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1.
太空诱变玉米核不育基因的SSR作图   总被引:3,自引:0,他引:3  
为研究太空诱变玉米核不育材料的不育基因所在位点,以雄性不育材料03SRP9×S37(自交系)的F2代群体作为育性调查和SSR作图群体,用玉米第3染色体3.06位点的37对SSR引物进行差异筛选,结果表明分子标记umc1674,umc2076,umc1644,dupssr17,bnlg197,umc2271,umc1951和bnlg1047与不育基因表现为连锁遗传,进一步将不育基因定位在分子标记umc2076和umc1674之间,遗传距离分别为4.4 cM和3.7 cM,并以此为基础构建了核不育基因区间的遗传连锁图,为下一步克隆该基因奠定了基础.  相似文献   

2.
AFLP分子标记构建中国对虾遗传连锁图谱的初步研究   总被引:29,自引:1,他引:28  
利用AFLP分子标记结合“拟测交”策略,以中国对虾的单对杂交亲本及其F1代为作图群体,应用MAPMAKER EXP/3.0软件,分别构建了中国对虾雌、雄的遗传连锁图谱。在中国对虾的雄性连锁图谱中,74个标记组成了5个连锁群(遗传标记数量大于3)、7个三联体、13个连锁对,总图距为951.5eM,最大连锁群的长度为206.2eM,最短的为7.4eM,标记间的平均距离为12.8eM;雌性连锁图谱中,由66个标记组成的5个连锁群,4个三联体,13个连锁对,总图距为712.7eM,连锁群的长度介于7.7eM~128.2eM之间,标记间的平均距离为10.7eM。估算的中国对虾基因组总长度为2000eM,所构建的连锁图谱分别覆盖了基因组长度的47.5%和35.6%。中国对虾遗传连锁图谱的构建为分子标记辅助育种、比较基因组作图以及基因定位与克隆创造了条件,并对今后其他海洋生物遗传图谱的构建具有理论价值和实践意义。  相似文献   

3.
天鹅洲保护区长江江豚AFLP遗传多样性分析   总被引:7,自引:0,他引:7  
应用AFLP技术对天鹅洲白豚国家级自然保护区的长江江豚 (Neophocaenaphocaenoidesasiaeorientalis)群体进行了遗传多样性分析。每个AFLP引物组合在群体中检测到 0到 6个不等的多态性片段 ,平均为 2 .4 8个。 2 1个引物组合共揭示了 1139个座位 ,其中 5 2个座位具有多态性。多态性座位所占比例为 4 .6 %。分析显示群体的遗传期望杂合度 (He)为 0 .4 430± (SE0 .0 12 2 2 )。研究结果表明保护区长江江豚群体具有适度的核DNA遗传多样性水平。为了该群体的长期生存与发展 ,更多的具有不同遗传变异的个体应该被迁入保护区 ,以形成更大的群体 ,获得更好的群体遗传结构  相似文献   

4.
电镀     
20 0 2 0 70 1 ZnCl2 NaCl(摩尔分数 4 0 %~ 6 0 % )熔盐电沉积W———MasudaM .JElectrochemSoc,2 0 0 1,14 8(1) :5 9(英文 )用伏安法研究了含不同W化合物的ZnCl2 NaCl(摩尔分数4 0 %~ 6 0 % )熔盐在 4 5 0℃下W的电沉积 ,在恒定电位下 ,使用含WO3、K2 WO4 、WCl4 、K2 WCl6 的镀槽可以获得金属W ,但含K3W2 Cl9和WCl2 的镀槽不能获得金属W层。2 0 0 2 0 70 2 Sn Zn合金电镀———RasmussenJ .Plating&SurfaceFin ,2 0 0 1,88(2 ) :…  相似文献   

