酶联免疫吸附法在食品微生物检测中的应用分析

在现代生活中,食品微生物检测是人们关注的重点之一。由于传统技术方法的限制,现有的食品微生物检测方法存在着一定的问题和不足。为了满足未来发展的要求,需要推动酶联免疫吸附法在食品微生物检测中的应用。本文通过分析食品微生物检测的技术要求,对酶联免疫吸附法的应用价值进行了探讨。以此为基础,本文结合时代技术发展的特点,对酶联免疫吸附法的应用特点和应用要点进行了研究。这些研究对酶联免疫吸附法的应用和食品微生物检测的发展有着重要意义,有很高的现实价值。     

酶联免疫吸附试验在食品检测中的应用

酶联免疫吸附试验在食品检测中能够达到非常好的效果。基于此,本文阐述了酶联免疫吸附试验技术存在的应用问题及解决办法,同时提出了酶联免疫吸附试验在各类食品检测中的具体应用,包括检测食品中的农药残留、检测食品是否为转基因食品、检测食品中的病原微生物含量等。通过论述以上内容,来为食品检测技术人员提供一些参考。     

百菌清酶联免疫吸附检测方法的影响因素

以方阵滴定法筛选适合百菌清酶联免疫吸附检测的包被抗原和抗体浓度,同时研究反应缓冲体系和pH值等因素对酶联免疫吸附方法的影响以及百菌清多克隆抗体的亲和性和特异性,建立蔬菜中百菌清残留检测的间接竞争酶联免疫吸附法。结果表明:方法最低检测限(IC20)为0.0123ng/mL,回收率为84.0%～89.8%,变异系数为3.0%～7.6%,与百菌清结构类似化合物交叉反应率均小于0.01%,可在试剂样品检测中应用。     

酶联免疫吸附试验在食品检测中的应用

本文针对酶联免疫吸附试验在食品检测中的有效应用进行分析,包括农药残留检测、违禁药物检测、生物毒素检测等;随后介绍了酶联免疫吸附试验在食品检测中的具体问题以及相应的解决措施,希望能给相关人士提供一些参考。     

化学合成类抗球虫药残留检测方法研究进展

文章综述了化学合成类抗球虫药残留的检测方法,重点介绍了高效液相色谱串联质谱法、酶联免疫吸附测定法、免疫层析试纸法、表面增强拉曼光谱法等的研究进展,总结了检测方法发展现状与趋势,并对其未来发展方向进行了展望.     

酶联免疫吸附法及其在食品分析中的应用

简单介绍了酶联免疫吸附法(ELISA),并且就其在食品分析中的应用进行了较详细的评述,主要包括ELISA用于食品微生物、食品中的毒素、残留农药和其他成分的检测。     

果蔬中农药残留的检测方法

随着我国社会经济的发展和人们生活水平的提升,果蔬农药残留问题成为了人们日常关心的重点问题。长期食用有农药残留的果蔬食物,不但会导致慢性中毒影响身体健康,还会影响下一代的生长发育。本文将针对酶抑制法、酶联免疫法、生物传感器法和红外光谱法等几种果蔬农药残留检测方法进行分析,并介绍不同检测方法的优缺点。     

免疫学技术在食品安全快速检测中的应用研究进展

随着人们对于食品安全问题的关注程度不断增加,食品安全快速检测方法得到了广泛应用。目前常用的食品安全快速检测技术包括免疫学技术、酶抑制技术、传感器技术、生物芯片技术等。免疫学检测技术具有灵敏度高、特异性强、方便、快速和经济等优点,在食品安全快速检测中发挥了重要的作用。免疫学技术在食品安全领域广泛应用的主要有免疫吸附法和免疫层析法两大体系,其中免疫吸附法以酶联免疫吸附检测法(enzyme-linked immunosorbent assay,ELISA)最常用,而免疫层析法则以胶体金免疫层析技术(colloidal gold immunochromatographic assay,GICA)为代表。本文介绍了免疫学技术在食品安全快速检测中应用的原理及特点,并对酶联免疫吸附检测技术和胶体金免疫层析技术近几年来在食品安全快速检测中的应用进展进行了综述。     

