共查询到19条相似文献,搜索用时 115 毫秒
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本文主要对植物甾醇与甾醇酯的薄层色谱法、气相色谱法、高效液相色谱法的分离分析方法进行研究探讨,为甾醇与甾醇酯的分离分析提供切实可行的参考。 相似文献
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几种常见坚果植物甾醇组成及含量测定 总被引:1,自引:0,他引:1
采用毛细管气相色谱法,对葵花籽、花生、核桃及松子中植物甾醇组成进行分析并测定其含量。结果表明:四种坚果中均含有菜油甾醇和β-谷甾醇,均未检测到菜籽甾醇及豆甾醇;其甾醇总含量以松子最高,葵花子和花生次之,最低为核桃。同时,在所有样品中测出三种甾醇中均以β-谷甾醇含量最高。 相似文献
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气相色谱法测定小麦胚芽油、大豆油和大蒜油中植物甾醇的组成和含量 总被引:1,自引:1,他引:0
目的建立气相色谱法检测小麦胚芽油、大豆油和大蒜油中植物甾醇的组成和含量。方法采用乙醇氢氧化钾-甲基叔丁基醚溶液水浴上皂化,异辛烷萃取,毛细管气相色谱法分离植物甾醇的分析方法。结果植物甾醇各组分在相应的浓度范围内呈现良好的线性关系,方法相关系数为0.9997~0.9999,平均回收率在96.8%~101.3%,相对标准偏差在0.4~1.0%之间。结论小麦胚芽油、大豆油和大蒜油中植物甾醇主要由菜油甾醇、豆甾醇、β-谷甾醇组成,其中β-谷甾醇含量最高,且小麦胚芽油中植物甾醇的总量最高。 相似文献
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徐明明郑璐侠吕晶邵泓陈钢 《中国油脂》2016,(1):84-87
探讨了甾醇组成分析结合化学计量学识别植物油脂种类的可行性。从植物油脂中提取不皂化物,并经液相色谱分离,再经衍生化后通过气相色谱测定了8种植物油脂共21批样品的甾醇组成,并以15种甾醇组分的含量作为基础数据,进行聚类分析与主成分分析。结果表明,2种化学计量学方式对甾醇组成的分析结果基本一致,都可以对植物油脂进行识别分析。因此,化学计量学是植物油脂中的甾醇组成分析的有效方法,甾醇组成可作为植物油脂种类识别的测量指标之一。 相似文献
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烟草中游离植物甾醇的GC/MS/SIM分析 总被引:1,自引:1,他引:1
建立了一种采用二氯甲烷提取、微孔滤膜过滤、气相色谱/质谱/选择离子监测(GC/MS/SIM)定量测定烟草中游离植物甾醇的方法,并采用该法测定了部分烟叶样品、霉变烟叶和部分卷烟样品中的游离植物甾醇含量。结果表明:①该法的检测限为5.12~8.43ng/g,回收率在93%~103%之间,RSD<6%;②香料烟的麦角甾醇含量最高,其次是烤烟,白肋烟最低;③卷烟烟丝中的游离植物甾醇含量与其烟气中苯并[a]芘的释放量呈显著正相关。结论:该法适合批量烟叶样品中甾醇的定量分析。 相似文献
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建立气相色谱法同时测定食品中植物甾醇和胆固醇含量的分析方法,测定植物甾醇含量较高的常见植物油和同时含有胆固醇及植物甾醇的样品。采用5α-胆甾烷为内标参照物,样品经皂化后提取,浓缩后正庚烷定容上机进样分析。胆固醇和植物甾醇在0.001~2.5 mg/mL质量浓度范围内线性良好,线性相关系数R2均大于0.99。方法的检出限为0.3 mg/100 g,定量限为1.0 mg/100 g,该方法相较于液相色谱法能有效将胆固醇和植物甾醇进行分离,具有步骤简单、重复性好、准确度和灵敏度高等特点。 相似文献
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为了对元宝枫籽油的精炼提供指导,分析了精炼过程中元宝枫籽油的基本理化指标、脂肪酸和有益油脂伴随物(植物甾醇、生育酚)的变化情况。结果表明:随着精炼工序的进行,元宝枫籽油的酸值、过氧化值和氧化诱导期显著降低(p<0.05),脂肪酸组成变化不显著(p>0.05),植物甾醇和生育酚两种有益油脂伴随物的含量显著降低(p<0.05),分别由毛油的950.3 mg/kg和1 107.4 mg/kg降至脱臭油的569.9 mg/kg和743.4 mg/kg,损失率分别为40.0%、32.9%。研究结果说明精炼能够显著影响元宝枫籽油的品质,应有效控制脱臭条件,尽量减少有益油脂伴随物的损失。 相似文献
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Detection of adulteration of pumpkin seed oil by analysis of content and composition of specific Δ7-phytosterols 总被引:1,自引:0,他引:1
A simple and accurate method for the determination of phytosterols by capillary gas chromatography was developed for the
analysis of the seeds and oil of the pumpkin Cucurbita pepo L., the naked seed variety growing in the southern Styrian parts of Austria. After extraction of the oil and saponification,
the remaining unsaponifiable material was isolated and purified using silica gel columns. For greater volatility, the sterols
were derivatised to trimethylsilylethers, and gas chromatography was performed on a column of high polarity, allowing separation
of the Δ5-sterols from the Δ7-sterols and thus yielding a typical chromatographical pattern. Quantification of the phytosterols
using 5α-cholestan or cholesterol as internal standards led to a method of high accuracy. Comparison of the chromatographic
pattern of the phytosterol fraction of pumpkin seed oil with those of other vegetable oil samples permitted the use of this
method to detect adulteration of pumpkin seed oil.
