玫瑰果营养成分分析

对从荷兰引种的玫瑰果的营养成分进行分析并对红(R.rugosa Rubra)、白(R.rugosa Alba)2个品种玫瑰果的营养成分进行比较。结果表明,红玫瑰果的营养价值高于白玫瑰果,其中,红玫瑰果肉中VC的含量高达711.9 mg/100 g;矿物元素含量丰富,特别是含有营养价值较高的硒;黄酮类物质和β-胡萝卜素含量也较高,分别为690.5 mg/100 g和19.86 mg/100 g。果籽中脂肪酸以不饱和脂肪酸为主,其中亚油酸和亚麻酸含量高达50.10%和29.77%。因此,玫瑰果具有较高的营养价值和保健作用,具有较大的开发潜力。     

藿香玫瑰复合饮料研制及抗运动疲劳作用研究

采用藿香、玫瑰为原料,研制藿香玫瑰复合饮料。以感官得分作为评价指标,通过单因素与响应面优化试验确定藿香玫瑰复合饮料的最佳配方,分析其品质,并通过动物实验考察其抗运动疲劳活性。结果表明,藿香玫瑰复合饮料的最佳配方为:玫瑰与藿香提取液的体积比2.6:1、蔗糖用量4%、柠檬酸用量0.7%、羧甲基纤维素钠用量0.4%、黄原胶用量0.05%,感官得分92.2分,所得饮料呈红褐色,具有藿香与玫瑰的混合香味、酸甜适中、风味独特,且无明显沉淀物,多糖与黄酮含量分别为0.95%±0.01%、0.76%±0.02%。不同剂量的藿香玫瑰复合饮料可延长小鼠的爬杆时间,提高其肝、肌糖原含量,同时显著增强乳酸脱氢酶活力,降低运动后体内的乳酸含量。因此,藿香玫瑰复合饮料具有较好的抗运动疲劳活性。     

玫瑰花最佳浸提工艺优化及其茶饮料的研制

为充分提取玫瑰花的营养成分,对其浸提工艺进行研究。根据Box-Benhnken中心组合试验设计原理,对料液比、提取温度和提取时间3因素优化组合,确定玫瑰花最佳浸提工艺参数:料液比1:61、提取温度89℃、提取时间62min,在此工艺条件下浸出量379.49mg/g。40%浸提液,添加5.5%白砂糖、0.01%柠檬酸,可制得有玫瑰花风味、营养丰富的茶饮料。     

藿香挥发性成分的GC-MS分析

采用同时蒸馏萃取法提取,用气相- 色谱质谱联用法结合计算机谱图检索,对藿香挥发性化学成分进行分析鉴定。结果表明：藿香挥发油得率为0.37%,共鉴定出79 种化合物,占分离物质的98.04%;其主要成分为胡椒酚甲醚(47.60%)、D- 柠檬烯(5.91%)、丁香烯(6.59%)、丁香酚甲醚(1.87%)、β- 衣兰油烯(1.42%)。     

煎饼自然发酵糊中酵母菌的分离鉴定

从煎饼自然发酵糊中,分离纯化出5个优势菌株,通过菌落和菌体形态观察、菌丝观察、子囊孢子观察、掷孢子形成试验、糖发酵试验、碳源和氮源同化试验、其他生理生化试验等对分离酵母菌进行鉴定,这5个菌株分别为酿酒酵母(Saccharom yces cerevisiae)、褶皱假丝酵母(Candidarugosa)、热带假丝酵母(Candidatropicalis)、发酵毕赤氏酵母(Pichiafermentans)、近平滑假丝酵母(Candidaparapsilosis)。以耐酸能力和糖化能力为指标,最终优选出酿酒酵母、褶皱假丝酵母、热带假丝酵母、发酵毕赤氏酵母为发酵煎饼适合的发酵菌株。     

野生藿香中多糖的提取与测定及抗氧化活性研究

对野生藿香中的多糖进行提取,利用苯酚硫酸法测定藿香中多糖的含量,设计正交实验探讨藿香多糖提取的最佳条件,对野生藿香粉末在Soxhelt提取器里用有机溶剂回流提取之后,再用质量分数为1.5%的碳酸钠溶液作为介质,在85℃时浸提30min,可使藿香中多糖的提取率达到极大值。利用纸色谱法分析藿香多糖的单糖组成,结果表明,藿香多糖中的单糖主要是葡萄糖和半乳糖,有少量的果糖和甘露糖。藿香多糖具有抗氧化活性,对Fenton反应产生的.OH具有明显地清除作用。     

Establishing DNA markers to differentiate Agastache rugosa and Pogostemon cablin,which are confusedly used as medicinal herbs,using real-time PCR

Food Science and Biotechnology - Agastache rugosa and Pogostemon cablin are used as medicinal herbs and aromatic plants and belong to the family Lamiaceae. Despite differences in composition and...     

