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采用连续延伸PCR方法克隆到粗糙脉孢菌(Neurospora crassa)漆酶基因,并将其克隆到表达载体pPIC9k,重组质粒经线性化、电激转化Pichia pastoris KM71,部分阳性克隆的PCR结果表明:漆酶基因已整合到巴斯德毕赤酵母染色体上,重组菌经甲醇诱导后3~5d产漆酶量最高,为2-3U/mL。 相似文献
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研究了碳源、氮源及诱导剂添加方式对白腐菌P.conchatus液体培养产漆酶的影响.结果表明,当碳源为葡萄糖,氮源为酵母膏,C/N比为15∶1时,P.conchatus合成漆酶酶活达到8795U·L-1;在初始培养基中,添加1.5%麦麸与1 mmol/L Cu2+共同诱导漆酶合成,酶活可达到51004U·L-1;将Cu2的添加时间改在发酵后第4d,添加量为3mmol/L,其他条件不变,漆酶酶活达到101968U·L-1,比在初始培养基中添加1 mmol/L Cu2+所达到的酶活高了近两倍. 相似文献
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悬浮培养霍山石斛原球茎合成活性多糖的研究 总被引:8,自引:0,他引:8
本文考察了基本培养基、碳源、氮源以及有机添加物对霍山石斛原球茎液体培养合成活性多糖的影响。在MS、B5、N6、SH及各自1/2基本培养基中,以1/2MS和N6最适多糖合成,继代培养30d,总多糖产率分别达到479.47mg/L和417.05mg/L。使用单一碳源时,葡萄糖对多糖的积累优于蔗糖和果糖,在30g/L时,总多糖产率达到1034.96mg/L。在一定浓度范围内,氮源种类对多糖的合成影响不太明显,但氮源浓度是主要冈素,以10mmol/L最佳。另外,100g/L的马铃薯提取液与100g/L的香蕉提取液均有利于多糖的合成。 相似文献
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微生物发酵法生产番茄红素是直接提取和化学合成的有效替代策略。以油酸为外源脂肪酸,探究其对粗糙脉孢菌发酵产番茄红素的影响,通过响应面法优化确定最佳培养条件,并进一步探究油酸对番茄红素合成相关酶的影响。结果表明:在含油酸的培养基中,粗糙脉孢菌的番茄红素产量显著增加;在油酸质量浓度0.90 g/L、振荡速率100 r/min、振荡培养温度34℃、静置培养温度30℃条件下,番茄红素产量达到4.21 mg/L,是空白对照组的2.77倍。培养基中油酸的添加不仅促进了粗糙脉孢菌生物量的积累,菌体内乙酰辅酶A的水平也显著增加;同时,与番茄红素合成相关酶(HMG-CoA合成酶、HMG-CoA还原酶、香叶基香叶基焦磷酸合酶)的水平亦显著升高。结果表明油酸的添加可供利用更多的乙酰辅酶A进入甲羟戊酸途径,并进一步在上述酶的诱导作用下促进番茄红素的合成。 相似文献
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从甘蔗渣滤泥中筛得一株具有漆酶活性的细菌SYBC star X,通过16S r RNA基因序列分析,并结合生理生化实验及形态学观察,鉴定为考克氏菌属(Kocuria);利用液态发酵法研究了碳源、氮源、金属离子对其发酵产漆酶的影响,确定菌株SYBC star X的最优碳源为15 g/Lα-乳糖,最佳氮源为40 g/L大豆蛋白胨,最适铜离子和锰离子的浓度分别为3 mmol/L和0.4 mmol/L。 相似文献
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Textural Properties of Cold-set Gels Induced from Heat-denatured Whey Protein Isolates 总被引:2,自引:0,他引:2
A 9% whey protein (WP) isolate solution at pH 7.0 was heat-denatured at 80°C for 30 min. Size-exclusion HPLC showed that native WP formed soluble aggregates after heat-treatment. Additions of CaCl2 (10–40 mM), NaCl (50–400 mM) or glucono-delta-lactone (GDL, 0.4–2.0%, w/v) or hydrolysis by a protease from Bacillus licheniformis caused gelation of the denatured solution at 45°C. Textural parameters, hardness, adhesiveness, and cohesiveness of the gels so formed changed markedly with concentration of added salts or pH by added GDL. Maximum gel hardness occurred at 200 mM NaCl or pH 4.7. Increasing CaCl2 concentration continuously increased gel hardness. Generally, GDL-induced gels were harder than salt-induced gels, and much harder than the protease-induced gel. 相似文献
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Anne Theobald Catherine Simoneau Philippe Hannaert Patrizia Roncari Anna Roncari Thomas Rudolph Elke Anklam 《Food Additives & Contaminants》2000,17(10):881-887
The levels of bisphenol-F-diglycidyl ether (BFDGE) were quantified as part of a European survey on the migration of residues of epoxy resins into oil from canned fish. The contents of BFDGE in cans, lids and fish collected from all 15 Member States of the European Union and Switzerland were analysed in 382 samples. Cans and lids were separately extracted with acetonitrile. The extraction from fish was carried out with hexane followed by re-extraction with acetonitrile. The analysis was performed by reverse phase HPL C with fluorescence detection. BFDGE could be detected in 12% of the fish, 24% of the cans and 18% of the lids. Only 3% of the fish contained BFDGE in concentrations considerably above 1mg/kg. In addition to the presented data, a comparison was made with the levels of BADGE (bisphenol-A-diglycidyl ether)analysed in the same products in the context of a previous study. 相似文献
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Microgels are ‘soft’ microscopic cross-linked polymeric particles that are being increasingly exploited in a variety of industries for rheology control, encapsulation and targeted delivery. They are valued because of the ability to tune their functionality to address specific applications in oil recovery, coatings, drug delivery, cosmetics, personal care and foods. Food microgels are typically biopolymer hydrogels in the form of microspheres, nanospheres (also called nanogels), spheroids and fibres. The utilisation of engineered microgels in foods has so far been limited, despite their great potential to address several needs in the food industry, including: satiety control, encapsulation of phytonutrients and prebiotics, texture control for healthier food formulations (e.g. reduced fat products), and targeting delivery to specific areas in the digestive tract. We review the scientific and patent literature on the utilisation and manufacturing methods for producing microgels with an emphasis on micro-hydrogels for food applications. 相似文献
