应用固定化细胞改善酱油风味的探讨

为了改善酱油风味，首先阐明了传统发酵和固定化细胞发酵的各自特点。说明传统酱油酿造是多茵种混合发酵，酱油风味好。介绍了固定化细胞发酵酱油：采用固定化细胞发酵提高发酵率，缩短发酵周期，固定化细胞可长期使用，降低了成本，避免污染，产品质量高。介绍了国内外对固定化细胞发酵法的研究情况。提出采用固定化细胞和传统多茵种混合发酵相结合，从而改进固定化细胞发酵法，可进一步提高酱油风味。     

高效液相色谱法测定酱油中苯甲酸含量

为了准确地测定酱油中苯甲酸含量,采用高效液相色谱法,以甲醇:乙酸铵溶液(0.02 mol/L)=5:95为流动相,总流速1.0 mL/min测定酱油中苯甲酸含量,此方法具有定量准确、精密度高和操作方便等特点.     

发酵型猪骨调味料的研制

利用瑞士乳杆菌发酵猪骨酶解液制备发酵型猪骨调味料。结果表明,发酵工艺条件为液体种子种龄9h、接种量9%、发酵温度40℃、发酵时间18h,得到的发酵液苦味消失、氨基态氮含量增加、猪骨风味增强。此发酵液经调配得到发酵型猪骨调味料。     

酱油发酵过程中的耐盐乳酸菌筛选及对低盐固态酱油品质的影响

本文通过对酱醪中耐盐乳酸菌的筛选,成功分离出一株耐18%盐度的乳酸菌,经过16S r DNA测序鉴定,确定为一株植物乳杆菌。在酱油酿造过程中添加该耐盐植物乳杆菌,无盐固形物、氨基酸态氮、还原糖和总酸含量等理化指标都有所上升。经过液相测定,发现酱油中乳酸含量比空白增加2倍左右。挥发性风味物质中酮类和酚类物质增加2倍左右,醇类物质稍有增加。本研究为进一步安全有效提高酱油风味物质奠定理论依据。     

苦瓜浸提物对豆瓣酱防腐作用的研究

从腐败的豆瓣酱中分离获得了1株细菌和1株酵母菌；进行了苦瓜提取物对它们的抑制作用实验，并做了苦瓜提取物用于豆瓣酱防腐的应用试验，研究了添加不同浓度苦瓜提取物对豆瓣酱风味的影响。     

Improvement of the in vitro digestible iron and zinc content of okra (Hibiscus esculentus L.) sauce widely consumed in Sahelian Africa

ABSTRACT:  The effects of the formulation (okra, fish, soumbala, extract of wood ash) and cooking time of okra sauce on total iron and zinc content and on their in vitro digestibility were evaluated following a Doehlert uniform shell design with 5 factors and 33 trials. Cooking time had no significant effect on in vitro digestible iron and zinc content, whereas formulation did. Each ingredient had a specific effect. Extract of wood ash, which is a source of soluble and digestible iron and zinc, is a good way of increasing the digestible mineral content of the dish. Okra, the main ingredient in this sauce, has a negative effect and should be added in moderate quantities (< 37.7% of the DM of the sauce). An optimization using the desirability function allows us to identify the optimal recipe that enabled the quantity of digestible iron to be doubled and the quantity of digestible zinc to be increased by one third. This recipe calls for a mixture of 37.7% okra, 26.3% dried fish, 18.5% soumbala, and 3.7% extract of wood ash cooked for 25 min.     

酱油用圆盘制曲机自动控制系统的应用

针对圆盘制曲机专门开发的CHR-Process600智能控制系统，可实现从原料至成品全过程的标准化作业，达到真正的在封闭式状态下，做到自动化生产高质量的酱油曲，减少人为因素的影响。该文介绍了CHR-Process600圆盘制曲机自动控制系统的结构、功能以及在实际生产中应用的效果。     

花椒麻辣凉拌调味汁的生产工艺

花椒是一种独特的香料，在麻辣凉拌调味汁中发挥了不可缺少的作用，本文主要就花椒麻辣凉拌调味汁的生产工艺、产品质量指标等方面进行了研究。     

NaCl-activated extracellular proteinase from Virgibacillus sp. SK37 isolated from fish sauce fermentation

ABSTRACT:  Virgibacillus sp. SK37 exhibited high extracellular proteolytic activity in skim milk broth containing 10% NaCl. Optimum conditions of the crude proteinase were at pH 8.0 and 65 °C. The proteinase was strongly inhibited by phenylmethanesulfonyl fluoride (PMSF) and preferably hydrolyzed Suc-Ala-Ala-Pro-Phe-AMC, suggesting the serine proteinase with a subtilisin-like characteristic. Proteolytic activity increased with NaCl concentration up to 20%. Ca²⁺ activated the enzyme activity but reduced enzyme stability at 65 °C. Several proteinases with dominant molecular mass (MW) of 81, 67, 63, 50, 38, and 18 kDa were detected on native-polyacrylamide gel electrophoresis (native-PAGE) activity staining in the absence and presence of 25% NaCl. These results demonstrated that Virgibacillus sp. SK37 produced salt-activated extracellular proteinases. Virgibacillus sp. SK37 could be a promising strain for starter culture development used in fish sauce fermentation.     

准确检验头油氨基酸态氮含量的重要作用

在食品质量安全准入市场制度下，国家规定酱油出厂要作8项以上检验合格方准入市。按国家标准GB18186-2000准确检验头油中氨基酸态氮含量占酱油出厂8项检验合格具有十分重要作用与地位，特别是它与4项质量技术指标检验合格还具有纲举目张的连带关系。