Synthesis of hexyl butyrate (apple and citrus aroma) by Candida rugosa lipase immobilized on Diaion HP-20 using the Box-Behnken design

Food Science and Biotechnology - This study aims at the synthesis of hexyl butyrate by Candida rugosa lipase (CRL) immobilized on Diaion HP 20. The lipase load used was...     

响应面法优化玫瑰花色苷超声辅助提取工艺

采用超声辅助法提取玫瑰花色苷,通过单因素实验和响应面分析,优化玫瑰花色苷的提取工艺。以花色苷得率作为评价指标,在单因素实验的基础上,选取乙醇体积分数、提取温度、pH和提取时间四个影响因素进行响应面优化。结果表明,最优提取工艺为:乙醇体积分数55%、提取温度55℃、pH3.0、提取时间80 min,此条件下玫瑰花色苷得率为(10.07±0.098)mg/g,与预测值的相对误差为0.28%,说明响应面分析结果可靠。该法比对照组的提取工艺用时短、效率高。     

Lipase-Assisted Concentration of n-3 Polyunsaturated Fatty Acids from Viscera of Farmed Atlantic Salmon (Salmo salar L.)

ABSTRACT: In fish processing, viscera are generally considered waste products and often discarded. Our research objective was to use Atlantic salmon ( Salmo salar L.) viscera as a source of fish oil and to increase its concentration of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) by lipase-assisted hydrolysis. Lipids from fillets and viscera had similar fatty acid compositions. After the enzymatic hydrolysis, acylglycerols were isolated from free fatty acids, therefore increasing the concentrations of EPA and DHA in the acylglycerols. Among the 6 commercial lipases investigated, lipases from Pseudomonas cepacia and Candida rugosa were the most effective following incubation of viscera oil at 35 °C for 20 h. Salmon viscera were a good source for fish oil, and the concentration of DHA and EPA was doubled by using microbial lipases.     

东北藿香为原料的炖鱼用复合调味料的研制

以食药兼用的草本植物藿香为原料,经乙醇回流萃取后,与蒜、姜、大料等调味料进行科学组方复配,研制出集健康、美味、自然、方便快捷于一身,具有地方特色的炖鱼用复合调味料。     

RECOVERY OF PALM CAROTENE FROM PALM OIL AND HYDROLYZED PALM OIL BY ADSORPTION COLUMN CHROMATOGRAPHY

Crude palm oil and crude palm olein were hydrolyzed with lipase from Candida rugosa to produce a free fatty acid (FFA) rich oil. The percentages of FFA produced and carotene degradation after the hydrolysis process were determined. The palm oil and hydrolyzed palm oil were subsequently subjected to column chromatography. Diaion HP-20 adsorbent was used for reverse phase column chromatography at 50C. Isopropanol or ethanol, and n-hexane were used as the first and second eluting solvents, respectively. The objective of hydrolyzing the palm oil was to produce more polar FFA-rich oil in order to enhance the nonpolar carotene bind to the nonpolar HP-20 adsorbent in the column chromatography process. Hydrolyzing palm oil with lipase from Candida rugosa gave 30- and 60-fold, respectively, of FFA in the crude palm oil and crude palm olein in 24 h at 50C. Approximately, 15.56 and 17.48% of carotene degraded in crude palm oil and crude palm olein, respectively. For column chromatography, using isopropanol or ethanol as the first eluting solvent, unhydrolyzed oil and hydrolyzed oil showed the carotene recovery infraction two (carotene-rich fraction) of about 36–37 and 90–96%, respectively. Over 90% of carotene recovery was obtained from     

Effect of methanol and water contents on production of biodiesel fuel from plant oil catalyzed by various lipases in a solvent-free system

Methyl esters synthesized from plant oil and methanol by the methanolysis reaction are potentially important as a biodiesel fuel. The methanolysis of soybean oil by lipases from various microorganisms was investigated. Several of the lipases were found to catalyze methanolysis in a water-containing system without an organic solvent. The lipases from Candida rugosa, Pseudomonas cepacia, and Pseudomonas fluorescens displayed particularly high catalytic ability. The reaction rates of methanolysis catalyzed by the C. rugosa and P. fluorescens lipases decreased significantly when the water content was low, showing that water prevents the inactivation of these lipases by methanol. On the other hand, the methanolysis reaction rate catalyzed by the P. cepacia lipase remained high even under a low water content. In addition, the P. cepacia lipase gave high methyl ester contents in the reaction mixture up to 2 or 3 molar equivalents of methanol to oil, which is attributed to the P. cepacia lipase having substantial methanol resistance. For the same methanol content, the reaction rates of methanolysis catalyzed by the P. cepacia lipase increased with decreasing water content, and hence lipases strongly resistant to high methanol, such as that from P. cepacia, are desirable for use in methanolysis reaction processes.     