5.
用溶胶 -凝胶技术控制硅凝胶颗粒的粒度 ,向烧结金属钛的微孔内嵌入不同粒度的凝胶颗粒 ,经 6次涂敷、陈化和烧结后 ,制成微孔金属钛 -SiO2 复合膜 ;将该膜用于超滤分离聚乙二醇 (PEG)和玉米蛋白水溶液 ,在过滤压差Δp =0 .3MPa时 ,对分子量 2 0 0 0 0的聚乙二醇 ,其稳定通量JP=8.4mL/ (cm2 ·h) ,截留率RP=92 .8%~ 93.4 % ;对玉米蛋白 ,其稳定通量JA=6 .7mL/ (cm2 ·h) ,截留率RA=95 .2 %~ 95 .6 % .  相似文献   

6.
采用共沉淀方法,以Gd2O3、Al(NO3)3·9H2O、Nd2O3(Yb2O3)为原料、氨水为沉淀剂,合成了Nd:GdAlO3(5%(原子分数))和Yb:GdAlO3(5%(原子分数))纳米多晶粉末,采用X射线衍射(XRD)和Rietveld精修方法对晶体结构进行了研究,采用场发射扫描电镜(FESEM)对晶体的形貌进行了研究,同时对晶体的发射光谱进行了测量.XRD结果表明,在较低的煅烧温度和较短的煅烧时间下(1000℃,保温3h)可获得纯Nd:GdAlO3和Yb:GdAlO3晶相,同时晶粒尺寸随着煅烧温度的升高而增大.Rietveld精修结果表明半径较大的Nd3 离子的掺杂,使GdAlO3晶体结构有从正交相向四方相转变的趋势.FESEM结果表明,颗粒大小均在纳米尺寸范围之内,且具有较好的分散性.在Nd:GdAlO3晶体的发射光谱中,在927、1077和1335nm附近出现的3个发射峰分别对应于Nd3 的4F3/2→4I9/2、4F3/2→4I11/2和4F3/2→4I13/2跃迁;在Yb:GdAlO3晶体中,主要发射峰波长位于979、997和1028nm处,其中1028nm处为最强发射峰,对应于Yb3 的2F5/2→2F7/2跃迁.  相似文献   

7.
20 0 32 0 0 1 气体阀门美国专利 № 6 16 8136  (英 )2 0 0 32 0 0 2 气囊装置用气体发生装置和气囊充气方法美国专利 № 6 16 82 0 1  (英 )2 0 0 32 0 0 3 从氧和氮中分离氙的膜和使用该膜的方法美国专利 № 6 16 86 4 9  (英 )2 0 0 32 0 0 4 高温气体净化装置美国专利 № 6 16 86 5 0  (英 )2 0 0 32 0 0 5 电化气体纯化器美国专利 № 6 16 870 5  (英 )2 0 0 32 0 0 6 高氮不锈钢美国专利 № 6 16 875 5  (英 )2 0 0 32 0 0 7 高压放电灯用氧分配器美国专利 № 6 16 936 1  (英 )2 0 0 32 0 0 8 空气的分离美国…  相似文献   

8.
利用溶胶-凝胶法低温合成了钒掺杂硅酸盐氧基磷灰石La9(SiO4)6-x(VO4)xO1.5+0.5x(x=0、0.5和1),经XRD表征所得产品为磷灰石相.以电化学阻抗谱研究了其导电性能,V的掺杂使电导率大大提高,700℃时La9(SiO4)5(VO4)O2电导率为5.23×10-3S·cm-1,与未掺杂V的La9(SiO4)6O1.5相比提高了5倍.氧分压Po2=105~1Pa时,La9(SiO4)6O1.5的电导率不改变,而La9(SiO4)5(VO4)O2的电导率则随着氧分压的降低而稍有增加,表明钒的掺杂在体系中引入n型电子导电.  相似文献   

9.
应用微卫星标记分析柱花草的遗传多样性   总被引:16,自引:1,他引:16  
采用18个微卫星(SSR)DNA标记对42个柱花草品系进行了遗传多样性分析,共扩增出84条带,其中多态性带71条,平均多态性水平为84.5%。运用POPGEN32软件计算了各品系的等位基因频率、多态性信息含量、遗传杂合度、等位基因数及有效等位基因数,结果表明,抗病柱花草品系的遗传变异高于感病柱花草品系的遗传变异程度。用NTSYS-pc软件计算品系间的遗传相似系数,其变化范围为0.26~0.94。按非加权成对平均数法(UPGMA)进行聚类分析,获得了聚类树系图,以所有品系的平均遗传相似系数0.60为阈值,42个品系分为6类。这些结果表明,SSR技术是分析柱花草遗传多样性的有效方法。  相似文献   