酶联免疫吸附测定技术在食品农残快检中的应用探讨

酶联免疫吸附测定技术是将抗原抗体反应的高度特异性和酶催化充分结合的高效检测手段。本文主要探讨酶联免疫吸测定技术(ELISA)在食品和食用农产品农药残留快速检测实验中的应用。     

动物源性食品安全快速检测及酶联免疫吸附方法的应用

本文介绍了动物源性食品安全的定义和存在的主要问题,重点阐述了酶联免疫吸附方法(ELISA)在兽药残留、农药残留、动物疫病、微生物、动物毒素快速检测中的应用.     

酶联免疫吸附法快速测定不同样品 基质中三聚氰胺

目的 探求不同样品基质中三聚氰胺残留量的快速检测方法.为快速筛查不同种类食品中非法添加三聚氰胺提供技术保障.方法 采用酶联免疫吸附法在奶制品(奶粉、液态奶)、成品饲料(鸡饲料、猪饲料)、饲料原料(鱼粉、肉骨粉、豆粕、麸皮)、肉类(鸡肉、猪肉、内脏)等样品基质中添加一定浓度的三聚氰胺进行测定,并对检测结果进行分析.结果 酶联免疫试剂盒对奶制品和肉类检出限均能达到1.0 mg/kg;对成品饲料基质中的三聚氰胺的检测,检出限可达到2 mg/kg,而对饲料原料中的三聚氰胺的检测,检出限都不能达到2 mg/kg.结论 对奶制品中的三聚氰胺检测完全符合我国的临时限量标准;对成品饲料中的三聚氰胺残留的检测同样符合其限量标准(2.5 mg/kg),而对饲料原料中的三聚氰胺的检测,由于不同基质中检出限不同,不能直接采用酶联免疫法进行快速筛查;酶联免疫法也适用于肉类中的三聚氰胺的检测.     

酶联免疫吸附法和DNA检测法在肉类鉴别中的应用

肉类食品是我国人民食品消费的主要组成部分,企业和经营单位为了追逐利润,时有将价格便宜的肉品掺入或替代高价格的肉品中进行加工和销售。为保护消费者对食品消费的知情权和规范肉类市场的秩序,食品生产、流通等监管部门应对肉类识别技术进行系统的分析和研究,进而建立相应的规范识别方法。目前用于肉类掺假鉴别的方法有组织学、化学、免疫学和基于DNA序列的检测方法等。本文主要针对ELISA检测方法和DNA检测方法在肉类识别中的应用进行阐述分析,并对我国肉类识别标准方法的缺陷进行分析。     

Detection of Konjac glucomannan by immunoassay

Konjac glucomannan is a hydrocolloid that has been used in food applications. The European ban on the use of Konjac glucomannan means that the detection and analysis has potential applications in the food industry, particularly detection of food adulteration. The aim of this work was to develop an assay capable of detecting Konjac glucomannan as an isolated sample and within food matrices. An indirect competitive ELISA was developed utilising a polyclonal antibody raised against Konjac glucomannan. The ELISA was found to be specific for Konjac glucomannan and sensitive, with a detection limit of 0.1 ng mL^?1. Increasing salt concentration and freeze/thaw cycles did not affect the performance of the assay. The ELISA was able to detect Konjac glucomannan in admixtures with other gums and also in confectionery that had been spiked with Konjac glucomannan. The ELISA has potential as a kit for the differentiation of Konjac glucomannan from other hydrocolloids and detection in food.     

