫血大鼠的影响。采用56只断乳SD大鼠,随即选取8只饲喂正常饲料,另外48只饲喂低铁饲料28 d,造成缺铁性贫血模型后随即分为6组。其中3组分别灌胃不同剂量的鱿鱼墨黑色素铁(分别为6,12,18 mg/kg)21 d,阳性组分别灌胃硫酸亚铁和氯化铁(6 mg/kg),缺铁模型组和对照组分别每天灌胃等体积的去离子水共21 d。测定各组大鼠血液指标、脏器指数、血清铁、肝脏和脾脏的铁含量、抗氧化性能指标、血红细胞生成素、转铁蛋白受体和铁蛋白含量。结果表明,鱿鱼墨黑色素铁各剂量组和阳性组均可提高大鼠血红蛋白含量,提高血清、肝脏和脾脏铁含量、提高血清铁蛋白和促红细胞生成素含量,提高血清和肝脏抗氧化能力,降低血清总铁结合力和转铁蛋白受体含量,改善缺铁性贫血,比FeSO4和FeCl3的效果更佳。因此,鱿鱼墨黑色素铁可作为潜在的有效的补铁剂。     

血红蛋白多肽螯合铁的抗贫血功能研究

以Wiser品系初断乳大鼠为缺铁贫血模型,研究了血红蛋白多肽螯合铁对改善大鼠缺铁性贫血的效果,并与葡萄糖酸亚铁和氟化亚铁进行了对比.研究结果表明,血红蛋白多肽螯合铁可使贫血大鼠的体重、血红蛋白、红细胞计数、血清铁水平显著升高(P<0.05),说明血红蛋白多肽螯合铁可显著改善大鼠的生长以及缺铁性贫血,并且其补铁效果明显好于葡萄糖酸亚铁和氯化亚铁.     

乳猪小肽螯合铁的补铁效果

采用小肽螯合铁作为补铁剂,妊娠和泌乳母猪以及乳猪为研究对象,测定母猪初乳铁的含量,乳猪5、10、14和21日龄的血红蛋白（Hb）以及乳猪14和21日龄的血清铁蛋白（SF）浓度.结果表明,小肽螯合铁比硫酸亚铁能够更有效地进入母乳,母猪补小肽铁可增加乳猪铁的获得,但不能解决乳猪的缺铁问题;乳猪补小肽螯合铁不但有较高的Hb水平,而且体内铁储备量高于补右旋糖苷铁,可满足乳猪铁的需要.     

苏氨酸铁对缺铁性贫血大鼠铁营养状况的改善

目的:探讨苏氨酸铁(Thr-Fe)对缺铁性贫血(IDA)大鼠铁营养状况的改善作用。方法:以低铁饲料饲喂雄性初断乳斯泼累格·多雷(SD)大鼠建立IDA模型,设置3个不同Thr-Fe剂量组和阳性对照组、低铁对照组、空白对照组,每组6只,每天灌胃1次,连续给药21 d,Thr-Fe剂量组分别给予8、16、32 mg/kg·bw的Thr-Fe,阳性对照组给予32 mg/kg·bw硫酸亚铁。结果:较低铁对照组,Thr-Fe高剂量组大鼠体重及血红蛋白、血清铁蛋白等血液指标显著提高(p<0.01),血清转铁蛋白(p<0.01)和总铁结合力(p<0.05)显著降低,各Thr-Fe剂量组大鼠游离原卟啉显著降低(p<0.01)。Thr-Fe高剂量组IDA大鼠各项指标改善优于阳性对照组。结论:低铁膳食补充Thr-Fe可以改善IDA大鼠的铁营养状况,提高功能铁及贮存铁含量,促进机体铁转运。Thr-Fe对IDA大鼠体重及血红蛋白等的影响呈剂量相关性,Thr-Fe对IDA大鼠铁营养状况的改善效果优于硫酸亚铁。     