10.
几种江蓠属海藻的ISSR标记分析   总被引:19,自引:0,他引:19  
用ISSR标记对几种江蓠属海藻进行了遗传多样性的研究。所用的7种实验材料包括4株龙须菜(其中3株分别来自中国青岛、委内瑞拉和南非,及1株青岛产龙须菜的高温选育种)和细基江蓠繁枝变型、真江蓠、芋根江蓠。用9条ISSR引物进行筛选,有6条可扩增出清晰可辨条带。这6条引物在7种材料中共扩增出151条带,其中117条(77.48%)表现出多态性。根据Nei等的遗传相似性系数(S)对7种材料进行分析,结果显示青岛产龙须菜与其选育种的相似性最高(S为0.939)。在3株不同产地龙须菜中,青岛龙须菜与南非龙须菜的遗传距离最近(S为0.643),委内瑞拉龙须菜与前两者的S分别为0.167和0.192,因此初步推断委内瑞拉龙须菜不属于龙须菜种。包括龙须菜(产自青岛或南非)在内的4种江蓠间的相似性系数在0~0.143之间。  相似文献   

11.
Permeable crystalline materialcan permeate into pores and cracks of concrete and catalyze the reaction between Ca(OH) 2and unhydrated cement to generate a great quantity needle non-soluble crystals, which can stop up the pores and cracks of concrete, and increase the impermeability of concrete. This paper reported the results of a study conducted to evaluate steel reinforcement corrosion of concrete specimens uncoated and coated with permeable crystalline material as well as mixed with the permeable crystalline material. The properties evaluated for corrosion test were water impermeability, water absorption, compressive strength and potential. The results of water impermeability, water absorption, compressive strength clearly showed that the permeable crystalline material could prohibit water, any soluble salts and moisture from penetrating the concrete to cause corrosion, leaking, and other problems, and it did increase the compressive strength, which was favorable for protection of corrosion of reinforcing steel. Moreover, it was concluded from the potential-time curve that the steel reinforcement of uncoated specimen was in the state of activation whereas that of other specimens coated and mixed with the permeable crystalline material was in the state of inactivation. Above all, it was indicated that the permeable crystalline materialis very effective to protect the steel reinforcement of concrete from corrosion.  相似文献   

12.
In this paper, the principle of spurting take off lacquer were introduced; and with orthogonal method the unsaturate polyester was prepared ;it was easy to peel airplane paint layer off with this material and equipment.  相似文献   

13.
Quality Study of Honeycomb Core Shaping Without Wax Stabilization   总被引:1,自引:0,他引:1  
Sandwich panels utilizing honeycomb cores result in lightweight, high-strength units that are very rigid. Indeed, they give honeycomb panels the best strength-to-weight ratio of any construction type available. Conventionally, the wax is poured into the cell of the core to strengthen the structure and prevent the core from being crushed during milling. Much time and labor is required to wax, mill, and clean the cores. In this study, the process of pouring wax into the core was skipped. In other words, the cutter directly milled the core. Experiments showed that the compressive strength of the core produced without wax stabilization was only 3% lower than that of cores produced using the traditional method. In addition, the drum peel strengths of cores produced without wax stabilization were 38.4% higher than cores produced using the traditional method, in which residual wax seriously polluted the adhesive. It was also found that eliminating the wax process can reduce costs.  相似文献   