一起食物中毒事件中产独特肠毒素表型的金黄色葡萄球菌病原学分析

目的对一起疑似为金黄色葡萄球菌所导致的食物中毒事件进行葡萄球菌肠毒素检测,结合金黄色葡萄球菌病原学分析,为明确食物中毒诊断提供依据。方法根据流行病学调查,采用ELISA方法对可疑食物进行葡萄球菌肠毒素检测,同时对可疑食物和患者呕吐物进行金黄色葡萄球菌分离,运用Vitek2 Compact全自动细菌鉴定仪和血浆凝固酶试验鉴定为金黄色葡萄球菌,采用脉冲场凝胶电泳(PFGE)对病原菌进行同源性分析,以ELISA方法对检出的金黄色葡萄球菌菌株进行肠毒素检测,用PCR方法对肠毒素基因进行分型。结果食物和患者样品中分别分离出2和11株金黄色葡萄球菌,PCR方法及ELISA方法对肠毒素分型结果显示,其中12株同时存在SEA、SEB、SED、SEE 4种肠毒素及相关基因,PFGE聚类分析显示,其中12株产肠毒素金黄色葡萄球菌具有高度同源性。结论本起食物中毒事件为具有独特肠毒素表型的金黄色葡萄球菌导致,在金黄色葡萄球菌中毒实验室调查过程中,肠毒素检测结合病原菌溯源分析可以为相关公共卫生事件提供科学依据。     

大豆过敏原夹心ELISA和竞争ELISA方法的建立及其在加工食品中应用研究

本文以大豆混合过敏原为目标,建立了快速、便捷检测大豆过敏原的夹心酶联免疫吸附方法（sandwich-enzyme linked immunosorbent assay）和间接竞争酶联免疫吸附方法（indirect competitive enzyme-linked immunosorbent assay）,通过实际加工样品的回收实验、加标食品回收实验以及对真实食物样本的检测,对这两种方法进行了比较,确定了各自的适用范围。结果表明,夹心ELISA方法标准品浓度在0.0078~30 μg/mL范围内呈现出良好的线性关系,曲线方程为y=0.2333x+0.0692,决定系数R2=0.995。竞争ELISA方法的检测范围为10~100000 ng/mL,最低检测限为10 ng/mL。对购入橙汁进行加标回收实验,夹心ELISA检测后的回收率要高于竞争ELISA检测后的回收率,达100%以上;而对成分和加工方式都比较复杂的巧克力、牛肉酱、面包或蛋糕来说,竞争ELISA检测后的回收率要高于夹心ELISA检测后的回收率。对发酵类食物进行检测,竞争ELISA方法检测到的浓度要高于夹心ELISA,而对成分比较简单的食物比如芝麻糊、豆奶等进行检测时,夹心ELISA的检测浓度要略高于竞争ELISA。综上,竞争ELISA方法更适用于食物基质复杂,经过深度加工的食品,而夹心ELISA方法更适用于食物成分简单,轻加工后的食品,两种方法在各自的适用范围内均能实现较准确的检测。     

Effect of protein glycation in the presence or absence of wheat proteins on detection of soybean proteins by commercial ELISA

Soybean (Glycine max) is the world's primary provider of protein and oil and is widely used in foodstuffs. However, the use of soybean in foodstuffs might pose a serious threat to allergic consumers since some proteins can cause allergic reactions. To date mostly ELISA methods are used for testing contamination of foodstuffs with soybean. In view of the complexity regarding allergen detection in foodstuffs and appropriate food product labelling, the aim of this study was to investigate the impact of the Maillard reaction on the detectability of soybean proteins using commercial ELISA kits. Accumulation of protein-bound carbonyls, modification of reactive lysine residues and severe aggregation as a result of incubation with glucose, in the presence or absence of soluble wheat proteins, were recorded. Moreover, detection of soybean proteins by means of three commercial ELISA kits was strongly altered and was highly dependent on the type of kit used.     