Iron absorption and haemoglobin status of rats fed a ferrous bisglycinate‐fortified growing‐up milk

BACKGROUND: Infants and toddlers aged 6–24 months constitute one of the groups at highest risk of iron deficiency. A promising alternative for ferrous sulfate (FS) might be ferrous bisglycinate (FeAAC), which is less likely to cause sensory changes in the food vehicle. This work aims to compare the effect of FeAAC with that of FS, when added to a growing‐up milk, on the iron and haemoglobin status in weanling rats using a depletion–repletion model. RESULTS: After the repletion period no significant differences were found in iron absorption, Hb concentration, Hb iron, Hb regeneration efficiency (HRE), mean corpuscular volume (MCV), mean corpuscular Hb (MCH), mean corpuscular Hb concentration (MCHC), serum iron, total iron binding capacity (TIBC), transferrin saturation (TS), and ferritin between the group fed a growing‐up milk fortified with FS and the group fed one fortified with FeAAC. Furthermore, iron absorption, HRE, MCV, MCH, serum iron, and TS were significantly (P < 0.05) higher in groups fed either type of iron‐fortified growing‐up milk compared to the control group (AIN‐93G diet). CONCLUSION: A growing‐up milk supplemented with FeAAC showed an improvement in the iron absorption and haemoglobin status at the same level as those of FS. Copyright © 2009 Society of Chemical Industry     

Bioavailability of iron in goat milk compared with cow milk fed to anemic rats

Bioavailabilities of iron from dehydrated whole and skim goat milk were investigated using iron-deficient rats. Hemoglobin regeneration efficiencies were determined as the percent conversion of dietary iron into hemoglobin. The respective hemoglobin regeneration efficiencies for groups fed whole goat milk, whole cow milk, skim goat milk, and skim cow milk were 50.6, 13.1, 26.0, and 13.0%, indicating that iron bioavailability of goat milk was greater than cow milk. However, rats fed each milk had negative net increases in hemoglobin concentrations, implying that the iron contents of each milk were not adequate. For animals consuming whole goat milk supplemented with ferrous sulfate, the slope relating hemoglobin iron gained versus iron intake was .95. Respective bioavailabilities relative to ferrous sulfate were 54, 14, 28, and 14% for the four sources of milk. Iron bioavailability of goat milk is superior to cow milk when fed to anemic rats.     

Influence of high dietary iron as ferrous carbonate and ferrous sulfate on iron metabolism in young calves

Twelve intact male Holstein calves averaging 90 kg and 12 wk of age were fed one of three dietary treatments for 28 d. The diets were A) control, B) control plus 1000 ppm iron as ferrous carbonate, and C) control plus 1000 ppm iron as ferrous sulfate monohydrate. Calves were dosed orally on d 15 of the treatment period with 1 mCi of iron-59. Neither source of added iron had a significant effect on weight gains, feed consumption, hemoglobin, packed cell volume, serum total iron, serum total iron-binding capacity, unbound iron-binding capacity, serum copper, tissue copper, fecal dry matter, or a consistent effect on fecal pH. The ferrous carbonate had no significant effect on stable zinc or stable iron in any tissue studied. Calves fed ferrous sulfate had higher average stable iron in most tissues and significantly more in the small intestine. Tissue zinc was lower in spleen and pancreas of ferrous sulfate-fed calves. Both sources of added iron sharply reduced iron-59 in serum, whole blood, and body tissues. The reduction was substantially greater in calves fed the ferrous sulfate iron. Iron in ferrous sulfate had a higher biological availability than that in the ferrous carbonate; however, bioavailability of the ferrous carbonate iron appeared to be substantial and considerably more than that noted in previous studies in which a different source of ferrous carbonate was used. The maximum safe level of dietary iron is materially influenced by the source of iron with a higher tolerance indicated for ferrous carbonated than ferrous sulfate monohydrate.     

