全文获取类型
收费全文 | 3983篇 |
免费 | 928篇 |
国内免费 | 67篇 |
专业分类
电工技术 | 24篇 |
综合类 | 192篇 |
化学工业 | 1972篇 |
金属工艺 | 46篇 |
机械仪表 | 209篇 |
建筑科学 | 160篇 |
矿业工程 | 34篇 |
能源动力 | 32篇 |
轻工业 | 1592篇 |
水利工程 | 13篇 |
石油天然气 | 140篇 |
武器工业 | 23篇 |
无线电 | 105篇 |
一般工业技术 | 179篇 |
冶金工业 | 77篇 |
原子能技术 | 14篇 |
自动化技术 | 166篇 |
出版年
2024年 | 9篇 |
2023年 | 25篇 |
2022年 | 89篇 |
2021年 | 175篇 |
2020年 | 110篇 |
2019年 | 99篇 |
2018年 | 132篇 |
2017年 | 177篇 |
2016年 | 176篇 |
2015年 | 238篇 |
2014年 | 334篇 |
2013年 | 345篇 |
2012年 | 369篇 |
2011年 | 379篇 |
2010年 | 300篇 |
2009年 | 268篇 |
2008年 | 244篇 |
2007年 | 240篇 |
2006年 | 211篇 |
2005年 | 186篇 |
2004年 | 183篇 |
2003年 | 133篇 |
2002年 | 126篇 |
2001年 | 101篇 |
2000年 | 76篇 |
1999年 | 82篇 |
1998年 | 53篇 |
1997年 | 47篇 |
1996年 | 12篇 |
1995年 | 26篇 |
1994年 | 14篇 |
1993年 | 5篇 |
1992年 | 5篇 |
1991年 | 3篇 |
1990年 | 3篇 |
1989年 | 2篇 |
1980年 | 1篇 |
排序方式: 共有4978条查询结果,搜索用时 15 毫秒
1.
在传统的轮胎表面缺陷依靠人工检测,存在劳动强度高、受人的主观影响大以及效率低下的问题。针对这一现象,研究了一种基于机器视觉的轮胎表面缺陷3D检测系统。该系统依靠机器视觉系统获取检测轮胎的表面图像,然后创建3D模型、判定缺陷类型,最终实现实时自动预警,为轮胎生产商提供一种自动化检测方案。系统集成了先进的技术、软件和工具,配套的信息管控系统可以对轮胎型号和生产数据进行采集、存储、分析,以便在生产过程中实现更高效、更可靠的质量控制,具有较高的实际应用推广价值。 相似文献
2.
《Food Control》2015
Bacillus cereus can cause emetic and diarrheal food poisoning. It is widespread in nature and therefore, considered a major foodborne pathogen. To develop a sensitive and reliable assay for detecting enterotoxin genes (nheA, entFM, hblD, cytK) and emetic toxin (ces), specific primers each targeting one individual gene were designed. Propidium monoazide (PMA) was coupled with the developed multiplex PCR (mPCR) for the detection of viable B. cereus. The inclusivity and exclusivity of the PMA-mPCR was confirmed using a panel of 44 strains including 17 emetic and 9 enterotoxic B. cereus reference strains and 18 non-target strains. The limit of detection (LOD) without PMA treatment in pure DNA was 2 pg/reaction tube. The LOD of mPCR assay in pure heat-killed dead bacteria was 4.0 × 102 CFU/mL. Also, the LOD on the viable bacteria with or without PMA treatment was similar (3.8 × 102 CFU/mL) showing that the PMA treatment did not significantly decrease sensitivity. Finally, the newly developed PMA-mPCR successfully detected 4.8 × 103 and 3.6 × 103 CFU/g of viable B. cereus F4810/72 (emetic) and B. cereus ATCC 12480 (enterotoxic) reference strains, respectively, in food samples. Hence, this study combines PMA and mPCR to detect viable B. cereus with a wide range of toxin detection (5 toxins). Thus, the novel PMA-mPCR assay developed in this study is a rapid and efficient diagnostic tool for the monitoring of viable B. cereus in food samples and potentially other samples via appropriate DNA extraction. 相似文献
3.
