电泳漆二次流挂性检测方法及其应用

介绍了一种电泳漆二次流挂性能的试验方法,并在汽车设计阶段进行涂装同步工程时用于分析产品设计缝隙大小对电泳漆流痕状况的影响。     

Separation and characterization of mares' milk alpha(s1)-, beta-, kappa-caseins,gamma-casein-like,and proteose peptone component 5-like peptides

The equine alpha(s1)- and beta-caseins (CN) were purified by chromatography on DEAE-cellulose and by reversed-phase HPLC. The alpha(s1)-, beta-, and kappa-CN were characterized either by monodimensional urea-PAGE or sodium dodecylsulfate (SDS)-PAGE or by bidimensional electrophoresis. Kappa-casein was characterized after electrophoresis by glycoprotein-specific staining. To identify alpha(s1)-CN without ambiguity, internal sequences were determined after trypsin or chymosin digestion of purified alpha(s1)-CN. These sequences, that could be estimated to correspond to 62% of the full protein, presented strong identities with regions of alpha(s1)-CN primary structures of other species. In particular, 51, 48, 43, and 40% identities were obtained with corresponding regions of sow, dromedary, cow, and human alpha(s1)-CN, respectively. On the other hand, trace amounts of equine gamma-CN-like and proteose peptone component 5-like peptides were found in the whole CN. They were identified by microsequencing and corresponded to beta-CN peptides generated by plasmin action on the whole CN. The equine alpha(s1), beta-, and kappa-CN were separated by bidimensional electrophoresis in numerous isoelectric variants with apparent isoelectric points distributed between pH 4.4 to 6.3, 4.4 to 5.9, and 3.5 to 5.5, respectively. The beta- and kappa-CN displayed a more acidic character in the mare than in the cow.     

用镁牺牲阳极防三相分离器内壁腐蚀的研究

针对大庆天然气公司某大队实际工矿条件下的压力容器内壁腐蚀问题,用柴油、乙二醇和水配制成模拟的混合相体系,借助静态、动态法进行了腐蚀试验,利用极化曲线法测定了体系的开路电压、腐蚀电位、腐蚀电流密度、腐蚀速率、最小保护电位和最小保护电流密度.结果16 MnR钢片的开路电位为-610.0 mV,腐蚀电位为-631.0 mV,腐蚀电流密度为7.680 μA/cm2;镁阳极开路电压为-1.507 V,腐蚀电位为-1.470 V,腐蚀电流密度为11.200 μA/cm2.确定最小保护电位为-0.981 V,最小保护电流密度为110.700 μA/cm2,镁阳极的实际保护电位为-1.200 V左右.结果证明,采用牺牲阳极法可以对该容器内壁实现保护作用.     

混凝-陶瓷膜微滤处理阴极电泳漆废水研究

提出用混凝与陶瓷膜微滤组合工艺处理阴极电泳漆废水的方法,研究了pH值对CODCr去除率的影响,操作压差、膜面流速对膜通量的影响,得到了适宜的操作条件和有效的膜清洗方法.结果表明:料液pH值为6.7时,混凝过程对CODCr去除率的贡献约63%,之后的微滤使废水中CODCr的去除率增加到85%.采用平均孔径为0.2μm的氧化锆膜,在膜面流速4.2 m/s,压差0.10 MPa,温度30℃,膜稳定通量约250 L/(m2.h),渗透液可作为涂装车间循环冲洗水用.混凝作为前处理过程有助于提高膜的渗透通量,降低膜污染,改善出水水质.     

磁过滤真空弧源沉积C/C多层复合膜的结构和力学性能研究

采用磁过滤直流阴极真空弧源沉积技术在不锈钢基体表面制备了C/C多层复合膜,通过X射线光电子能谱、Raman光谱对薄膜的结构进行表征;C/C多层膜大气下的摩擦损性能在销盘式摩擦磨损试验机上进行;用洛氏压痕法研究了薄膜与基体的结合强度.结果表明：C/C多层复合膜为类金刚石结构.它与SiC球大气下的摩擦系数为0.10左右,其摩损性能由于多层膜的引入而显著提高.Ti过渡层的引入显著提高了膜基结合力.     

多肽在毛细管区带电泳的迁移模型

提出了一个多肽在毛细管区带电泳的迁移模型．该模型显示出，多肽的迁移时间是其电荷、相对分子质量、构象参数（Ｒｃ）以及电泳操作条件参数（Ａ）的函数．引入的构象参数表征了生物大分子的形状多样性，由于生物大分子种类的多样性和其构象的复杂性，其构象参数应是大于１／３的实数点的集合，参数Ｒｃ和Ａ可从实验数据拟合求得，测定了在ｐＨ＝２．５的缓冲溶液中，不同实验条件下的九种标准多肽物质的毛细管区带电泳的迁移时间，实验数据与本文的理论预计一致．     

线性浓度梯度SDS—PAGE在小麦蛋白质研究中的应用

本研究发现线性浓度梯度SDS—PAGE在小麦蛋白质研究方面具有传统SDS—PAGE难以比拟的优越性,其表现是:适用范围广,能在同一系统对分子量相差很大的多个样品进行分析研究,并可对分子量进行测定;持续的样品浓缩效应使电泳图谱更清晰,分辨率提高;特别对混合组分中高分子部分效果好,且重现性高;梯度混合器装配简便,普通实验室即可组装,在小麦品质分析和小麦品种鉴定上具有广泛的应用价值。     

Co2+离子对Zn电积阴极过程的影响

采用多种电化学研究方法探讨了在Zn电积过程中Co^2 对阴极极化过程的影响,并对其极化机理进行了研究．研究表明,当Co^2 离子≥6mg时,产生严重的“烧板”现象,降低了阴极Zn沉积反应的交换电流密度．对Zn沉积反应起极化作用,而且严重影响Zn的晶体形状．     

在役埋地管线涂层质量评价方法

提出了利用“针眼指数”来评价涂层质量的方法 ,建立了相关的数学模型和评价等级 ,并结合现场检测情况对该方法进行了验证。该方法开拓了管道涂层质量控制的新思路 ,它比常规方法方便可行 ,为采用阴极保护的埋地管道涂层质量评价提供了一种有效的手段。     

Correlations between substrate bias, microstructure and surface morphology of tetrahedral amorphous carbon films

The microstructure and surface morphology of ta-C films deposited on p-type (1 0 0) single crystal silicon with the substrate negative bias varying from 0 to 2000 V by the filtered cathodic vacuum arc technology have been investigated by means of Raman spectroscopy and atomic force microscope. The optimal deposition process of sp³-rich ta-C films can be confirmed in light of the relations between the coupling coefficients or full-width at half-maximum and the substrate negative bias. The surfaces of these films are uniform and smooth and RMS surface roughness is less than 0.4 nm. At the lower energetic grades, the more the content of sp³ is in the film, the smoother the surface of the film is. The dependence of the surface morphology and the impinging energy of the species can be illustrated according to the subimplantation growth mechanism. Nevertheless at the high energetic grade, the impinging ions with appropriate energy sputter and smoothen the surface so that the roughness might be even lower than the one of the films with the richest sp³ component.