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Joubert and Burns prepared a large number of fractions from the high-sulphur proteins of wool and estimated their molecular weights and amino-acid compositions. Their data have been re-examined in order to look for statistically significant interrelations between amino acids and between the proportion of various amino acids and molecular weight. Statistical analysis of the data is also used to examine the credibility of some hypotheses concerning the mechanism of keratin biosynthesis and to provide further evidence for the existence of families of proteins within the high-sulphur fractions of wool. 相似文献
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A. Boenke 《Food Additives & Contaminants》2001,18(12):1135-1140
The European Commission's, Quality of Life Research Programme, Key Action 1—Health, Food & Nutrition is mission-oriented and aims, amongst other things, at providing a healthy, safe and high-quality food supply leading to reinforced consumer confidence in the safety of European food. Its objectives also include the enhancing of the competitiveness of the European food supply. Key Action 1 is currently supporting a number of different types of European collaborative projects in the area of risk analysis. The objectives of these projects range from the development and validation of prevention strategies including the reduction of consumers risks; development and validation of new modelling approaches; harmonization of risk assessment principles, methodologies, and terminology; standardization of methods and systems used for the safety evaluation of transgenic food; providing of tools for the evaluation of human viral contamination of shellfish and quality control; new methodologies for assessing the potential of unintended effects of genetically modified (genetically modified) foods; development of a risk assessment model for Cryptosporidium parvum related to the food and water industries; to the development of a communication platform for genetically modified organism, producers, retailers, regulatory authorities and consumer groups to improve safety assessment procedures, risk management strategies and risk communication; development and validation of new methods for safety testing of transgenic food; evaluation of the safety and efficacy of iron supplementation in pregnant women; evaluation of the potential cancer-preventing activity of pro- and pre-biotic ('synbiotic') combinations in human volunteers. An overview of these projects is presented here. 相似文献
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《肉类研究》2014,(12)
<正>We are pleased to announce the launch of a new international peer-reviewed journal-Food Science and Human Wellness,ISSN 2213-4530,which is an open access journal,produced and hosted by Elsevier B.V.on behalf of Beijing Academy of Food Sciences.Food Science and Human Wellness is an international peer-reviewed English journal that provides a forum for the dissemination of 相似文献
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《印刷工业》2014,(9):94-95
In the 2014 China(Shanghai)International Printing Week,Director Wang Yanbin released the latest data about development of Chinese printing industry in 2013.According to statistics,in 2013,the total output value of Chinese printing industry exceeded 1trillion Yuan for the first time,reaching 1.03985 trillion Yuan.There were 105,000 printing enterprises in China,employees were 3.415 million.The total asset was 1.06247 trillion Yuan; 相似文献
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The State Council Issued "Directory of Government Approved Investment Projects(2013 Edition)" 下载免费PDF全文
《造纸信息》2014,(8)
正On December 2nd,2013,the State Council issued the notification of"Directory of Government Approved Investment Projects(2013 Edition)"(hereafter referred to as"notification").It is pointed out in the"notification"that in order to further deepen reforms in investment systems and administrative examination and approval systems,simplify administrative procedures and delegate powers to lower levels,earnestly 相似文献
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《纺织机械》2014,(3)
正Among the 1600 exhibitors who take apart in the ITMA ASIA+CITME2014 2/3 are Chinese manufactures.If the numerous figures failed to attract your attention,the increase of quality should draw your focus.To adopt the demand of developing textile machine market,domestic textile machinery enterprises now follow the slogan of"technology drives development"to enhance product competitiveness.Our domestic sellers will showcase product ranging from spinning,weaving,dyeing and printing, 相似文献
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《造纸信息》2014,(8):99-100
On December 24th, 2013, the meeting on the selection of top 10 news of China's paper industry 2013 sponsored by 〈China Paper Newsletters〉 was held in Beijing. The yearly selection of the top l0 news, which began in 2000, has become a brand activity widely recognized in the industry thanks to the support from the authorities at all levels and public participation. 相似文献