玫瑰花提取物与几种抗氧化物质之间的相互作用

目的:为玫瑰花的综合开发应用提供理论基础。方法:以玫瑰花提取物、没食子酸、儿茶素、槲皮素、VC、芦丁和BHT为材料,研究了它们之间混合后对DPPH·清除活性的增效或减效作用。结果:玫瑰花提取物和没食子酸、槲皮素、VC在低浓度下起到了增效作用,而其余材料或高浓度下,玫瑰花和不同抗氧化剂表现为减效作用。结论:低浓度的某些抗氧化物质与玫瑰花粗提物的混合其混合物溶液的抗氧化活性增强。     

Enantioselective esterification of racemic ibuprofen in isooctane by immobilized lipase on cellulose acetate-titanium iso-propoxide gel fiber

Lipase (Candida rugosa) was entrap-immobilized on cellulose acetate-titanium iso-propoxide gel fiber by the sol-gel method. The immobilized lipase was used for the direct synthesis of (S)-ibuprofen ester from racemic ibuprofen using propyl alcohol as an acyl acceptor in isooctane. The activity of the immobilized lipase was decreased to about 10-20% that of native lipase. However, the reaction was more enantioselective compared to that with native lipase. The stability for repeated use was improved by immobilization.     

对GB 5413.10-2010中维生素K_1检测的改进研究

通过对不同种酶进行研究对比之后发现,改用胰脂肪酶能够很好的使脂肪降解,避免了对维生素K1检测的干扰,而且其加标回收率和方法重现性都能满足GB/T 27404-2008的要求。     

基于磁性固定化酶的α-亚麻酸植物甾醇酯合成工艺研究

采用固载有Candida rugosa的磁性固定化酶为生物催化剂,并将其用于α-亚麻酸植物甾醇酯的酶促合成。通过响应面分析优化实验条件,得到最佳工艺参数为：固定化酶用量为25.2mg/mL,甾醇底物浓度为146.5μmol/mL,异辛烷为溶剂,55℃反应5h,得到的植物甾醇的酯化率为92.61%。该固定化脂肪酶经过简单的溶剂清洗可以重复使用10次,仍能保持71.8%的催化活性。     

软枣猕猴桃玫瑰醋发酵条件响应面法优化

该试验以软枣猕猴桃、玫瑰为原料制作果醋,在单因素试验的基础上,利用响应面法对软枣猕猴桃玫瑰果醋的发酵工艺条件进行优化。采用Design-Expert 8.0.6设计软件进行分析,分析结果显示最优发酵条件为：温度32 ℃,初始pH值3.4,初始酒精度为7%vol。在此条件下果醋酸度最高可达5.39 g/100 mL。三个因素与果醋酸度之间建立的回归模型均为极显著,可用于实际生产预测。     

发酵型苦水玫瑰花酱的制作工艺参数优化

以苦水玫瑰为原料加工发酵型玫瑰花酱,在单因素试验的基础上以酸花比、糖花比、发酵时间、发酵温度为试验因素,对发酵工艺进行响应面设计,得到最佳的工艺参数为糖花比1.2∶1,酸花比0.011∶1,发酵时间29 d,发酵温度30℃,此条件下制得的玫瑰花酱总糖5.23%,总酸0.49%,氨基酸0.15%,单宁1.12%,乙醇5.62%vol。具有典型的玫瑰色泽及玫瑰花和发酵香。     

4种新疆地产芳香植物精油抗氧化作用比较研究

采用体外研究方法比较新疆地产玫瑰、迷迭香、椒样薄荷和罗马甘菊4 种芳香植物精油的抗氧化活性。测定4 种精油对1,1- 二苯基苦基苯肼(DPPH)自由基、羟自由基和超氧阴离子自由基的清除能力,同时用硫代巴比妥酸(TBA)法测定4 种挥发油对脂质过氧化反应的抑制作用。结果表明：4 种精油均可清除DPPH 自由基、羟自由基和超氧阴离子自由基,并可抑制卵黄不饱和脂肪酸过氧化,且清除各类自由基及抑制脂质过氧化作用与样品挥发油质量浓度正向相关。4 种精油抗氧化活性顺序为玫瑰＞迷迭香＞椒样薄荷＞罗马甘菊。