乙肝病毒融合表面抗原SA—28基因在酵母中的表达

利用基因工程技术，先将乙肝病毒表面抗原Ｓ－ｐｒｅＳ１融合基因ＳＡ－２８置于酵母杂合启动子ＡＤＨ２－ＳＵＣ２的控制下，然后将ＳＡ－２８基因的表达单元插入高稳定质粒ｐＨＣｌ１的ＢａｍＨＩ位点，构建成表达质粒ＹＦＤ１５０和ＹＦＤ１５０－ｏ并将其转化酿酒酵母Ｙ１９。对转化子表达ＳＡ－２８基因的研究表明：表达受葡萄糖浓度调控；表达产物具有Ｓ和ｐｒｅＳ１双重抗原性，并形成密度为１．２０－１．２２ｇ／ｍｌ的颗粒     

天山根瘤菌模式菌株全序列测定及其系统发育

对天山根瘤菌群的模式株Ａ－ＩＢＳ１６ＳｒＤＮＡ的全序列进行了测定，以确定它在系统发育中的地位。实验中设计了６个引物，通过聚合酶链反应扩增天山根瘤菌模式菌株Ａ－ＩＢＳ的１６ＳｒＤＮＡ。应用ＤＮＡ定向测序技术，获得了菌株Ａ－ＩＢＳ的１６ＳｒＤＮＡ全序列，将所得序列与根瘤菌已知种的模式菌株１６ＳｒＤＮＡ全序列一起进行聚类分析，得到了系统发育树状图，在树状图中，菌株Ａ－ＩＢＳ所代表的根瘤菌群与华癸根瘤菌群     

醋酸钙不动杆菌PHEA-2的16S rDNA测序及其苯酚降解特性研究

从我国东北某炼油厂工业废水中分离到一株高效降解苯酚的菌株ＰＨＥＡ－２,采用ＰＣＲ技术从该菌总ＤＮＡ中克隆到１５００ｂｐ的１６Ｓ ｒＤＮＡ片段。该片段的核苷酸序列分析结果表明,ＰＨＥＡ－２的１６Ｓ ｒＤＮＡ核苷酸序列与醋酸钙不动杆菌模式菌株的同源性为９８％。在细菌系统发育分类学上,ＰＨＥＡ－２归属变形细菌γ亚类中的不动杆菌属,醋酸钙不动杆菌,ＰＨＥＡ－２除了能够降解苯酚外,还能降解苯甲酸盐和邻苯二酚     

人GM－CSF在链霉菌中的表达

报道了ＰＩＪ４５９／ＧＭ－ＣＳＦ基因表达质粒的构建，以及该重组质粒在变铅青链霉菌Ｓ．ｌｉｖｉｄａｎｓＴＫ５４中的表达，结果表明：ＰＩＪ４５９／ＧＭ－ＣＳＦ质粒具有表达ｈＧＭ－ＣＳＦ的功能。重组菌株细胞裂解液经ＴＦ－１细胞培养及集落形成实验均证实了其生物学活性。并分析了影响外源基因表达的某些因素，如ＳＤ序列与ＡＴＧ之间的距离，时间对表达的诱导等，探讨了外源基因高效表达的适宜条件。     

高表达重组人粒细胞集落刺激因子cDNA的中国仓鼠卵巢细胞株的建立

用质粒ｐＥＦ－ＢＯＳ的增强子、肽链延长因子基因的启动子及其第一内含子替换质粒ｐＥＤ－ＧＣＳＦ的增强子，腺病毒主要晚期启动子及杂合内含子。构建成了更高表达质粒ｐＥＦ－ＧＣＳＦ，转染ＣＨＯ－ｄｈｆｒ－细胞，加入并逐渐升高氨甲喋呤的浓度。结果显示：一定范围内，随着ＭＴＸ浓度增高，重组人粒细胞集落刺激因子表达量随之提高；筛选并建立了一株稳定高表达ｒｈＧ－ＣＳＦ ｃＤＮＡ的细胞株，其表达量为５．０￣５．６μ     

内含子对人凝血因子IX活体表达的增强作用

为了研究内含子在活体水平对凝血因子Ⅸ的表达增强作用，首先采用ｌａｃＺ为报告基因，建立了阳离子脂质体ＳＡ介导的活体基因转移方法。将带有凝血因子Ⅸ（ｈＦⅨ）ｃＤＮＡ的质粒ｐＣＭＶＩＸ和带有ｈＦⅨｃＤＮＡ及其内含子的小基因的质粒ｐＣＭＶｉ＇Ⅸ用ＳＡ脂质体包裹，分别经尾静脉注射到Ｂａｌｂ／ｃ鼠体内，ｐＣＭＶＩＸ注射后未在血浆中检测到ｈＦⅨ蛋白；而ｐＣＭＶｉ＇Ⅸ注射后在Ｂａｌｂ／ｃ鼠血浆中检测到ｈＦⅨ蛋白，     