14.
Polylactic acid (PLA) is one of the most widely used open source fused filament fabrication materials due to its ease of extrusion, biodegradability, and mechanical strength. The mechanical strength of PLA largely depends on the proper growth of its semi-crystalline structure, which can be severely impaired by a low rate of crystallization, particularly in open source printers. This can be further aggravated by the non-uniform thermal distribution of heat that causes improper curing among the extruded beads of the printing material. As a result, PLA printed on open source printers does not achieve the best mechanical properties. This research, for the first time, proposes an additive-free solution implemented through a detailed set of experimentation to improve the curing rate through in-process temperature variations to cure the joints among the beads. Scanning electron microscopy (SEM), differential scanning calorimetry (DSC) and Fourier transform infrared (FTIR) spectroscopy is used to confirm the improvements in the bead joints. This work is conducted in two phases of experiments. In the first phase, a full factorial ANOVA is used to investigate various process parameters and the important variables are used in the second phase to print test specimens in four different sets.  相似文献   

15.
In continuous hot-dip galvanization process the corrosion and chemical stability of the sink roll in the galvanizing bath are important problem which effects on the quality and productivity. In order to protect the sink roll the carbide cermet and/or ceramic coatings were deposited on the surface of the sink roll. The WC-, Cr3 C2-cermet coatings were deposited by high velocity oxygen fuel (HVOF) spray, respectively. The coating samples were immersed in molten Zn-alloy containing 50 wt % aluminum at 833 K for 24 hr and 144 hr, respectively. The inter-diffusion and inter-reaction of Zn, Al and elements in coating and corrosion behaviors of these coatings were investigated by XRD, SEM and EPMA etc. The corrosion mechanisms of the carbide cermet coatings and ceramic coatings in molten High Al-Zn-alloy were approached.  相似文献   

16.
In many situations, ice often adheres to a cooling solid surface, frequently causing serious accidents. It is critical to clarify the mechanism of ice adhesion to the cooling surface in order to prevent ice adhesion. In a past study, the shearing stresses of two kinds of test plates with a copper surface having the higher thermal conductivity were measured. The shearing stress corresponds to ice adhesion force. Both shearing stresses were significantly different; however, the cause remains unclear.Therefore, the present study focuses on an oxide layer as the main factor causing the difference of both shearing stresses; the influence of the oxide layer formed on shearing stress was discussed. And in the removal and reformation processes of the oxide layer, the time variation of the shearing stress was clarified. Moreover, the relationship between the state of the copper surface and the shearing stress was also clarified by surface analysis.  相似文献   

17.
During his inspection in Henan Province in May, Chinese President Xi Jinping stressed to "push forward the transformation from China Speed to China Quality, from Made in China to Created in China, and from Chinese Products to Chinese Brand". The "Three Transformations" are the specific requirements and goal of China's national development in the context that market should play a decisive role in the allocation of resources.  相似文献   

18.
正When you are enjoying the green,energy-saving and healthy lighting of IKEA LED luminaire,you may not realize that most of IKEA’s LED bulbs are produced by the LED luminaire smart manufacturing system of Hangzhou Zhongwei Photoelectricity CO.,LTD(ZVISION).No longer enclosed in the laboratory,the product standards are brought into the factory workshop and even to the entire production process.Thus,the standards are promoted and applied in a favorable way by enterprises,benefitting consumers with high quality products.  相似文献   

19.
正The international standard,ISO 8124-6,Safety of toys–Part 6:Certain phthalate esters in toys and children’s products,was officially issued by ISO worldwide on August 15.It was the first time for Chinese experts to lead the ISO standard development in toys industry.The standard was drafted by a group of Chinese experts from SAC/TC for toys and relevant Chinese departments and finally completed by a dedicated international group of experts.ISO 8124-6 makes a substantive breakthrough in the aspects of the scope of toys,limitation of plasticizer types,control of test cost,accuracy of test,operation convenience,etc.,offering the operational guidance for toy manufactures and testing laboratories.  相似文献   

20.
正SAC Administrator Tian Shihong led the Chinese delegation to visit the Spanish Association for Standardization and Certification(AENOR)on September 15,2014.Mrs.Begona Cristeto,the Vice-Minister of Industry,Energy and Tourism of Spain,and Mr.Avelino Brito,the President and CEO of AENOR greeted the delegation.The two sides had a deep and friendly discussion on strengthening the cooperation between China and Spain in the standardization field and promoting the standardization work to support the development of economy and trade in the two countries.  相似文献   

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