4.
《Food Control》2015
A diverse range of genetic elements has been used to develop genetically modified organisms (GMOs) over the last 18 years. Screening methods that target few elements, such as the Cauliflower Mosaic Virus 35S promoter (P-35S) and Agrobacterium tumefaciens nopaline terminator (T-nos), are not sufficient to screen GMOs. In the present study, a multiplex PCR system for all globally commercialized GM soybean events was developed to easily trace the events. For this purpose, screening elements of 24 GM soybean events were investigated and 9 screening targets were selected and divided into three individual triplex PCR systems: P-35S, ribulose-1,5-bisphosphate carboxylase small subunit promoter of Arabidopsis thaliana, T-nos, T-35S, pea E9 terminator, open reading frame 23 terminator of A. tumefaciens, proteinase inhibitor II terminator of potato, acetohydroxy acid synthase large subunit terminator of A. thaliana, and the revealed 3′ flanking sequences of DP-305423-1. The specificity of the assays was confirmed using thirteen GM soybean events as the respective positive/negative controls. The limit of detection of each multiplex set, as determined using certified reference materials of specific GM events, ranged from 0.03 to 0.5%, depending upon target. Furthermore, 26 food samples that contained soybean ingredients, which were purchased from the USA, China, Japan, and Korea, were analyzed, 17 of which contained one or more GM soybean events. These results suggest that the developed screening method can be used to efficiently track and identify 24 GM soybean events in food and feed. 相似文献
5.
Maria Grazia Basanisi Gianfranco La Bella Gaia Nobili Rosa Coppola Annita Maria Damato Maria Assunta Cafiero Giovanna La Salandra 《International Journal of Food Science & Technology》2020,55(3):1145-1150
The authenticity and traceability of meat products are issues of primary importance to ensure food safety. Unfortunately, food adulteration (e.g. the addition of inexpensive cuts to minced meat products) and mislabelling (e.g. the inclusion of meat from species other than those declared) happens frequently worldwide. The aim of this study was to apply a droplet digital PCR assay for the detection and quantification (copies μL−1) of the beef, pork, horse, sheep, chicken and turkey in meat products. The analysis conducted on commercial meat showed the presence of traces of DNA from other animal species than those declared. We show that the method is highly sensitive, specific and accurate (accuracy = 100%). This method could be adopted by competent food safety authorities to verify compliance with the labelling of meat products and to ensure quality and safety throughout the meat supply chain, from primary production to consumption. 相似文献
6.
7.
8.
文章简要阐述了PID码在多节目码流的复用与解复用中所起的作用。并运用PID码的原理来解释和解决在实际工作中碰到的PID码问题。 相似文献
9.
采用轴向式气胎离合、制动器,改造了旧飞剪,解决了原剪机电磁离合、制动器控制不准确的问题;该剪机改造后在成品轧机后使用,剪切用120mm×120mm坯生产的大规格产品(18mm以上),增产、增效,年总经济效益为97.8万元 相似文献
10.
27例乙型血友病患者Ⅸ因子基因突变研究 总被引:1,自引:0,他引:1
采用PCR(聚合酶链反应)及GAWTS(GenomicAmplificationwithTranscriptsSequencing)技术,研究了江苏、湖北、山东、广东、福建、宁夏六省区27例乙型血友病患者及其家系成员FⅨ(九因子)基因各2.2Kb DNA序列。在2l例中发现20种不同类型的突变。其中12种是未曾报道的新突变。38%的突变发生在CpG二核苷致序列上,进一步证实了CpG确系突变热点。同时检出6例女性为致病基因携带者。对开展基因产前诊断及优生优育等,具有重要意义。 相似文献