热休克后表达受抑基因的mRNA差别显示和克隆

以人成骨肉瘤细胞株（ＨＯＳ－８６０３）作为热休克模型，用差别显示ｍＲＮＡ法（ｄｄｍＲＮＡ）筛选，发现和克隆了一个热休克后表达受抑的ｃＤＮＡ片段。在４３℃、４０分钟热处理后１小时，ＨＯＳ－８６０３细胞总ｃＤＮＡ图谱未见明显变化，但以该ｃＤＮＡ片段作为探针，ＲＮＡｄｏｔｂｌｏｔ和Ｎｏｒｔｈｅｒｎｂｌｏｔ证实，热休克后该基因的ｍＲＮＡ的量明显下降，而ｍＲＮＡ未见明显降解。我们将这个因热休克而选择性的表达受抑的基因命名为ＨＳＳＧ－１。对全长３１２ｂＰ的ｃＤＮＡ片段的序列分析表明，ＨＳＳＧ－１可能是一个未知的新基因。     

酵母人工染色体载体的改造和修饰

将能在植物中表达的烟草花叶病毒ＣａＭＶ３５Ｓ启动子、ＧＵＳ基因、潮霉素（Ｈｙｇｒｏｍｙｃｉｎ）基因及ＭＯＳ终止子组建到酵母人工染色体（ＹＡＣ）载体，构建了具有能在植物细胞中表达和选择ｐＹＡＶ、ＧＵＳ、ｐＪＳ９７／ｐＪＳ９８－ＨＹ ＹＡＣ载体系体系统和一个标记ＹＡＣ克隆的ｐＵＲ４３－ＨＹ质粒。     

肌肉介导心钠素基因对L-NNA高血压大鼠的作用

用重组人心钠素基因真核表达质粒ｐｃＤＮＡ３／ＡＮＦ，通过基因缝线转移至Ｌ－ＮＮＡ高血压大鼠股四头肌，发现可明显降低Ｌ－ＮＮＡ引起的高血压，并产生明显的利钠利尿作用。提示肌肉介导心钠素基因，作为一种ＤＮＡ药物治疗高血压，可能具有潜在应用价值。     

颗粒增强Fe3Al基复合材料的制备和性能

用熔铸法制备了ＳｉＣ,Ａｌ２Ｏ３和ＴｉＢ２颗粒增强Ｆｅ－２８Ａｌ－５Ｃｒ基复合材料,研究了材料的微观组织和力学性能,结果表明,Ａａ２Ｏ３在Ｆｅ３Ａｌ中的化学稳定性很好,ＴｉＢ２与基体发生了部分反应,ＳｉＣ与基体的反应严重,复合材料在室温和高温下的力学性能的变化说明,上述颗粒的加入使材料的强度较大幅度的提高,塑性有所下降。     

The Research of Anti-Wear Properties of Organomolybdenum Compounds in the Engine Oils

A four-ball tester was used to evaluate the anti-wear performance of three kinds of organomolybdemun compounds in the engine oils, i. e., molybdenum dialkyldithiophosphate (MoDDP), molybdenum dialkyldithiocarbamate ( MoDTC), and sulphur and phosphorus freeorganomolybdeum (Molybdate). The results indicate that a low concentration of MoDDP doesn' t improve the anti-wear properties of the commercial engine oils, but a high concentration of MoDDP can obviously improve the anti-wear properties and the load-carrying capacity of the engine oils. MoDTC doesn' t improve the antiwear properties of the engine oils, but worsens the anti-wear properties of the oils. Signifi can timprove ment of frictional and wear characteristics is obtained with Molybdate added in the commercial engine oils and the formulated oils.     

Effect of Permeable Crystalline Material on Steel Reinforcement Corrosion of Concrete

Permeable crystalline materialcan permeate into pores and cracks of concrete and catalyze the reaction between Ca(OH) 2and unhydrated cement to generate a great quantity needle non-soluble crystals, which can stop up the pores and cracks of concrete, and increase the impermeability of concrete. This paper reported the results of a study conducted to evaluate steel reinforcement corrosion of concrete specimens uncoated and coated with permeable crystalline material as well as mixed with the permeable crystalline material. The properties evaluated for corrosion test were water impermeability, water absorption, compressive strength and potential. The results of water impermeability, water absorption, compressive strength clearly showed that the permeable crystalline material could prohibit water, any soluble salts and moisture from penetrating the concrete to cause corrosion, leaking, and other problems, and it did increase the compressive strength, which was favorable for protection of corrosion of reinforcing steel. Moreover, it was concluded from the potential-time curve that the steel reinforcement of uncoated specimen was in the state of activation whereas that of other specimens coated and mixed with the permeable crystalline material was in the state of inactivation. Above all, it was indicated that the permeable crystalline materialis very effective to protect the steel reinforcement of concrete from corrosion.     

The Research and Application About "Dry Peeling Off" Technology for Airplane Surface Paint Layer

In this paper, the principle of spurting take off lacquer were introduced; and with orthogonal method the unsaturate polyester was prepared ;it was easy to peel airplane paint layer off with this material and equipment.     