食源性致病菌生物快速检测技术研究进展

随着人民生活水平的提高和食品行业的发展,食品安全问题频繁发生,成为人们的重点关注问题。食源性致病菌是造成食品安全问题的主要因素之一,使用可靠、快速、有效的检测方法对保证食品安全至关重要。现如今生物技术发展迅速,其在食品致病菌检测中的应用是当前的研究热点。本文综述了免疫学、生物学、生物传感器技术等基于生物技术在食品中致病菌的技术开发和应用研究进展,并提出双功能抗体在食品检测领域的研究前景,以期为食品致病菌的微生物快速检测与筛查技术提供方法以参考。     

Influence of baking time and matrix effects on the detection of milk allergens in cookie model food system by ELISA

Milk allergens are common allergens occurring in foods, therefore raising concern in allergic consumers. Enzyme-linked immunosorbent assay (ELISA) is, to date, the method of choice for the detection of food allergens by the food industry although, the performance of ELISA might be compromised when severe food processing techniques are applied to allergen-containing foods. In this paper we investigated the influence of baking time on the detection of milk allergens by using commercial ELISA kits. Baked cookies were chosen as a model food system and experiments were set up to study the impact of spiking a matrix food either before, or after the baking process. Results revealed clear analytical differences between both spiking methods, which stress the importance of choosing appropriate spiking methodologies for method validation purposes. Finally, since the narrow dynamic range of quantification of ELISA implies that dilution of samples is required, the impact of sample dilution on the quantitative results was investigated. All parameters investigated were shown to impact milk allergen detection by means of ELISA.     

Development of a chemiluminescent enzyme‐linked immunosorbent assay for five sulfonamide residues in chicken muscle and pig muscle

BACKGROUND: An enzyme‐linked immunosorbent assay (ELISA) based on polyclonal antibodies with enhanced chemiluminescent (ECL) detection of sulfonamides in food samples has been optimised and characterised. The specificity of the assay was assessed by determining cross‐reactivities with a set of 16 sulfonamides. The aim of this study was to develop a method for determining sulfonamides with high sensitivity. RESULTS: The sensitivity of the developed ECL‐ELISA was higher than that of colorimetric ELISA. The sensitivities of five of the sulfonamides (sulfisozole, sulfathiazole, sulfameter, sulfamethoxypyridazine and sulfapyridine) ranged from 0.73 to 2.92 µg L^?1, with limits of detection of 0.10–0.43 µg L^?1. The coefficients of variation of intra‐assay and inter‐assay studies carried out over 5 days were mostly less than 10%. Recovery studies of chicken muscle and pig muscle were performed with simple and rapid extraction. Good recoveries (62.1–110.3%) were achieved and the results correlated well with those obtained using high‐performance liquid chromatography analysis. CONCLUSION: This study has provided an effective analytical technique for the rapid and reliable determination of residues of sulfisozole, sulfathiazole, sulfameter, sulfamethoxypyridazine and sulfapyridine in food samples with high sensitivity. To the authors' knowledge, this is the first report on chemiluminescent ELISA for sulfonamide analysis. Copyright © 2008 Society of Chemical Industry     

Detection and quantification of goat's cheese in ewe's cheese using a monoclonal antibody and two ELISA formats

A stable hybridoma cell line (B2B) has been produced secreting a monoclonal antibody (MAb) specific for the s α_s2‐casein of goats. The MAb B2B was used in two enzyme‐linked immunosorbent assays (ELISA) formats for the detection and quantification of the presence of goat's cheese in ewe's cheese samples. In the indirect ELISA format the limit of detection was 1–25% (w/w) substitution of ewe's cheese samples by goat's cheese. Afterwards, a competitive indirect ELISA was successfully developed for the detection of 0.5 to 25% (w/w) of goat's cheese in ewe's cheese samples. This competitive indirect ELISA is a very sensitive assay, can be performed in less than 5 h and is not influenced by the ripening process in cheese. © 1999 Society of Chemical Industry