Bioavailability to rats of iron from fortified grain amaranth flour

In this study, fortified and unfortified grain amaranth seed flour and FeSO₄ fortified casein diet as control were evaluated for their iron bioavailability. NaFeEDTA, ferrous fumarate, and FeSO₄-fortified grain amaranth were fed to Sprague Dawley weanling male rats. Iron intake, Hb Fe gain, Fe availability and binding capacity, serum iron, non-haem liver iron and relative biological value (RBV) were determined and values compared with FeSO₄-fortified casein control; all were improved greatly by fortification.
Relative biological values (RBV) were 0.40, 1.55, 1.75, 1.67 and 1.00 for animals receiving unfortified amaranth diet, those fed diet fortified with NaFeEDTA, ferrous fumarate, FeSO₄ and casein fortified with FeSO₄ respectively. RBV of the unfortified cereal was 40% of control suggesting perhaps low iron absorption from the amaranth cereal.
Based on the results of this study, amaranth cereal is ideal as a food vehicle for iron fortification. The iron fortificant of choice is ferrous fumarate.     

Relative Bioavailability of Dietary Iron from Three Processed Soy Products

The relative bioavailability of iron from soy flour (SF), freeze-dried soy beverage (SB) and soy concentrate (SC) was determined utilizing a hemoglobin repletion bioassay. Weanling male rats were fed a low iron depletion diet (3.5 ppm Fe) for 4 wk. For the next 2 wk groups of rats were fed repletion diets containing 0, 6, 12, or 18 ppm added iron from ferrous sulfate, SF, SB, or SC. Slope ratio analysis revealed that the relative iron bioavailabilities from SC (92%) and SF (81%) were not different from the reference standard, ferrous sulfate added to a casein-based diet, whereas that from SB (66%) was significantly less (P<0.01) than the inorganic source of iron. Analysis of results at individual iron levels suggested an iron bioavailability of SC>SF>SB.     

Effect of Heat Treatment on Bioavailability of Meat and Hemoglobin Iron Fed to Anemic Rats

Efficiency of converting dietary iron from meat, bovine hemoglobin (HB) and ferrous sulfate into hemoglobin was investigated in anemic rats. Raw or autoclaved HB, and raw, autoclaved, boiled, or baked beef round, and ferrous sulfate were mixed into diets to give 36 mg Fe/kg diet. Heat treatments increased the efficiency of converting both HB and meat iron into hemoglobin by the anemic rats. Efficiencies of conversion were 23, 30, 33, 37, 37, 36 or 60 (LSD 0.05/0.01 = 4/6), respectively, for raw HB, autoclaved HB, raw meat, autoclaved meat, boiled meat, baked meat or ferrous sulfate. An in vitro measurement of iron availability in meat correlated poorly with bioavailability determined in rats. Cooking did not significantly affect the bioavailability of meat iron.     

香菇、黑木耳提取液改善大鼠营养性贫血的 效果评价

目的 考察香菇、黑木耳提取液改善营养性贫血的效果。方法 将SD大鼠以缺铁饲料造成营养性贫血模型, 把大鼠随机分为低铁模型组、香菇提取液灌胃组、黑木耳提取液灌胃组、乳酸亚铁对照组及正常对照组, 比较分析了各组大鼠的体重、摄食量、血红蛋白(Hb)含量、红细胞游离原卟啉(FEP)含量、红细胞压积的变化。结果 实验4周后, 香菇、黑木耳提取液灌胃组大鼠与低铁模型组比较, 体重、摄食量、血红蛋白含量与红细胞压积均显著升高(P<0.05), FEP含量则显著降低(P<0.01); 与乳酸亚铁组比较, 中、高剂量组大鼠血红蛋白含量、红细胞压积明显提高(P<0.05), FEP含量显著降低(P<0.01), 体重、摄食量略有升高; 各提取液灌胃组各项指标均接近正常组。结论 香菇、黑木耳提取液均可明显改善营养性贫血大鼠症状。