Quality Study of Honeycomb Core Shaping Without Wax Stabilization

Sandwich panels utilizing honeycomb cores result in lightweight, high-strength units that are very rigid. Indeed, they give honeycomb panels the best strength-to-weight ratio of any construction type available. Conventionally, the wax is poured into the cell of the core to strengthen the structure and prevent the core from being crushed during milling. Much time and labor is required to wax, mill, and clean the cores. In this study, the process of pouring wax into the core was skipped. In other words, the cutter directly milled the core. Experiments showed that the compressive strength of the core produced without wax stabilization was only 3% lower than that of cores produced using the traditional method. In addition, the drum peel strengths of cores produced without wax stabilization were 38.4% higher than cores produced using the traditional method, in which residual wax seriously polluted the adhesive. It was also found that eliminating the wax process can reduce costs.     

Certification Becomes Important for the I Quality; and Safety Supervision of the Chinese Liquor Sector

The end of 2007, over 200 unit products of more than 80 Chinese firms have passed the quality grade certification for liquor products. These products involve distilled spirits, beer, wine, yellow wine, fruit syrup wine and others, and cover over 80% of the national top-branded liquor products.……     

Deposition and Coating Properties on CVD Tungsten

Surface characterization and microstructure studies are performed on chemical vapor deposited (CVD) tungsten coating. There is about 2 μm thickness diffusion layer of tungsten in the molybdenum substrate. The thermal shock test shows tungsten coating has good adhesion with molybdenum substrate, but the elements of oxygen and carbon in the tungsten coating have the bad affection to the adhesion. The result of high-temperature diffusion experiment is the diffusion rate from molybdenum substrate to tungsten coating is faster.     

Analysis of marine hydrobiont lipid extracts as possible cryoprotective agents

We studied a combined effect of the cryoprotectants both of lipid and carbohydrate origin at the presence of DMSO or without it on cell viability and the RNA synthesis in the embryonic mollusc and echinoderm cell cultures obtained at different developmental stages. Cryoprotective properties of exogenous lipids correlated with their thermotropic behavior. Lipid extracts from marine hydrobiontes, which thermal transitions are characterized by relatively small part of calorimetric thermogram area lying at temperatures above 0 °C (6–18%), were found to possess a considerable cryoprotective activity. The results are discussed in relation to the prediction of the cryoprotective activity of lipid extracts for marine animal cells. In addition, it was found that marine invertebrate cells can be cryopreserved without DMSO at the presence of trehalose and lipid extracts of some marine hydrobiontes.     

In-process thermal treatment of polylactic acid in fused deposition modelling

Polylactic acid (PLA) is one of the most widely used open source fused filament fabrication materials due to its ease of extrusion, biodegradability, and mechanical strength. The mechanical strength of PLA largely depends on the proper growth of its semi-crystalline structure, which can be severely impaired by a low rate of crystallization, particularly in open source printers. This can be further aggravated by the non-uniform thermal distribution of heat that causes improper curing among the extruded beads of the printing material. As a result, PLA printed on open source printers does not achieve the best mechanical properties. This research, for the first time, proposes an additive-free solution implemented through a detailed set of experimentation to improve the curing rate through in-process temperature variations to cure the joints among the beads. Scanning electron microscopy (SEM), differential scanning calorimetry (DSC) and Fourier transform infrared (FTIR) spectroscopy is used to confirm the improvements in the bead joints. This work is conducted in two phases of experiments. In the first phase, a full factorial ANOVA is used to investigate various process parameters and the important variables are used in the second phase to print test specimens in four different sets.     

Low-Temperature Properties of Silver

Pure silver is used extensively in the preparation of high-temperature superconductor wires, tapes, films, and other configurations in which the silver not only shields the superconducting material from the surrounding materials, but also provides a degree of flexibility and strain relief, as well as stabilization and low-resistance electrical contact. Silver is relatively expensive, but at this stage of superconductor development, its unique combination of properties seems to offer the only reasonable means of achieving usable lengths of conductor. In this role, the low-temperature physical (electrical, thermal, magnetic, optical) and mechanical properties of the silver all become important. Here we present a collection of properties data extracted from the cryogenic literature and, to the extent possible, selected for reliability.     

Early ironwork in Biscay: Survey,excavation, experimentation and materials characterization. An integral study of the mountainside ironworks (ferrerías de monte or “haizeolak”)

ABSTRACT

The production of iron in Biscay (Basque Country, Spain) has been a hallmark of this territory of the North of the Iberian Peninsula throughout its history. The Archaeology team of the Basque Country Mining Museum has spent 13 years, so far, studying the origins of this activity through the evidences left at the old pre-hydraulic iron production workshops known as mountainside ironworks (ferrerías de monte) or haizeolak. It has catalogued 170 archaeometallurgical sites of this type in Biscay, and the archeological excavation of some of them has led to the knowledge of the different stages of the production process that took place in these workshops. The team has also experimentally reproduced the work carried out in a mountainside ironworks from the High Middle Ages, and it has currently started the characterization process of this type of activity through the study of the collected materials.