Received: 20 April 1998 相似文献
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以鹰嘴豆为原料,在单因素实验的基础上,利用响应面分析法对超声波辅助提取植物甾醇的工艺条件进行优化,使用柱层析法对提取物进行分离纯化,使用气相色谱质谱联用法(GC-MC)对主要甾醇的种类进行鉴定,并对鹰嘴豆中植物甾醇的抑菌效果进行研究。结果表明,鹰嘴豆植物甾醇的最佳提取工艺为:超声功率450 W,提取时间7 min,液料比10:1 mL/g,此时提取率为(57.99±3.37) mg/100 g·dw。柱层析法分离纯化植物甾醇的回收率为53.44%。经GC-MS鉴定,分离纯化后鹰嘴豆中植物甾醇以β-谷甾醇(80.43%)、菜油甾醇(12.48%)与豆甾醇(7.09%)为主。抑菌实验结果显示,鹰嘴豆植物甾醇对于大肠杆菌、枯草芽孢杆菌、金黄色葡萄球菌以及沙门氏菌均有量效关系。本次研究为进一步开发利用鹰嘴豆中植物甾醇提供一定的参考。 相似文献
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HPLC-DAD结合交替三线性分解二阶校正法测定荔枝果肉中游离植物甾醇 总被引:1,自引:0,他引:1
为快速测定荔枝果肉中游离植物甾醇含量,建立高效液相色谱-二极管阵列检测器结合基于交替三线性分解算法的二阶校正方法。基本色谱条件:岛津ODS-SP柱(150 mm×4.6 mm,5 μm),流动相体积分数95%乙腈溶液,检测波长范围为190~340 nm,柱温30 ℃,流速1.0 mL/min,进样量20.0 μL。结果表明,‘兰竹’和‘乌叶’荔枝样品中菜油甾醇含量分别为24.1 μg/g和27.4 μg/g,两种荔枝样品豆甾醇含量分别为25.8 μg/g和26.7 μg/g,菜油甾醇和豆甾醇加标回收率分别为(95.25±0.78)%和(96.83±1.01)%。所建立的方法简单、准确、灵敏度高且可靠,可用于检测荔枝果肉中游离植物甾醇含量。 相似文献
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Simultaneous Analysis of Tocopherols, Cholesterol, and Phytosterols Using Gas Chromatography 总被引:1,自引:0,他引:1
ABSTRACT: A method for the simultaneous analysis of tocopherols and sterols was developed. Lipids were extracted with the Folch method, saponified by KOH-ethanol, and then tocopherols, cholesterol, and phytosterols were extracted with hexane. The extracted samples were dried under a nitrogen stream, derivatized using trimethylsilyl compounds, and then subjected to a gas chromatography. The recovery rates for cholesterol, stigmasterol, and sitosterol were about 100%, but recovery rates for tocopherols were low (25% forδtocopherol and 66% for γ-tocopherol) and varied according to compound structures. However, the recovery rates for δ- and γ-tocopherols increased to about 100% when the amounts of water and hexane were increased to 15 mL at the extraction step after saponification. 相似文献
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Supradip Saha S. Walia Aditi Kundu Charanjit Kaur Jashbir Singh R. Sisodia 《International Journal of Food Properties》2013,16(7):1535-1545
A gas chromatography-mass spectrometry method was developed for the quantitative determination of capsaicinoids, vitamin E, and phytosterols in chili peppers using a simple extraction technique for rapid screening. These components were extracted with acetonitrile and were injected into gas chromatography attached with mass spectrometry. The mean recovery values for triplicate analysis were between 90.6–99.7%. Besides major capsaicinoids (capsaicin, dihydrocapsaicin, nordihydrocapsaicin), four more minor capsaicinoids (nonivamide, nornordihydrocapsaicin, homodihydrocapsaicin I and II) were detected in chili samples. α-Tocopherol was also detected in the same run along with three phytosterols (campesterol, γ-sitosterol, and stigmasterol). Variations were observed across the chili sample in all of the constituents except a few minor capsaicinoids. The method was suitable for simultaneous estimation of capsaicinoids, vitamin E, and phytosterols in a single run from any types of pepper. Thus, the method is effective for rapid screening of peppers for its nutraceutical composition using single solvent extraction. 相似文献